1999
DOI: 10.1128/mcb.19.7.5166
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Sister Chromatid Exchanges Are Mediated by Homologous Recombination in Vertebrate Cells

Abstract: Sister chromatid exchange (SCE) frequency is a commonly used index of chromosomal stability in response to environmental or genetic mutagens. However, the mechanism generating cytologically detectable SCEs and, therefore, their prognostic value for chromosomal stability in mitotic cells remain unclear. We examined the role of the highly conserved homologous recombination (HR) pathway in SCE by measuring SCE levels in HR-defective vertebrate cells. Spontaneous and mitomycin C-induced SCE levels were significant… Show more

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Cited by 394 publications
(324 citation statements)
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“…24 SCEs can be monitored by the incorporation of bromodeoxyuridine (BrdU) for two DNA duplication cycles, which leads to a staining gap in one chromatid and a gain of staining in the corresponding sister chromatid (Fig. 3C).…”
Section: Discussionmentioning
confidence: 99%
“…24 SCEs can be monitored by the incorporation of bromodeoxyuridine (BrdU) for two DNA duplication cycles, which leads to a staining gap in one chromatid and a gain of staining in the corresponding sister chromatid (Fig. 3C).…”
Section: Discussionmentioning
confidence: 99%
“…The major cellular consequence of a BLM defect is an increase in homologous recombination (SCE and homologous chromatid interchanges) and in the rate of widespread mutations (German, 1993). It has been recently con®rmed that homologous recombination is the mechanism responsible for SCE, indicating that DNA lesions accompanying replication can be repaired by homologous recombination using the nascent sister chromatid as template (Sonoda et al, 1999). Thus, when an unrepaired lesion blocks replication fork progression, two alternative mechanisms can take place: the lesion can be by-passed by translesion synthesis or repaired by homologous recombination through sister chromatid exchanges.…”
Section: Discussionmentioning
confidence: 99%
“…Sister chromatid exchanges are induced by many factors that disturb DNA structure, metabolism and repair mechanisms. The frequency of SCE increases as a result of DNA damage by factors that inhibit the progression of the replication fork in replicons (Sonoda et al 1999). Faulty systems of DNA damage repair (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…Faulty systems of DNA damage repair (e.g. homologic recombination) also cause SCEs (Sonoda et al 1999, Wilson & Thompson 2007.…”
Section: Introductionmentioning
confidence: 99%