Sistema em fluxo para determinação espectrofotométrica de uréia em plasma de sangue animal empregando leguminosa como fonte natural da enzima urease

Luca, Gilmara Caseri de; Reis, Boaventura F.

doi:10.1590/s0100-40422001000200008

Cited by 8 publications

(5 citation statements)

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“…Enzymes have been remarkably accepted as biocatalysts in diverse sectors owing to green chemistry and their substrate specificity. [1][2][3][4][5][6][7][8] The enzymes are used industrially, among others, in food products, cellulose and paper, animal feed, textile cosmetics, detergents and the pharmaceutical industry. [9][10][11][12][13][14][15][16] To increase the use of enzymes as industrial biocatalysts, it is necessary to obtain stable preparations of the enzymes with an improved operational stability, 17 their application renders into lower demand for raw materials, chemicals and energy, heir specificity and high selectivity, and they drive into cleaner industrial processes.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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The enzymatic processes are increasingly highlights, especially in the synthesis of chemical products with high added value. The enzyme immobilization can improve industrial biocatalytic processes. The immobilization of enzymes provides the production of efficient, stable biocatalysts, possibility of reuse and easy purification of the products, when compared to the free enzymes. There is a growing research for more efficient methods of enzyme immobilization. In this context, the choice of support and immobilization strategy can significantly improve the final enzymatic properties. In this review paper, we aimed to discuss the versatility of biocatalysts immobilized enzymes design, focusing on the opportunities and disadvantages for each method presented. They discussed the recent development of enzyme immobilization methods and applications relating the final properties of the produced biocatalysts with the desired goals.

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Section: Enzyme Immobilizationmentioning

confidence: 99%

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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“…A confidence level of 90% and a relative standard deviation of 1.4% (n = 12) were obtained [33]. In another study, the development of a flow analysis procedure for the determination of total protein in a bovine blood plasma was carried out using the Biuret method.…”

Section: Spectrophotometric Flow-injection Procedures For Biomedical mentioning

confidence: 99%

Application of Flow-Injection Spectrophotometry to Pharmaceutical and Biomedical Analyses

Costa¹,

Rezende²,

Tavares³

et al. 2017

Spectroscopic Analyses - Developments and Applications

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The discovery of new drugs, especially when many samples have to be analyzed in the minimum of time, demand the improvement or development of new analytical methods. Various techniques may be employed for this purpose. In this context, this chapter gathers the collection of paper and represents the review of past work on spectrophotometric technique coupled to a continuous flow system to determine low concentrations of several chemical species in different kinds of pharmaceutical and biological samples. A short historical background of the flow-injection analysis technique and a brief discussion of the basic principles and potential are presented. Part of this chapter is devoted to describing the sample preparation techniques, principles, and figures of merit of analytical methods. Representative applications of flow-injection spectrophotometry to pharmaceutical and biomedical analysis are also described.

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“…It is a vegetal plant tissue rich in the urease (Luca & Reis, 2001). The jack beans w e r e b e i n g u s e d a s a p o w d e r , w i t h a p a r t i c l e s i z e l e s s t h a n o r 3 m m , i n f r e e f o r m o r immobilized.…”

Section: Biocomponent: Jack Beans (Canavalia Ensiformis)mentioning

confidence: 99%