Singlet Oxygen Luminescence Image in Blood Vessels During Vascular‐Targeted Photodynamic Therapy

Lin, Lisheng; Lin, Huiyun; Shen, Yi; Chen, Defu; Gu, Ying; Wilson, Brian C.; Li, Buhong

doi:10.1111/php.13264

Cited by 11 publications

(4 citation statements)

References 22 publications

(40 reference statements)

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“…Singlet oxygen ( 1 O 2 ) is one of the important indexes for the efficacy of PDT. 48 It has high oxidation activity and ROS to change the spectrum of the detection agent. We selected the 1 O 2 detector ABDA.…”

Section: Methodsmentioning

confidence: 99%

Liposomes loaded with dual clinical photosensitizers for enhanced photodynamic therapy of cervical cancer

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Liposomes loaded with dual clinical photosensitizers for enhanced photodynamic therapy of cervical cancer

et al. 2023

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“…Vascular-targeted photodynamic therapy (V-PDT), which works by inducing vasculature shut-down, is a critical PDT mechanism and has become an important therapeutic method in clinical treatments of vascular-related diseases, including prostate cancer, age-related macular degeneration, and port-wine stains (PWS) [1][2] [3]. Particularly, in China, hematoporphyrin monomethyl ether (HMME)-mediated V-PDT has been proved to be an efficient therapeutic modality for PWS [4] [5].…”

Section: Introductionmentioning

confidence: 99%

High-sensitivity efficacy evaluation methods for vascular-targeted photodynamic therapy of port-wine stains

Xi,

Lin,

Qiu

et al. 2023

6th Optics Young Scientist Summit (OYSS 2023)

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Vascular-targeted photodynamic therapy (V-PDT) has become an important therapeutic method for blood vessel-related diseases such as port-wine stains (PWSs). To achieve more precise V-PDT, quantitative evaluation of V-PDT efficiency appears to be an essential prerequisite. In previous study, methods of Euclidean distance and two-dimensional correlation coefficient, which were calculated based on PWS lesion images, had been employed to evaluate the efficacy of V-PDT. However, results of the mentioned methods show little difference for different V-PDT efficiency, which indicates a limited sensitivity leading to difficulties in V-PDT efficacy evaluation. In this work, evaluation methods with higher sensitivity, including pixel count change, average gray value change, and entropy change which were analyzed based on lesion images before and after V-PDT, were developed for V-PDT efficiency evaluation on PWS. These evaluation methods reveal V-PDT effect from aspects of lesion area, skin color, and skin texture. In addition, relative difference rates of all methods were analyzed for sensitivity comparison. The results indicate that changes of pixel number, average grayscale value, and entropy well reflect differences in V-PDT efficacy, and exhibit much higher sensitivity in comparison with that of Euclidean distance and two-dimensional correlation coefficient, confirming themselves a promising quantitative evaluation method for V-PDT efficacy.

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“…In 1938, Kautsky proposed the energy transfer from a photosensitizer (PS) in its triplet state to molecular oxygen as the generation pathway [3], and it was not until 1968 that Snelling [4] was able to prove it. Today, there is general agreement that local generation of 1 O 2 might be one possible way to treat cancer and other diseases [1,2,[5][6][7]. All it takes is an appropriate PS, illumination at a useful wavelength, and some oxygen [8].…”

Section: Introductionmentioning

confidence: 99%

Singlet Oxygen In Vivo: It Is All about Intensity—Part 2

Hackbarth

Gao

Šubr

et al. 2023

JPM

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Recently, we reported induced anoxia as a limiting factor for photodynamic tumor therapy (PDT). This effect occurs in vivo if the amount of generated singlet oxygen that undergoes chemical reactions with cellular components exceeds the local oxygen supply. The amount of generated singlet oxygen depends mainly on photosensitizer (PS) accumulation, efficiency, and illumination intensity. With illumination intensities above a certain threshold, singlet oxygen is limited to the blood vessel and the nearest vicinity; lower intensities allow singlet oxygen generation also in tissue which is a few cell layers away from the vessels. While all experiments so far were limited to light intensities above this threshold, we report experimental results for intensities at both sides of the threshold for the first time, giving proof for the described model. Using time-resolved optical detection in NIR, we demonstrate characteristic, illumination intensity-dependent changes in signal kinetics of singlet oxygen and photosensitizer phosphorescence in vivo. The described analysis allows for better optimization and coordination of PDT drugs and treatment, as well as new diagnostic methods based on gated PS phosphorescence, for which we report a first in vivo feasibility test.

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Singlet Oxygen Luminescence Image in Blood Vessels During Vascular‐Targeted Photodynamic Therapy

Cited by 11 publications

References 22 publications

Liposomes loaded with dual clinical photosensitizers for enhanced photodynamic therapy of cervical cancer

Liposomes loaded with dual clinical photosensitizers for enhanced photodynamic therapy of cervical cancer

High-sensitivity efficacy evaluation methods for vascular-targeted photodynamic therapy of port-wine stains

Singlet Oxygen In Vivo: It Is All about Intensity—Part 2

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