Singlet oxygen generation by photodynamic agents

Fernandez, Jim M; Bilgin, Mehmet; Grossweiner, Leonard I.

doi:10.1016/s1011-1344(96)07349-6

Cited by 254 publications

(163 citation statements)

References 40 publications

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“…Chlorin e 6 (Ce 6 ) is a second-generation PS with a high quantum yield of singlet oxygen production of 0.65 at pH 7-8 [2]. In addition, the amphiphilic structure of a Ce 6 molecule allows easy penetration through cell membrane, thus ensuring effective accumulation inside cells [3,4].…”

Section: Introductionmentioning

confidence: 99%

Complexes of functionalized quantum dots and chlorin e<sub>6</sub> in photodynamic therapy

Rotomskis¹,

Valančiūnaitė²,

Skripka³

et al. 2013

Lith. J. Phys.

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Recently, it has been suggested that quantum dots (QDs) could be used in the photodynamic therapy of cancer as resonance energy donors for conventional porphyrin type photosensitizers. Here we present the results of the spectroscopic studies on the formation of a non-covalent complex between QDs and photosensitizer chlorin Ce 6 in an aqueous medium and in the presence of bovine serum albumin (BSA). Changes in the absorption and fluorescence spectra of QDs and Ce 6 revealed the formation of a QD-Ce 6 complex which occurs due to hydrophobic interaction between the nonpolar moiety of an amphiphilic photosensitizer and the hydrophobic part of the lipid-based coating of the QD. The photosensitizer conjugated with the QD could be indirectly excited by the Forster resonance energy transfer (FRET) from the QD to Ce 6 . The investigation on the capacity of such complex to generate 1 O 2 showed that the QD-Ce 6 complex irradiated by visible light is able to produce 1 O 2 more efficiently than QDs or Ce 6 taken separately. The photoinactivation of cells incubated with the QD-Ce 6 complex and irradiated in the spectral region where the photosensitizer does not absorb provided evidence that such complex could induce FRET-mediated cell destruction.

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Section: Introductionmentioning

confidence: 99%

Complexes of functionalized quantum dots and chlorin e<sub>6</sub> in photodynamic therapy

Rotomskis¹,

Valančiūnaitė²,

Skripka³

et al. 2013

Lith. J. Phys.

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“…To conduct porphyrin site-directed protein photolysis experiments described in this paper, we chose the porphyrins chlorin e6 and pyropheophorbide-a, compounds that have a λ max of absorption above 600 nm, have significant molar extinction coefficients at these wavelengths and have been used extensively as PS for PDT studies [40][41][42][43][44]. In control experiments, mOGG1 was efficiently photolyzed by these porphyrins at concentrations ranging from 6.4 μM to 64 μM (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

Conlon

Berríos

2007

Journal of Photochemistry and Photobiology B: Biology

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The specific light-induced, non-enzymatic photolysis of mOGG1 by porphyrin-conjugated or rose bengal-conjugated streptavidin and porphyrin-conjugated or rose bengal-conjugated first specific or secondary anti-IgG antibodies is reported. The porphyrin chlorin e6 and rose bengal were conjugated to either streptavidin, rabbit anti-mOGG1 primary specific antibody fractions or goat anti-rabbit IgG secondary antibody fractions. Under our experimental conditions, visible light of wavelengths greater than 600 nm induced the non-enzymatic degradation of mOGG1 when this DNA repair enzyme either directly formed a complex with chlorin e6-conjugated anti-mOGG1 primary specific antibodies or indirectly formed complexes with either streptavidin-chlorin e6 conjugates and biotinylated first specific anti-mOGG1 antibodies or first specific anti-mOGG1antibodies and chlorin e6-conjugated anti-rabbit IgG secondary antibodies. Similar results were obtained when rose bengal was used as photosensitizer instead of chlorine e6. The rate of the photochemical reaction of mOGG1 site-directed by all three chlorine e6 antibody complexes was not affected by the presence of the singlet oxygen scavenger sodium azide. Site-directed photoactivatable probes having the capacity to generate reactive oxygen species (ROS) while destroying the DNA repair system in malignant cells and tumors may represent a powerful strategy to boost selectivity, penetration and efficacy of current photodynamic (PDT) therapy methodologies.

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“…singlet oxygen ( 1 O 2 ) upon light irradiation [14]. ZnPP was also reported to show high singlet oxygen quantum yield [11]. Here, we determined the singlet oxygen from the HA-ZnPP using X-band electron spin resonance (ESR) with TEMPD as singlet oxygen trapping agent.…”

Section: Singlet Oxygen Generation Upon Light Irradiationmentioning

confidence: 99%

“…Though various porphyrin derivatives are utilized for photodynamic therapy in research, the singlet oxygen ( 1 O 2 ) quantum yield of ZnPP (0.91) is higher than other porphyrin derivatives and commercially available photosensitizers, i.e. protoporphyrin IX (0.56), chlorin e6 (0.64) and pheophorbide a (0.69) photofrin (0.89) [11].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid-zinc Protoporphyrin Conjugates for Photodynamic Antitumor Therapy

Eguchi¹,

Nakamura²,

Zhou³

et al. 2017

J Nanomed Nanotechnol

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Singlet oxygen generation by photodynamic agents

Cited by 254 publications

References 40 publications

Complexes of functionalized quantum dots and chlorin e<sub>6</sub> in photodynamic therapy

Complexes of functionalized quantum dots and chlorin e<sub>6</sub> in photodynamic therapy

Site-directed photoproteolysis of 8-oxoguanine DNA glycosylase 1 (OGG1) by specific porphyrin-protein probe conjugates: A strategy to improve the effectiveness of photodynamic therapy for cancer

Hyaluronic Acid-zinc Protoporphyrin Conjugates for Photodynamic Antitumor Therapy

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