Single-walled carbon nanotube based transparent immunosensor for detection of a prostate cancer biomarker osteopontin

Sharma, Abhinav; Hong, Seongkyeol; Singh, Renu; Jang, Jaesung

doi:10.1016/j.aca.2015.02.010

Cited by 57 publications

(15 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional protein detection methods such as ELISA have shown their limit due to a lack of sensitivity and the nature of samples required for the testings. Several studies have focused on the cost-effectiveness and ease of implementing immunosensors for OPN detection demonstrating better sensitivity than ELISA assays, and providing greater potential to develop simple test kits for OPN combined with other protein biomarkers (101–104). …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Human osteopontin: Potential clinical applications in cancer (Review)

Hao

Cui

Owen

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Human osteopontin (OPN) is a glycosylated phosphoprotein which is expressed in a variety of tissues in the body. In recent years, accumulating evidence has indicated that the aberrant expression of OPN is closely associated with tumourigensis, progression and most prominently with metastasis in several tumour types. In this review, we present the current knowledge on the expression profiles of OPN and its main splice variants in human cancers, as well as the potential implications in patient outcome. We also discuss its putative clinical application as a cancer biomarker and as a therapeutic target. IntroductionOsteopontin (OPN) is a bone associated, extracellular matrix glycosylated phosphoprotein which is produced by several cell types, including osteoblasts, osteoclasts, immune cells, endothelial cells, epithelial cells and extra-osseous cells (skin, kidney and lung) (1-3). Due to differences in post-translational modification (PTM) (phosphorylation, glycosylation, sulfation and proteolysis) from different cellular sources, OPN has a molecular weight ranging from 41 to 75 kDa, which may have a cell type-specific structure and function (4-7). OPN plays a major role in various normal physiological processes, including bone remodelling, immune-regulation, inflammation and vascularisation (8,9). In addition, OPN has also been shown to be involved in carcinogenesis with multi-functional activities (10)(11)(12).OPN is involved in a series of biological functions through interactions with different integrins and CD44. Therefore, OPN is classified as a member of the ʻsmall integrin-binding ligand N-linked glycoproteins' (SIBLINGs) together with other molecules, including bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE) (13). Two critical integrin binding sequences of OPN have been identified: arginine-glycine-aspartic acid (RGD) and serine-valine-valinetyrosine-glutamate-leucine-arginine (SVVYGLR). OPN interacts mainly with various αv (particularly αvβ1, αvβ3, αvβ5) integrin receptors via the classical RGD sequence, and interacts with α9β1, α4β1, α4β7 via SVVYGLR (14-16). In addition, it also interacts with the CD44 splice variants, CD44v3, CD44v6 and CD44v7, via the C-terminal fragment calcium binding site (17)(18)(19)(20). These properties of OPN induce the activation of signal transduction pathways, leading to cell proliferation, adhesion, invasion and migration, which have been demonstrated by both in vitro and in vivo models (21-23). The binding of OPN to integrins and CD44 initiates a downstream signalling cascade via the PI3K/AKT signalling pathway leading to NF-κB mediated cell proliferation and survival (24)(25)(26). In additon, through the Ras/Raf/MEK/ERK signalling pathway, an OPN-integrin complex and subsequent induction of AP-1-dependent gene expression, urokinase-type plasminogen activator (uPA) and matrix metalloproteinases (MMPs) confer a metastatic phenotype on some cancer cell types (27)(28)(29...

show abstract

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Human osteopontin: Potential clinical applications in cancer (Review)

Hao

Cui

Owen

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…Figures 1(b were aligned between the two Cr/Au electrodes after DEP and that these aligned SWCNTs differed from the non-aligned and dense network of SWCNTs formed through sedimentation (Sharma et al, 2015;Singh et al, 2014). The AFM images reveal that SWCNTs were deposited at a high density between the detection electrodes (Cr/Au) (Fig.…”

Section: Fe-sem and Afm Analysesmentioning

confidence: 92%

Rapid electrical immunoassay of the cardiac biomarker troponin I through dielectrophoretic concentration using imbedded electrodes

Sharma

Han

Jang

2016

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

“…Sharma et al . have reported their work on fabrication of an osteopontin‐based immunosensor . There has been a substantial interest in osteopontin as a biomarker of cancer aggression .…”

Section: Disease Biomarker Detection At Structurally Small Electrodesmentioning

confidence: 99%

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

2017

View full text Add to dashboard Cite

This Minireview focuses on recent advances in the applications of microelectrodes to detect and monitor targeted analytes in bioelectrochemical processes. Notably, these processes are electrochemically driven reactions that involve the detection of targets from the biological realm. Wide‐ranging applications of electrochemical sensors have been reported in the last few decades in various research fields, owing to favorable attributes such as high selectivity and sensitivity, rapid analysis, simplicity, easy fabrication, and cost effectiveness. Accordingly, in this Minireview, we explore recent advances in bioelectrochemistry based on small detection probes or structures modified for a variety of analytes, exploiting multi‐approach advantages of enhanced electrochemical detection surface or targeted analyte pursuit. The target analytes included in this Minireview are neurotransmitters and disease biomarkers detected using enzymatic and non‐enzymatic electrode modifications.

show abstract

Single-walled carbon nanotube based transparent immunosensor for detection of a prostate cancer biomarker osteopontin

Cited by 57 publications

References 28 publications

Human osteopontin: Potential clinical applications in cancer (Review)

Human osteopontin: Potential clinical applications in cancer (Review)

Rapid electrical immunoassay of the cardiac biomarker troponin I through dielectrophoretic concentration using imbedded electrodes

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

Contact Info

Product

Resources

About