Single-stranded endonuclease activity in the 
excretory–secretory products of <i>Trichinella spiralis</i> and 
<i>Trichinella pseudospiralis</i>

Mak, C. H.; Chung, Y. Y. Y.; Ko, Ronald C.

doi:10.1017/s0031182099005879

Cited by 13 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The endonuclease activity is associated with at least three molecular forms, designated approximately as 25, 30, and 58 kDa, respectively (Mak and Ko 1999). Unlike the double-stranded endonuclease, the single-stranded molecule is divalent cation independent and is present in both T. spiralis and T. pseudospiralis E-S products (Mak et al 2000). The single-and double-stranded endonucleases are likely to be encoded by different proteins and may have different functions.…”

Section: Endonucleasesmentioning

confidence: 99%

Functional genes and proteins of Trichinella spp.

Nagano

Takahashi

2008

Parasitol Res

View full text Add to dashboard Cite

Research of Trichinella proteins has been conducted with emphasis on excretory-secretory (E-S) products of muscle larvae because of two reasons. The first is that it has prominent and narrow specific antigenicity, and the second is that it may play some role in nurse cell formation after being secreted into host muscle cells. Proteomic analysis of E-S proteins was further advanced by the aid of new analytical methods such as gene cloning, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, and expressed sequence tags database analysis. As the research progressed, the interest of researchers moved to identification of function of E-S products, which has shed further light on the intriguing relationships between parasites and hosts. Major constituents of the E-S products include 43-, 53-, and 45-kDa glycoprotein derived from the stichosome. Many proteins were discovered in E-S products after the 43-, 53-, and 45-kDa proteins although the relationships among them remain unclear. Some of the new proteins were partially defined in terms of their function including nuclear antigens, MyoD-like protein, TsJ5 protein, etc. There are better-characterized proteins based on the gene molecular method, which allow easier identification of the function of proteins of interest. Such examples were demonstrated by proteinases, proteinase inhibitors, heat shock proteins, glycosidases, etc.

show abstract

Section: Endonucleasesmentioning

confidence: 99%

Functional genes and proteins of Trichinella spp.

Nagano

Takahashi

2008

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…3, 2004 GENETICS of lipid peroxidation products (malonic dialdehyde and conjugated dienes) and decrease in activity of antioxidant enzymes (superoxide dismutase and catalase) [3]. Moreover, secretory and excretory products of trichinella larvae include single-chain and two-chain endonucleases [8,9] and DNA-twisting proteins [10].…”

Section: Resultsmentioning

confidence: 99%

Damage to cell DNA in the bone marrow and testes of mice with experimental trichinosis

Bekish¹,

Дурнев

2004

Bull Exp Biol Med

View full text Add to dashboard Cite

Metabolites of Trichinella spiralis produced genotoxic and cytotoxic effects on somatic and generative cells of the host organism. They increased the number of single-chain breaks, alkaline-labile sites in nuclear DNA, and count of apoptotic cells in the bone marrow and testes of infected mice. These effects depended on the stage of parasite development in the host organism and became more pronounced with increasing invasion intensity.

show abstract

“…These differences in host responses are likely due to the differences of excretory-secretory products, especially stichosomal excretion granules which are introduced in the cytoplasm of infected muscle cells. Cloning and characterization of stichosomal protein are in progress at some laboratories, which resulted in the discovery of functional proteins including DNase (Mak and Ko 1999;Mak et al 2000), serine protease (Nagano et al 2003), and the proteinase inhibitor . We have already cloned 43 and 53 kDa stichosomal proteins of both T. spiralis and T. pseudospiralis.…”

Section: Discussionmentioning

confidence: 99%

Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection

Boonmars

Nagano

et al. 2005

Parasitol Res

View full text Add to dashboard Cite

A time course study was performed to reveal the sequence of histopathology after Trichinella spiralis or T. pseudospiralis infection in mice. A cyst was formed in the former case by about 18 days post infection and prominent myopathy was restricted within the cyst. In the latter case, however, no typical cyst was formed, and myopathy spread diffusely over the infected muscle tissues occupying half the area of muscle sections. An electron microscope observation revealed that the disintegration of muscle cells was delayed in T. pseudospiralis infection than in T. spiralis infection. Quantitative reverse transcription polymerase chain reaction (RT-PCR) showed that apoptosis-related genes were expressed for a longer term in muscles infected with T. pseudospiralis than in those with T. spiralis, although the same spectrum of genes are mobilized. Examined apoptosis-related genes included tumor suppressor genes p53, p53; mouse double minute 2, MDM2; cyclin-dependent kinase inhibitor p21 (WAF1), p21(waf) ; Bcl-2 associated protein X, BAX; apoptotic protease activating factor 1, Apaf-1; Caspase 9 and serine/ threonine protein kinase, PKB. Micro-dissection of the infected muscle tissue and subsequent RT-PCR confirmed that the expressions of these genes are restricted to tissue with myopathy. Thus, the expression of the apoptosis-related genes correlated with continuous and diffuse myopathy caused by T. pseudospiralis infection.

show abstract

Single-stranded endonuclease activity in the excretory–secretory products of Trichinella spiralis and Trichinella pseudospiralis

Cited by 13 publications

References 13 publications

Functional genes and proteins of Trichinella spp.

Functional genes and proteins of Trichinella spp.

Damage to cell DNA in the bone marrow and testes of mice with experimental trichinosis

Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection

Contact Info

Product

Resources

About