Single-strand breaks in Escherichia coli DNA caused by treatment with nitrosoguanidine

Olson, A.O.; Baird, K.M.

doi:10.1016/0005-2787(69)90063-x

Cited by 36 publications

(8 citation statements)

References 6 publications

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“…The effect of caffeine on the premutationM lesions produced by NG is not clear, however Olson and Baird (1969) have shown that NG produces single strand breaks in the DNA of E. coli which are repaired: it is possible to assume that such breaks are sensitive to the recombinational repair processes responsible for the induction of mutation and that such a fraction of the damage induced by NG is caffeine-sensitive. In Saccharomyces cerevisiae, moreover, ZimmermaIm et al (1966) have shown that NG is an efficient compound in inducing mitotic recombination as well as mutation.…”

Section: Discussionmentioning

confidence: 97%

On the effect of caffeine on mutation and recombination in Schizosaccharomyces pombe

Loprieno

Schüpbach

1971

Molec. Gen. Genet.

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Summary. In Schizosaccharomyces pombe the experiments performed in order to assess some possible influence of caffeine on biological processes related to the induced or spontaneous mutations and meiotic recombinations, have shown that caffeine decreases UV-and NGinduced forward mutations at ad-6 and ad-7 loci and UV-induced reverse-mutations of hismutants whereas spontaneous mutations to adenine or histidine independence were not affected; intergenic meiotic recombinations in the lVlT chromosomal region were also decreased when caffeine was present in the crossing medium.

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Section: Discussionmentioning

confidence: 97%

On the effect of caffeine on mutation and recombination in Schizosaccharomyces pombe

Loprieno

Schüpbach

1971

Molec. Gen. Genet.

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“…To enhance the effectiveness of CUSO4 5H20 treatment, we first treated the K. pneumoniae cells with NTG. NTG is known to cause single-stranded breaks of the DNA in bacterial cells through alkylation of guanine and adenine (16,19).…”

Section: Methodsmentioning

confidence: 99%

“…The original cells of K. pneumoniae were treated for 45 min with 400 ppm (p,g/ml) of NTG (Fluka AG, Buchs, Switzerland) (19). The treated cells were grown for three consecutive generations in NTG-free D-xylose growth medium containing yeast extract (5.0 g liter-'), malt extract (5.0 g liter-'), peptone (10.0 g liter-1), and D-xylose (20.0 g liter -').…”

Section: Methodsmentioning

confidence: 99%

Kinetics of ethanolic fermentation of D-xylose by Klebsiella pneumoniae and its mutants

Banerjee¹

1989

Appl Environ Microbiol

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The microbial production of ethanol from D-xylose by a new soil isolate of Klebsiella pneumoniae and the mutants K. pneumoniae MB-16 and MB-16-1048 was studied. Kinetic and physiological properties of the mutants were compared with those of the original isolate. The volumetric rates of ethanol formation by mutants MB-16-1048 and MB-16 and the original isolate were 1.58, 0.50, and 0.06 g liter-' h-1, respectively. The cultivation times of mutants MB-16-1048 and MB-16 were 20 and 18 h, respectively, and that of the original isolate was 118 h. Both the mutants exhibited metabolic similarities with the original isolate. Ethanol was the major end product of fermentation in all three strains. Acetic acid and carbon dioxide were the other two important by-products of fermentation. Pyruvic acid was accumulated in significant proportions as an intermediate. The proportion of pyruvate in the original isolate was 54% of the total D-xylose utilized, whereas for MB-16 and MB-16-1048 the values were about 42 and 22%, respectively. The lower fractions of pyruvate in mutants MB-16 and MB-16-1048 showed up as a 41 and 82% improvement, respectively, over the original isolate in terms of the ethanol yield.

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“…The data from X-irradiation can be directly compared to that for NG sensitivity; NG is a monofunctional alkylating agent which is also known to produce single-strand breaks in DNA (Olson and Baird, 1969), and recombina tion-deficient mutants are uniquely sensitive to NG (Witkin, 1969b). Fig ure 6 indicates that, as with X-irradiation, only mutant UVS-1 is rapidly inactivated by NG while the remaining mutants are as NG resistant as strain 112 (Table 11) (Table 7), it is important to note that UVS-1 is totally unable to act as a recipient of the chromosomal pase marker while still expressing the penicillinase plasmid.…”

Section: Estimation Of Non-viable Fraction Of Cell Populationsmentioning

confidence: 99%

A characterization of mutants of Staphylococcus aureus exhibiting increased sensitivity to ultraviolet radiation

Goering

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Single-strand breaks in Escherichia coli DNA caused by treatment with nitrosoguanidine

Cited by 36 publications

References 6 publications

On the effect of caffeine on mutation and recombination in Schizosaccharomyces pombe

On the effect of caffeine on mutation and recombination in Schizosaccharomyces pombe

Kinetics of ethanolic fermentation of D-xylose by Klebsiella pneumoniae and its mutants

A characterization of mutants of Staphylococcus aureus exhibiting increased sensitivity to ultraviolet radiation

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