Single step determination of fragrances in Cucurbita flowers by coupling headspace solid-phase microextraction low-pressure gas chromatography–tandem mass spectrometry

Granero, A. M.; González, Francisco Javier Egea; Frenich, Antonia Garrido; Sanz, J.F.; Vidal, José Luis Martı́nez

doi:10.1016/j.chroma.2004.06.026

Cited by 25 publications

(10 citation statements)

References 35 publications

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“…Plants are monoecious with separate male and female flowers that last for 1 day, opening before sunrise and wilting by early afternoon (McGregor 1976). The scent of male and female Cucurbita blossoms attracts Acalymma vittatum Fabricius (striped cucumber beetle, Chrysomelidae) (Andersen and Metcalf 1986;Lewis et al 1990;Metcalf and Lampman 1991;Metcalf et al 1995;Granero et al 2004;Ferrari et al 2006), a specialist herbivore that is responsible for extensive damage to northeastern cucurbit crops (Hoffmann et al 1996). Leaves and roots of some Cucurbita species also produce volatiles that may attract cucumber beetles and other insects (Peterson et al 1994;Cosse and Baker 1999).…”

Section: Methodsmentioning

confidence: 99%

Pollinator and Herbivore Attraction to Cucurbita Floral Volatiles

2007

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Mutualists and antagonists may place conflicting selection pressures on plant traits. For example, the evolution of floral traits is typically studied in the context of attracting pollinators, but traits may incur fitness costs if they are also attractive to antagonists. Striped cucumber beetles (Acalymma vittatum) feed on cucurbits and are attracted to several volatiles emitted by Cucurbita blossoms. However, the effect of these volatiles on pollinator attraction is unknown. Our goal was to determine whether pollinators were attracted to the same or different floral volatiles as herbivorous cucumber beetles. We tested three volatiles previously found to attract cucumber beetles in a factorial design to determine attraction of squash bees (Peponapis pruinosa), the specialist pollinators of cucurbita species, as well as the specialist herbivore A. vittatum. We found that 1,2,4-trimethoxybenzene was attractive to both the pollinator and the herbivore, indole was attractive only to the herbivore, and (E)-cinnamaldehyde was attractive only to the pollinator. There were no interactions among volatiles on attraction of squash bees or cucumber beetles. Our results suggest that reduced indole emission could benefit plants by reducing herbivore attraction without loss of pollination, and that 1,2,4-trimethoxybenzene might be under conflicting selection pressure from mutualists and antagonists. By examining the attraction of both mutualists and antagonists to Cucurbita floral volatiles, we have demonstrated the potential for some compounds to influence only one type of interaction, while others may affect both interactions and possibly result in tradeoffs. These results shed light on the potential evolution of fragrance in native Cucurbita, and may have consequences for yield in agricultural settings.

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Section: Methodsmentioning

confidence: 99%

Pollinator and Herbivore Attraction to Cucurbita Floral Volatiles

2007

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“…Because blends of plant volatiles can consist of many chemically diverse compounds as well as isomers, a simple GC‐MS analysis run might not be sufficient for the identification of all products. Tandem mass spectrometry (MS‐MS) has been established allowing separate analyses of single compounds of complex GC peaks (Granero et al. , 2004; Ragunathan et al.…”

Section: Gas Chromatographic Separation and Detection Of Plant Vocsmentioning

confidence: 99%

Practical approaches to plant volatile analysis

et al. 2006

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SummaryPlants emit volatile organic compounds (VOCs) that play important roles in their interaction with the environment and have a major impact on atmospheric chemistry. The development of static and dynamic techniques for headspace collection of volatiles in combination with gas chromatography-mass spectrometry analysis has significantly improved our understanding of the biosynthesis and ecology of plant VOCs. Advances in automated analysis of VOCs have allowed the monitoring of fast changes in VOC emissions and facilitated in vivo studies of VOC biosynthesis. This review presents an overview of methods for the analysis of plant VOCs, including their advantages and disadvantages, with a focus on the latest technical developments. It provides guidance on how to select appropriate instrumentation and protocols for biochemical, physiological and ecologically relevant applications. These include headspace analyses of plant VOCs emitted by the whole organism, organs or enzymes as well as advanced on-line analysis methods for simultaneous measurements of VOC emissions with other physiological parameters.

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“…The performance of the method was assessed calculating linear ranges, recovery rates, precision and lower limits (Mena Granero et al 2004). For calibration purposes, three empty boxes with the same dimensions as the nest box previously described were spiked with appropriate volumes of the 20 mg l −1 standard mixture containing all the analytes.…”

Section: Laboratory Trial Design For Pheromone Analysismentioning

confidence: 99%

Chemical compounds of the foraging recruitment pheromone in bumblebees

Granero

Sanz

González

et al. 2005

Naturwissenschaften

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When the frenzied and irregular foodrecruitment dances of bumblebees were first discovered, it was thought that they might represent an evolutionary prototype to the honeybee waggle dance. It later emerged that the primary function of the bumblebee dance was the distribution of an alerting pheromone. Here, we identify the chemical compounds of the bumblebee recruitment pheromone and their behaviour effects. The presence of two monoterpenes and one sesquiterpene (eucalyptol, ocimene and farnesol) in the nest airspace and in the tergal glands increases strongly during foraging. Of these, eucalyptol has the strongest recruitment effect when a bee nest is experimentally exposed to it. Since honeybees use terpenes for marking food sources rather than recruiting foragers inside the nest, this suggests independent evolutionary roots of food recruitment in these two groups of bees.

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Single step determination of fragrances in Cucurbita flowers by coupling headspace solid-phase microextraction low-pressure gas chromatography–tandem mass spectrometry

Cited by 25 publications

References 35 publications

Pollinator and Herbivore Attraction to Cucurbita Floral Volatiles

Pollinator and Herbivore Attraction to Cucurbita Floral Volatiles

Practical approaches to plant volatile analysis

Chemical compounds of the foraging recruitment pheromone in bumblebees

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