Single short-term signal that enhances cooperative activity of old rat hepatocytes acts for several days

Brodsky, V. Ya.; Zvezdina, N. D.; Nechaeva, N. V.; Novikova, T. E.; Gvasava, Inessa G.; Fateeva, V. I.; Мальченко, Л. А.

doi:10.1016/j.cellbi.2005.06.002

Cited by 9 publications

(7 citation statements)

References 16 publications

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“…cooperation of cells in the formation of a common rhythm in a cell population. The main data have been published during the last five years (Brodsky et al, a, b, 2004(Brodsky et al, , 2005Zvezdina et al, 2000). The ultradian rhythm of protein synthesis was used as a marker of cell interactions and synchronisation of oscillations in rat hepatocytes cultured on slides.…”

Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

“…6). Addition of blood serum of three-month-old rats to serum-free basal medium of non-synchronous sparse hepatocyte cultures Brodsky et al (2000Brodsky et al ( , 2003aBrodsky et al ( , b, 2004Brodsky et al ( , 2005 ; Zvezdina et al (2000). 1 PPPP -d-l-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol-HCl -inhibitor of ganglioside synthesis and shedding (Li & Ladisch, 1996, 1997Kong et al, 1998).…”

Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

“…Enhanced cooperative activity of hepatocytes initiated by signal factors such as bovine brain gangliosides (BBG) or phenylephrine was retained for at least 48 h (Brodsky et al, 2005). BBG were present in the medium 30 min before detection of a protein synthesis rhythm in dense cultures of an old rat.…”

Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

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Direct cell–cell communication: a new approach derived from recent data on the nature and self-organisation of ultradian (circahoralian) intracellular rhythms

Brodsky

2005

Biol. Rev.

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Recent data concerning ultradian (circahoralian) intracellular rhythms are used to assess the biochemical mechanisms of direct cell-cell communication. New results and theoretical considerations suggest a fractal nature of ultradian rhythms and their self-organisation. The fundamental and innate nature of these rhythms relates to their self-similarity at different levels of cell and tissue organisation. They can be detected in cell-free systems as well as in cells and organs in vivo. Such rhythms are a means of finding an optimal state of cell function rather than achieving a state of absolute stability. As a consequence, oscillations, being irregular and numerous by the set of periods, are resilient to functional overload and injury. Recent data on the maintenance of their fractal structure and, especially on the selection of optimal periods are discussed. The positive role of chaotic dynamics is stressed. The ultradian rhythm of protein synthesis in hepatocytes in vitro was used as a marker of direct cell-cell communication. The system demonstrates cell cooperation and synchronisation throughout the cell population, and suggests that the ultradian rhythms are self-organised. These observations also led to the detection of mechanisms of direct cell-cell communication in which extracellular factors have an essential role. Experimental evidence indicated the involvement of gangliosides and/or catecholamines in this large-scale synchronisation of protein synthesis. The response of all, or a major part, of the cell population is important; after the initial trigger effect, a periodic pattern is retained for some time. The influence of Ca2+-dependent protein kinases on protein phosphorylation can be a final step in the phase modulation of rhythms during cell-cell synchronisation. The intercellular medium plays an important role in self-synchronisation of ultradian rhythms between individual cells. Low cooperative activity of hepatocytes of old rats resulted from altered composition of the intercellular medium rather than direct effects of animal and cellular ageing. Similarly, in the whole body, changes in levels of gangliosides and catecholamines in the blood serum, a natural intercellular medium, can be critical events in age-dependent changes of the serum and accordingly cell-cell synchronisation. Hepatocytes of old rats exhibit some of the properties of young cells following an increase in blood serum ganglioside level, as well as, in in vitro conditions, after the addition of gangliosides to the culture medium. Together with data on ultradian functional and metabolic rhythms, all the material reviewed here allows us to propose a mechanism of direct cell-cell cooperation via the medium in which the cells exist, that supplements the nervous and hormonal central regulation of organ functions. Ultradian intracellular rhythms may thus provide a finer framework within which the integrated dynamics of respiration, heart rate, brain activity, and even behavioural patterns, are brought to an optimal functional pattern. I...

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Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

Section: Cell-cell Cooperation In Oscillatory Behaviour Of Cell mentioning

confidence: 99%

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Direct cell–cell communication: a new approach derived from recent data on the nature and self-organisation of ultradian (circahoralian) intracellular rhythms

Brodsky

2005

Biol. Rev.

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“…Ultradian rhythms are an innate cell property presumably of a fractal nature (Barnsley et al, 1987;Brodsky, 1992Brodsky, , 2006Lloyd, 1992Lloyd, , 2005Lloyd, 1993, 1995). In a series of articles already published in this journal (Brodsky et al, 2000(Brodsky et al, , 2003a(Brodsky et al, ,b, 2004(Brodsky et al, , 2005, some signal factors that promoted synchronization of protein synthesis rhythm were described. Depending on cell density, two types of primary hepatocyte cultures on slides in the serum-free medium were obtained, dense cultures with closely arranged cells, and sparse cultures with widely separated cells.…”

Section: Introductionmentioning

confidence: 99%

“…It was clear that experimental synchronization of sparse cultures as well as desynchronization of dense cultures could be considered as actual events for the analysis of cell self-organization. In a series of articles already published in this journal (Brodsky et al, 2000(Brodsky et al, , 2003a(Brodsky et al, ,b, 2004(Brodsky et al, , 2005, some signal factors that promoted synchronization of protein synthesis rhythm were described. The cells responded to either ganglioside or catecholamine signaling with an increase of calcium ions in the cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

Involvement of protein kinases in self‐organization of the rhythm of protein synthesis by direct cell—cell communication

Brodsky

Zvezdina

Fateeva

et al. 2007

Cell Biology International

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Primary cultures of rat hepatocytes grown on slides were studied in serum-free medium. Ultradian protein synthesis rhythm was used as a marker of synchronization of individual oscillations, resulting in the formation of a common rhythm of the cell population, i.e. cell-cell self-organization. Dense synchronous and sparse non-synchronous cultures were used to estimate effect of protein kinase activity on the kinetics of protein synthesis. Treatment of dense cultures with the inhibitors H7 (40 microM) or H8 (25 microM) resulted in a loss of the protein synthesis rhythm, a suppression of the cell-cell self-organization. Stimulation of protein kinase activity with either 0.5 or 1.0 microM phorbol 12-miristate-13-acetate (PMA) or 10 microM forskolin caused the appearance of the synthetic rhythm in non-synchronous sparse cultures under otherwise normal conditions. Inhibition of protein kinase activity with H7 resulted in signal factors, such as gangliosides and phenylephrine, failing to initiate this rhythm in sparse cultures. Activation of protein kinase activity with PMA shifted the phase pattern of the protein synthesis rhythm. Thus, according to our previous and the new data, protein kinase activity and consequently protein phosphorylation is the crucial step of sequence of processes resulting in synchronization during self-organization of cells in producing a common rhythm in the population. The general pathway can be presented as follows: signaling of gangliosides or other calcium agonists-->efflux of calcium ion from intracellular stores, with elevation of calcium concentration in the cytoplasm-->activation of protein kinases-->protein phosphorylation-->synchronization of individual oscillations in protein synthesis rates-->induction of a common rhythm throughout this population. The data have been discussed concerning similarity of the direct cell-cell communication and the cell self-organization in cultures and in organism.

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Self-Organized Intracellular Ultradian Rhythms Provide Direct Cell-Cell Communication

Brodsky

Lloyd

2008

Ultradian Rhythms From Molecules to Mind

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Single short-term signal that enhances cooperative activity of old rat hepatocytes acts for several days

Cited by 9 publications

References 16 publications

Direct cell–cell communication: a new approach derived from recent data on the nature and self-organisation of ultradian (circahoralian) intracellular rhythms

Direct cell–cell communication: a new approach derived from recent data on the nature and self-organisation of ultradian (circahoralian) intracellular rhythms

Involvement of protein kinases in self‐organization of the rhythm of protein synthesis by direct cell—cell communication

Self-Organized Intracellular Ultradian Rhythms Provide Direct Cell-Cell Communication

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