Single Nucleotide Polymorphisms in IL-10, IL-12p40, and IL-13 Genes and Susceptibility to Glioma

Abstract

Glioma is one of the most aggressive and most common tumors of the central nervous system (CNS) in humans. The exact causes of glioma are not well known, but evidence suggests the involvement of genetic factors in addition to environmental risk factors. The present study aimed to determine whether polymorphisms in IL-10-1082A/G, IL-12p40 1188C/A, and IL-13+2044G/A (rs20541) are associated with the incidence of glioma in Iraqi patients. Ninety-six patients with different grades of glioma and 40 apparently healthy individuals were recruited. A blood sample and genomic DNA were collected from all subjects. The amplification refractory mutation system and sequence-specific primer polymerase chain reaction (PCR) were used for genotyping of IL-10-1082A/G and IL-12p40 1188C/A, respectively; whereas, the IL-13+2044G/A was detected by DNA sequencing after amplification of the genes by PCR.All SNPs were within Hardy-Weinberg equilibrium and each appeared in three genotypes in patients and controls. In IL-10-1082A/G, these genotypes frequencies were AA (75%), AG (22.93%) and GG (2.07%) in patients as compared to similar frequencies (62.5%), (27.5%) and (10%) respectively, in controls. The variant IL-12p40 1188C/A genotype was AA (72.92%), AC (23.96%), and CC (3.13%%) in patients as compared to 65%, 30%, and 5%, respectively, in controls. The frequencies of IL-13+2044G/A genotypes (GG, GA, and AA) were 89.58%, 9.37%, and 1.04% among patients versus 47.5%, 32.5% and 20%, respectively, among controls. These results suggest a protective role of mutant alleles G and A in IL-10-1082A/G and IL-13+2044G/A against gliomas. Further studies with more rigorous parameter designs will be needed to confirm the current findings.

IL-10 and PRKDC polymorphisms are associated with glioma patient survival

“…Ours was the first study to associate the IL-10 rs1800871 C/T genotype with improved survival in low-grade glioma patients. IL-10 gene polymorphisms have been associated with multiple cancers, such as gastric [ 18 ], breast [ 19 ], and non-small cell lung cancer [ 20 ], as well as gliomas [ 9 , 21 ]. Inflammation plays roles in all phases of tumor development, and IL-10, a well-known immuno-modulatory cytokine, may provide a functional link between inflammation and cancer [ 22 ].…”

Assessing the association between EFEMP1 rs3791679 polymorphism and risk of glioma in a Chinese Han population

“…Genetic polymorphisms and somatic mutations in factors such as interleukin-8 (IL-8), IL-10, IL-12p40, IL-13, XRCC1, ERCC2, and VEGFR2 are associated with the risk of glioma (Jia et al, 2015;Li et al, 2015;Liu et al, 2015;Shamran et al, 2015;Wang et al, 2015;Xu et al, 2015). Multiple-gene alterations can induce genetic and molecular aberrations, which can also lead to the pathogenesis of glioma.…”

Association between <b><i>IL-13</i></b> Gene rs20541 Polymorphism and Glioma Susceptibility: A Meta-Analysis

“…Conclusion: There may be a weak association between the IL-13 gene rs20541 polymorphism and glioma susceptibility, and the associations may be different between ethnicities. 16,20]. As such, this meta-analysis was performed in order to give precise estimations of the relationship between the IL-13 gene rs20541 polymorphism and glioma susceptibility.…”

“…The results would become statistically insignificant for rs20541 polymorphism and glioma susceptibility when the study by Backes et al [14] or Wiemels et al [28] was omitted in the dominant model (AA + AG vs. GG). Moreover, when we remove the study by Shamran et al [20], heterogeneity was significantly reduced in all comparison models.…”