Single nucleotide polymorphisms in CAPN and leptin genes associated with meat color and tenderness in Nellore cattle

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ABSTRACT. Given the important role of leptin in metabolism, we looked for a possible association of leptin and leptin receptor polymorphisms with carcass and growth traits in Nellore cattle. We examined associations of leptin and leptin receptor SNPs with ultrasound carcass (longissimus dorsi muscle area (ribeye area), backfat thickness and rump fat thickness and growth traits (weaning weight adjusted to 210 days of age, yearling weight adjusted to 550 days of age, weight gain of weaning to yearling and scrotal circumference adjusted to 550 days of age) of 2162 Bos primigenius indicus (Nellore) animals. Allele and genotypic frequencies were calculated for each marker. Allele substitution, additive and dominance effects of the polymorphisms were also evaluated. Some alleles of the molecular markers had low frequencies, lower than 1%, in the sample analyzed, although the same polymorphisms described for B. p. taurus cattle were found. Due to very low allelic frequencies, the E2JW, A59V and UASMS2 markers were not included in the analysis, because they were almost fixed. E2FB was found to be significantly associated with weight gain, ribeye area and backfat thickness. The promoter region markers, C963T and UASMS1, were also found to be significantly associated with ribeye area. T945M was significantly associated with weight gain. We conclude that the leptin and receptor gene markers would be useful for marker-assisted selection.

Section: Association Analysiscontrasting

confidence: 43%

Association of single nucleotide polymorphisms in the bovine leptin and leptin receptor genes with growth and ultrasound carcass traits in Nellore cattle

Silva

Ferraz

Meirelles³

et al. 2012

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“…UoGCAST and CAPN4751 seemed to be the most promising markers for meat tenderness in this breed, but their effects on other productive traits, such as feed intake and feed efficiency, have not yet been established. In the current study, the variability for CAPN4751 and UoGCAST in Nellore cattle, previously reported in other studies (Pinto et al, 2010(Pinto et al, , 2011, was confirmed, showing that the allelic and genotypic frequencies seem consistent across Nellore populations. Similarly, the cited authors obtained low frequencies for the CC type of CAPN4751 (3.2%) and well-distributed frequencies across the three genotypes of UoGCAST in agreement with studies in Brahman cattle (White et al, 2005;Casas et al, 2006).…”

Section: Discussionsupporting

confidence: 89%

Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle

Gomes¹,

Silva²,

Carvalho³

et al. 2013

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ABSTRACT.We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, residual feed intake (RFI), ultrasound backfat, rump fat, and ribeye area. Genotypes were obtained for SNPs in the growth hormone receptor (GHR-1 and GHR-2); calpain (CAPN4751); calpastatin (UoGCAST); ubiquitin-conjugating enzyme 2I (UBE2I-1 and UBE2I-2); R3H domain containing 1 (R3HDM1-1, -2, -3, and -4), ring finger protein 19 (RNF19); proteasome 26S subunit, non-ATPase, 13 (PSMD13); ribosomal protein, large, P2 (RPLP2); and isoleucine-tRNA synthetase 2, mitochondrial (IARS2) genes. Allelic substitution, additive and dominant effects were tested and molecular breeding values were computed. CAPN4751, GHR-1 and -2, IARS2, R3HDM1-4, and UoGCAST were found to be normally segregating polymorphisms. Additive and dominance effects were observed on BWG, feed efficiency and carcass traits, although dominant effects predominated. Significant allelic substitution effects were observed for CAPN4751, GHR-1 and -2, and UoGCAST on BWG, gross feed efficiency, RFI, and carcass traits, under single-or multiple-marker analyses. Correlations between molecular breeding values and phenotypes were low, excepted for RFI, based on allelic substitution estimates obtained by stepwise linear regression. We conclude that SNPs in genes related to protein turnover are related to economically important traits in Nellore cattle.

“…Curi et al (2009) evaluated animals of different breeds (Nellore, Angus x Nellore, Rubia Galega x Nellore, Canchim, Brangus three-way cross, and Braunvieh three-way cross) and observed differences in meat tenderness between animals with CT and TT genotypes; however, animals with the CC genotype were not used in the population analyzed by those authors. Additionally, Pinto et al (2011) observed that additive effects of alleles for the CC genotype of this marker may be associated with a less intense red color. Therefore, important information on this genotype should also be considered.…”

Section: Effects Of Evaluated Snp Markersmentioning

confidence: 96%

Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle

Carvalho

Eler

Bonin³

et al. 2017

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ABSTRACT. The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the µ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21 for the marker CAPN4753, and at days 14 and 21 for the marker UOGCAST1. For genotypic combinations of markers, the results were significant for the combination CAPN4751/UOGCAST1 in the three maturation periods and CAPN4753/UOGCAST1 at days 14 and 21 of maturation.