Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls

Abstract

Selection of high fertile bulls with the help of marker assisted selection has gained importance in recent years. The low heritability of fertility traits hampers improvement of these traits by conventional selection based on phenotypic records. No information is available on the role of SNPs in KiSS1 gene in cattle on semen quality parameters in bovines. KiSS1 genes code for Kisspeptin, which are essential upstream regulators of neurons secreting gonadotropin-releasing hormone and play crucial role in reproduction.The coding regions along with exon-intron boundaries of KiSS1 gene, was characterized using PCR-SSCP method and direct sequencing. Two genotypes were observed which were represented as SSCP pattern 1 and pattern 2 and found to carry one SNPs (T153C) and one insertion of G at 291_292bp. The bulls with pattern 2 were heterozygous with respect to the transition T153C and pattern1 bulls were homozygous with TT genotype. The transition was predicted to cause amino acid change from Valine to Alanine. The frequency of bulls with pattern1 and pattern 2 were 0.67 and 0.33 in 67 Holstein Friesian bulls and 0.73 and 0.27 in 13 Khillari bulls. The association study of genotypes with semen quality parameters revealed significant association of genotypes with acrosome integrity in fresh semen (P<0.05) and no association with sperm concentration, volume per ejaculate, percent live sperm and Hypo Osmotic Swelling Test (HOST) with higher acrosome integrity in bulls with pattern2. Upon validation of the results in larger population and identifying the exact role of the novel SNP T153C and insertion of G at 291_292bp, they could be incorporated in selection programme for improving fertility in bulls.as markers for acrosome integrity in cattle.

Association of SNP T125A on KiSS1 gene with reproduction hormone levels in Kaligesing goat

“…Cao et al (2010) revealed that SNP G296C in Jining goat showed association with litter size. Genotype CC had kids more than genotype GC or In cattle, Divya et al (2017) found that SNP T153C and insertion G at 291_292 bp in KiSS 1 gene in bulls were correlated with acrosome integrity in fresh semen. Cao et al (2019) and Lapatto et al (2007) reported that the secretion of GnRH-dependent luteinizing hormone (LH) and follicle stimulating hormone (FSH) in a variety of mammalian spesies are triggered by administration of KiSS1 gene.…”

“…Reproductive traits are under control of the hypothalamic-pituitary-gonadal (HPG) balance. Among the many genes controlling the reproductive traits, the KiSS1 gene plays a crucial role in the neuroendocrine regulation of GnRH secretion and puberty onset (Takei et al, 2016) in many species, like mice (Lapatto et al, 2007;Ieda et al, 2019), Bos taurus (Divya et al, 2017), Bos indicus (Divya et al, 2017), pig (Tomikawa et al, 2010;Basini et al, 2018) and mouse (Merhi et al, 2016). Tomikawa et al (2010) reported that in porcine, KiSS1 gene was located in chromosome 9 and spans over 6 kb composed of two introns and three exons.…”

Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review

“…In the latter study, KISS1 expression in the pituitary was greater for Jintang goats, and it was suggested that this could be linked with prolificacy. KISS1 SNPs were associated with acrosome integrity and fertility in Holstein Friesian ( Bos taurus ) and Khillari ( B. indicus ) bulls [ 203 ]. Given the peripheral action of KISS1 at reproductive tissues, the discovery and validation of KISS1 and KISS1R SNPs have potential for their inclusion in genomic selection indices for fertility in livestock.…”