Single‐Molecule Technology for Rapid Detection of DNA Hybridization Based on Resonance Light Scattering of Gold Nanoparticles

Wang, Kanglin; Qiu, Xin; Dong, Chaoqing; Ren, Jicun

doi:10.1002/cbic.200700174

Cited by 57 publications

(57 citation statements)

References 22 publications

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“…On comparison, the concentration measurement is difficultly achieved by DLS because it is not sensitive to the concentration change based on its working principle. So far, RLSCS method has been used for characterization on noble metal nanoparticles and their bioconjugates, translational and rotational diffusions of nanoparticles, homogeneous DNA detection, and homogeneous immunoassays [24,25].…”

Section: Introductionmentioning

confidence: 99%

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

et al. 2016

Microchim Acta

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We report on a method for direct determination of the molar concentration of gold nanoparticles (GNP) in solution by using resonance light scattering correlation spectroscopy (RLSCS). RLSCS is based on the correlation analysis of the fluctuations of resonance light scattering due to Brownian motion of single nanoparticles in a highly-focused laser volume. Similar to single molecule fluorescence correlation spectroscopy, the number of particles in the detection volume is reciprocally related to the G(0) value in the RLSCS plot. A model is established for quantification of GNP concentration, and the effect of laser illumination intensity was studied. An excellent linear relationship exists between the concentration of GNP and the reciprocal G(0) value of the correlation plots at low laser illumination intensity. The method was applied to the determination of the molar concentrations of GNP in sizes of 15, 20, 30, and 40 nm to give detection limits of 300, 80, 10, and 40 pM, respectively. The results obtained by RLSCS were in good agreement with that of combined atomic emission spectroscopy and transmission electron microscopy (AES-TEM). A sensitive microscale method was reported for the determination of the activity of the enzyme caspase 3 by RLSCS by using GNP as labeling probes. The assay is based on the measurement of the change of the molar concentration of GNP when peptide-labeled GNP substrates were cleaved by caspase 3. The method is shown to enable the determination of caspase 3 (with a 0.1 nM detection limit) and to monitoring drug-induced cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

et al. 2016

Microchim Acta

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“…Since Pasternack developed a resonance light scattering (RLS) technique with a common spectroflurimeter (12,13), this technique has been widely applied to determine nucleic acids (14)(15)(16) and proteins (17)(18)(19)(20). The RLS technique is based on the following phenomenon: when the wavelength of the incident beam is close to the absorption band of molecular particles which exist as aggregates, the Rayleigh scattering intensity of some wavelengths will be much higher than normal light scattering (21).…”

Section: Introductionmentioning

confidence: 99%

Resonance light‐scattering enhancement effect of the protein–Y³⁺–TTA–SLS system and its analytical application

Guo

et al. 2008

Luminescence

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In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y(3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 x 10(-9)-1.0 x 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6 )g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied.

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“…The detection limit is, in general, at the nmol L À1 level. Recently, resonance Rayleigh scattering [21][22][23][24] and dynamic light scattering [25] techniques were used in homogeneous DNA hybridization with GNPs as probes and the sensitivity was significantly enhanced to about the pmol L À1 level. More recently, our group reported a single-GNP counting technique in solution.…”

Section: Introductionmentioning

confidence: 99%

Retraction: Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single‐Silver‐Nanoparticle Counting

Xu¹,

Dong²,

Xie³

et al. 2010

Chemistry A European J

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Single‐Molecule Technology for Rapid Detection of DNA Hybridization Based on Resonance Light Scattering of Gold Nanoparticles

Cited by 57 publications

References 22 publications

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

Resonance light‐scattering enhancement effect of the protein–Y³⁺–TTA–SLS system and its analytical application

Retraction: Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single‐Silver‐Nanoparticle Counting

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Single‐Molecule Technology for Rapid Detection of DNA Hybridization Based on Resonance Light Scattering of Gold Nanoparticles

Cited by 57 publications

References 22 publications

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

A single particle method for direct determination of molar concentrations of gold nanoparticles, and its application to the determination of the activity of caspase 3 and drug-induced cell apoptosis

Resonance light‐scattering enhancement effect of the protein–Y3+–TTA–SLS system and its analytical application

Retraction: Ultrahighly Sensitive Homogeneous Detection of DNA and MicroRNA by Using Single‐Silver‐Nanoparticle Counting

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Resonance light‐scattering enhancement effect of the protein–Y³⁺–TTA–SLS system and its analytical application