Single‐Molecule Detection of Proteins Using Aptamer‐Functionalized Molecular Electronic Devices

Liu, Song; Zhang, Xinyue; Luo, Weimin; Wang, Zhenxing; Guo, Xuefeng; Steigerwald, Michael L.; Fang, Xiaohong

doi:10.1002/anie.201006469

Cited by 102 publications

(78 citation statements)

References 42 publications

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“…Electronic conductivity of "non-canonical" DNA forms, quadruplexes in particular have also been addressed, but such reports have only begun to appear much more recently [27][28][29][30][31][32][33] , and with little if any direct approach to interfacial ; (c) surface immobilized G-quadruplexes finally offer perspectives for ultra-sensitive detection of metal ions [40][41][42] , complex biomolecules such as peptides [23][24][25][26][27][28][29][30] , and perhaps other analytes.…”

Section: Resultsmentioning

confidence: 99%

Voltammetry and molecular assembly of G-quadruplex DNAzyme on single-crystal Au(111)-electrode surfaces – hemin as an electrochemical intercalator

2016

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Section: Resultsmentioning

confidence: 99%

Voltammetry and molecular assembly of G-quadruplex DNAzyme on single-crystal Au(111)-electrode surfaces – hemin as an electrochemical intercalator

2016

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“…38 The aptamer used here was a 15-nucelotide single-stranded DNA with thymine 7 linkers on both 3′ and 5′ termini. The G4 conformation of this aptamer could be stabilized by K + or Mg 2+ , and had a high binding affinity for thrombin ( Figure 5B).…”

Section: Accounts Of Chemical Researchmentioning

confidence: 99%

Carbon Electrode–Molecule Junctions: A Reliable Platform for Molecular Electronics

et al. 2015

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The development of reliable approaches to integrate individual or a small collection of molecules into electrical nanocircuits, often termed "molecular electronics", is currently a research focus because it can not only overcome the increasing difficulties and fundamental limitations of miniaturization of current silicon-based electronic devices, but can also enable us to probe and understand the intrinsic properties of materials at the atomic- and/or molecular-length scale. This development might also lead to direct observation of novel effects and fundamental discovery of physical phenomena that are not accessible by traditional materials or approaches. Therefore, researchers from a variety of backgrounds have been devoting great effort to this objective, which has started to move beyond simple descriptions of charge transport and branch out in different directions, reflecting the interdisciplinarity. This Account exemplifies our ongoing interest and great effort in developing efficient lithographic methodologies capable of creating molecular electronic devices through the combination of top-down micro/nanofabrication with bottom-up molecular assembly. These devices use nanogapped carbon nanomaterials (such as single-walled carbon nanotubes (SWCNTs) and graphene), with a particular focus on graphene, as point contacts formed by electron beam lithography and precise oxygen plasma etching. Through robust amide linkages, functional molecular bridges terminated with diamine moieties are covalently wired into the carboxylic acid-functionalized nanogaps to form stable carbon electrode-molecule junctions with desired functionalities. At the macroscopic level, to improve the contact interface between electrodes and organic semiconductors and lower Schottky barriers, we used SWCNTs and graphene as efficient electrodes to explore the intrinsic properties of organic thin films, and then build functional high-performance organic nanotransistors with ultrahigh responsivities. At the molecular level, to form robust covalent bonds between electrodes and molecules and improve device stability, we developed a reliable system to immobilize individual molecules within a nanoscale gap of either SWCNTs or graphene through covalent amide bond formation, thus affording two classes of carbon electrode-molecule single-molecule junctions. One unique feature of these devices is the fact that they contain only one or two molecules as conductive elements, thus forming the basis for building new classes of chemo/biosensors with ultrahigh sensitivity. We have used these approaches to reveal the dependence of the charge transport of individual metallo-DNA duplexes on π-stacking integrity, and fabricate molecular devices capable of realizing label-free, real-time electrical detection of biological interactions at the single-event level, or switching their molecular conductance upon exposure to external stimuli, such as ion, pH, and light. These investigations highlight the unique advantages and importance of these universal methodologies to pr...

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“…然而这些研究还是静态的, 并未实现实时地检测生物分子之间的相互作用. 针对这一问题, 为了将快速的实时测量与单分子器件的高灵敏度结合在一起, 我们进一步发展了一套新的测量体系 [22] . DNA 的双螺旋结构具有天然独特的分子识别性质, 能够自组装成特定的二维或三维结构, 因此 DNA 分子可能实现自下而上的分子电子器件和电路的组装 [23,24] .…”

Section: 单壁碳纳米管的结构特征unclassified

“…DNA 的双螺旋结构具有天然独特的分子识别性质, 能够自组装成特定的二维或三维结构, 因此 DNA 分子可能实现自下而上的分子电子器件和电路的组装 [23,24] . 然而, 前期工作表明, DNA 的导电性并不高 [21,22] . ) [46] .…”

Section: 单壁碳纳米管的结构特征unclassified

Functional Single-Walled Carbon Nanotube-based Molecular Devices

Liu¹,

Guo²

2013

Acta Chim. Sinica

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Single‐Molecule Detection of Proteins Using Aptamer‐Functionalized Molecular Electronic Devices

Abstract: Filling in the gap: Label‐free, real‐time electrical detection of proteins is achieved with high selectivity and real single‐molecule sensitivity by using aptamer‐functionalized molecular electronic devices with single‐walled carbon nanotubes as point contacts.

Cited by 102 publications

References 42 publications

Voltammetry and molecular assembly of G-quadruplex DNAzyme on single-crystal Au(111)-electrode surfaces – hemin as an electrochemical intercalator

Voltammetry and molecular assembly of G-quadruplex DNAzyme on single-crystal Au(111)-electrode surfaces – hemin as an electrochemical intercalator

Carbon Electrode–Molecule Junctions: A Reliable Platform for Molecular Electronics

Functional Single-Walled Carbon Nanotube-based Molecular Devices

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