Single-laboratory validation for the determination of caffeic acid and seven caffeoylquinic acids in sweet potato leaves

Abstract

A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of caffeic acid (CA) and seven species of caffeoylquinic acids (CQAs) in lyophilized sweet potato leaves. The procedure for extraction of the analytes from the matrix and the HPLC conditions for the efficient separation of CA and CQAs were optimized. In the proposed method, a relative response factor to one of the CQAs (5-CQA) was used to quantify the others. The method performed well in terms of precision when carried out on five different days and demonstrated Horwitz ratio (HorRat) scores ranging from 0.5 to 1.0 for all analytes, which were well within the limits of performance acceptability. Accuracy testing at three levels showed an overall recovery of 94% when duplicated on five different days. Moreover, a stability study revealed that all analytes in both standard solution and sample extract were stable for 28 days.

Unremitting Problems With Chlorogenic Acid Nomenclature: A Review

“…[6][7][8] Because of these many positive influences on human body, since the middle of the 19 th century, when the first references about CGAs appeared, there have been a high amount of articles dealing with extraction and detection techniques of CGAs or studying their influence on human health. CGAs have been observed and isolated in many plant materials such as coffee 9 , apple 10 , tomato, papaya 11 , sweet potato 12 , prune 13 , pear 14 , cabbage 15 , yacon 16 , burdock 17 , cherry 18 , apricot 19 , orange 20 , etc. Coffee beans are undoubtedly the most common observed matrix because coffee is the main source of CGAs.…”

Effect of repeated harvesting on the content of caffeic acid and seven species of caffeoylquinic acids in sweet potato leaves

“…1} using CA and 5-CQA as relative response factors (RRF), which is calculated by the molar extinction coefficient and the molecular mass of CQAs standards and used to compute absolute but not relative concentration of CQAs in sweet potato leaves. 17) Furthermore, a single-laboratory validation study of the proposed method was successfully performed, and the high accuracy and precision of the method were confirmed. 17) Previous research has shown that cultivars/breeding lines and environmental conditions, such as temperature and intensity of solar radiation, affect CA and CQAs content in sweet potato leaves, 18) but limited *Corresponding author.…”

“…17) Furthermore, a single-laboratory validation study of the proposed method was successfully performed, and the high accuracy and precision of the method were confirmed. 17) Previous research has shown that cultivars/breeding lines and environmental conditions, such as temperature and intensity of solar radiation, affect CA and CQAs content in sweet potato leaves, 18) but limited *Corresponding author. Email: sokuno@affrc.go.jp Abbreviations: AcCN, acetonitrile; CA, caffeic acid; CQA, caffeoylquinic acid; HPLC, high-performance liquid chromatography; RRF, relative response factor.…”

“…As described in a previous study, 17) 5 μL of filtered extract was injected into an HPLC system for analysis. The HPLC system that was used in this study consisted of a model PU-2080i plus pump, a model LG-2080-04 low-pressure gradient unit, a model DG-2080-54 degasser, a model AS-2057 plus auto injector, a model CO-2060 plus column oven, and a model UV-2070 plus detector with a conventional flow cell of 17 μL intrinsic volume (JASCO, Tokyo, Japan).…”

High-performance liquid chromatography as a tool to evaluate the performance of the catalytic wet peroxide oxidation of 4-nitrophenol: pre-validation of analytical methods

“…Nowadays, the so-called Horwitz ratio (HorRat) is widely recognized as a suitable acceptance criterion for the evaluation of the precision of an analytical method, namely by several research groups (Brown and Yu 2013, Indyk et al 2014, Sasaki et al 2014 and organizations worldwide (Horwitz andAlbert 2006, Latimer 2012). Specifically, the HorRat is a normalized performance parameter indicating the acceptability of the precision of an analytical method, which is obtained by the ratio between the observed RSD and the predicted relative standard deviation (PRSD) calculated from the Horwitz equation, as described in Eq.…”