Single Fluorescent Probe Separately and Continuously Visualize H<sub>2</sub>S and HClO in Lysosomes with Different Fluorescence Signals

et al. 2021

Chem. Sci.

Section: Introductionmentioning

confidence: 99%

Time-resolved analysis of photoluminescence at a single wavelength for ratiometric and multiplex biosensing and bioimaging

Dai

et al. 2021

Chem. Sci.

“…Hypochlorous acid, a kind of ROS, usually exists in the form—ClO − in physiological pH ( Li et al, 2012 ; Ren et al, 2019 ; Zhang et al, 2020a ). Due to its oxidative properties, ClO − is used in the immune defense system and plays an important role in a variety of physiological and pathological processes.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial-Targeted Ratiometric Fluorescent Probe to Monitor ClO− Induced by Ferroptosis in Living Cells

Feng

et al. 2022

Front. Chem.

Ferroptosis is a type of iron-dependent programmed cell death. Once such kind of death occurs, an individual cell would undergo a series of changes related to reactive oxygen species (ROS) in mitochondria. A mitochondrial-targeted ratiometric fluorescent probe (MBI-OMe) was developed to specifically detect ferroptosis-induced ClO−, whose recognition group is p-methoxyphenol, and the mitochondrial-targeted group is benzimidazole. The fluorescence of MBI-OMe was first quenched by 30 μM of Fe3+, and then MBI-OMe appeared as a ratiometric signal at 477 nm and 392 nm in response to ferroptosis-induced ClO− in living cells. MBI-OMe was successfully used to evaluate changes in ClO− induced by ferroptosis.

“…Specifically, a red‐emitting AIE molecule, DTPA (4,4′‐([1,2,5]thiadiazolo[3,4‐c]pyridine‐4,7‐diyl)bis( N , N ‐diphenylaniline)), is utilized to obtain a high signal‐to‐noise (S/N) ratio and high photobleaching resistance. [ 36 ] Triphenylphosphonium (TPP) [ 33 ] and morpholinyl (MP) [ 37 ] are employed as targeting groups, endowing FNPs with precise mitochondria and lysosome targeting abilities, respectively. CPCDs are conjugated on the surface to expedite cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

Tailorable Membrane‐Penetrating Nanoplatform for Highly Efficient Organelle‐Specific Localization

Zhang

Feng

et al. 2021

Small

Given the breadth of currently arising opportunities and concerns associated with nanoparticles for biomedical imaging, various types of nanoparticles have been widely exploited, especially for cellular/subcellular level probing. However, most currently reported nanoparticles either have inefficient delivery into cells or lack specificity for intracellular destinations. The absence of well‐defined nanoplatforms remains a critical challenge hindering practical nano‐based bio‐imaging. Herein, the authors elaborate on a tailorable membrane‐penetrating nanoplatform as a carrier with encapsulated actives and decorated surfaces to tackle the above‐mentioned issues. The tunable contents in such a versatile nanoplatform offer huge flexibility to reach the expected properties and functions. Aggregation‐induced emission luminogen (AIEgen) is applied to achieve sought‐after photophysical properties, specific targeting moieties are installed to give high affinity towards different desired organelles, and critical grafting of cell‐penetrating cyclic disulfides (CPCDs) to promote cellular uptake efficiency without sacrificing the specificity. Hereafter, to validate its practicability, the tailored nano products are successfully applied to track the dynamic correlation between mitochondria and lysosomes during autophagy. The authors believe that the strategy and described materials can facilitate the development of functional nanomaterials for various life science applications.