Single-Component Equilibrium Isotherms

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

“…The a-la concentration was increased (0.1-4.0 mg mL À1 ) to obtain the breakthrough curves. The adsorbed amount q i+1 is given by [48]:…”

“…Adsorption isotherms were determined by frontal analysis method, being the measurements carried out using the step-wise technique [48][49][50][51] at pH 2.5, temperatures of 30 C and 40 C and different NaCl concentrations (0-180 mmol L À1 ). The experiments were conducted in duplicate, using a HPLC system (Shimadzu, Japan) at 280 nm and flow rate of 1 mL min À1 .…”

Model-based description of peptide retention on doped reversed-phase media

“…For this, the retention factors on the RP material are first measured from experiments in diluted and isocratic conditions as described in [12,18]. These are then fitted with the linear solvent strength equation [12][13][14] with respect to the modifier concentration, and according to the work of Gétaz et al [16] with respect to the ionic strength.…”

“…The Henry coefficient describes the thermodynamic partition of an analyte between stationary and mobile phases [18]. As the hydrophobic part of the DRP surface is identical to that of the RP material, and the only difference between the two is the loss of adsorption area on the DRP material, the Henry coefficient of the DRP material is simply given by the Henry coefficient on the RP material, reduced by the lost area:…”