Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Machado, Ana Paula da Fonseca; Minim, Luís Antônio; Fontan, Rafael da Costa Ilhéu; Minim, Valéria Paula Rodrigues; Gonçalves, Gabriel Ramos Ferreira; Mól, Paula Chequer Gouveia

doi:10.1016/j.fluid.2015.05.021

Cited by 22 publications

(25 citation statements)

References 65 publications

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“…6. The D ax values varied from 10 -8 to 10 -5 m 2 /s and are similar to the range of values obtained by Yao et al [4,24] , Carvalho et al [34] and Machado et al [5] for supermacroporous cryogels, indicating that axial dispersion is weak in the affinity column.…”

Section: Affinity Cryogel Characterizationsupporting

confidence: 87%

“…[1][2][3][4][5][6] Among the materials available for use in purification processes is the supermacroporous monolithic cryogel. By presenting a highly porous structure, with large pores, this cryogel presents high permeability and low flow resistance.…”

Section: Introductionmentioning

confidence: 99%

“…This matrix is an alternative to the direct purification of biocompounds from viscous means and non-clarified media, reducing the number of steps in the purification process and helping to maintain the integrity of the compounds of interest. [2,4,5,7] Despite the advantages presented by the cryogels, the large pores make the adsorption surface area smaller compared to a packaged fixed bed, which tends to lower its efficiency.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…[1,2,5,7,8] Modifications, whether chemical or physical, may be made aiming to increase the selectivity efficiency of the separation processes. [1,5,7,8] Some modifications already studied involve immobilizing derivatives of acrylic acid, [9] metals, [2,8,10] sulfo group, [5,7] aminoacids and proteins, [6] among others in the polymeric matrix, increasing the retention capacity of the compounds. [1,2,5,7,8,11,12] A c c e p t e d M a n u s c r i p t 3 The lysozymes (EC 3.2.1.7) had bactericidal and bacteriostatic properties and are one of the few natural antimicrobial agents approved by regulatory agencies for use in foods.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of an affinity cryogel column for lysozyme purification

Veríssimo

Paganoto

Mól

et al. 2017

Separation Science and Technology

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Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryocopolymerization at −12 °C. A central composite rotational design 2 2 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13 %. Results showed that the affinity cryogel was a potential separation medium for LYZ purification.

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Section: Affinity Cryogel Characterizationsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation of an affinity cryogel column for lysozyme purification

Veríssimo

Paganoto

Mól

et al. 2017

Separation Science and Technology

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Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Silva

Nascimento

et al. 2019

J of Applied Polymer Sci

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The use of macroporous monolithic matrices in the purification of biocompounds is constantly growing and developing. In this work, the objective was to optimize the quantity of N-acetyl-D-glucosamine (D-GlcNAc) immobilized on the surface of macroporous polymeric cryogels for capture of lectins from less clarified solutions. Surface response methodology was applied and it was observed that the immobilization temperature of the glutaraldehyde (GLU) and the D-GlcNAc concentration influenced the amount of sugar immobilized. The matrices produced with 1.1% of allyl glycidyl ether were functionalized by GLU. Optimal maximum condition was obtained with mean value of 160.39 AE 26.38 mg of D-GlcNAc immobilized per gram of dry cryogel. Characterization analyses of the matrices showed that the activation process was effective, maintaining the macroporous structure and physical characteristics. The adsorbents produced were tested for capture of lectins from a crude protein solution of barley. At tested conditions, adsorbent capture around 11% of protein in solution but reduce the hemaglutinating capacity in 40%, demonstrating its selectivity. The cryogels functionalized with D-GlcNAc present potential for use in capture compounds by affinity with carbohydrates, such as lectins.

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Hydrodynamics and dynamic capacity of cryogels produced with different monomer compositions

Chaves

Mól

Minim

et al. 2019

J of Applied Polymer Sci

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Cryogels have received great attention in the area of bioseparation due to their characteristic of very large and interconnected pores. These materials allow rapid isolation of various biomolecules and permit that chromatographic processes are carried out at high flow rates. Nevertheless, studies regarding the effect of monomer concentration on the hydrodynamics and dynamic capacity of cryogels are scarce. In this study, supermacroporous polyacrylamide cryogels with different monomer and crosslinker concentrations were produced by cryopolymerization and activated with tris(hydroxymethyl)aminomethane. Porosity and permeability were determined and it was observed that these parameters changed with different monomer concentrations, indicating changes in the structure of the cryogels. Residence time distribution experiments were conducted at different flow rates and different mobile phase viscosities. As a function of composition of the cryogels, the axial dispersion increased and the flow pattern deviated considerably from the ideal plug flow model. For cryogels with lower crosslinker content, the effect of the mobile phase viscosity was more evident. The monomer composition changed the dispersion number and height equivalent to one theoretical plate, resulting in an impact on the adsorption capacity of the cryogel. Consequently, proper monomer and crosslinker concentrations may produce cryogels with suitable performance. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48507.

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Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Cited by 22 publications

References 65 publications

Preparation of an affinity cryogel column for lysozyme purification

Preparation of an affinity cryogel column for lysozyme purification

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Hydrodynamics and dynamic capacity of cryogels produced with different monomer compositions

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