Single-Cell Transcriptomics Reveals That Metabolites Produced by Paenibacillus bovis sp. nov. BD3526 Ameliorate Type 2 Diabetes in GK Rats by Downregulating the Inflammatory Response

Qiao, Zhenyi; Wang, Xiaohua; Zhang, Huanchang; Han, Jin; Feng, Huafeng; Wu, Zhengjun

doi:10.3389/fmicb.2020.568805

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…Paenibacillus are endospore-forming, facultative anaerobes well known as plant-growth promoters [ 57 ]. Metabolites produced by Paenibacillus have been reported to downregulate inflammatory response and increase regulatory T cell numbers in the intestine of Goto–Kakizaki rats, a spontaneous animal model of type 2 diabetes [ 58 ]. In accordance, we found that Paenibacillus was positively associated with the foxp3 expression, suggesting a putative link between the enrichment of Paenibacillus and increased expression of foxp3 in salmon fed the insect meal diet.…”

Section: Discussionmentioning

confidence: 99%

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Gajardo

Jaramillo-Torres

et al. 2022

anim microbiome

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Background Being part of fish's natural diets, insects have become a practical alternative feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly (Hermetia illucens) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. Results The insect meal diet markedly modulated the salmon intestinal microbiota. Salmon fed the insect meal diet showed similar or lower alpha-diversity indices in the digesta but higher alpha-diversity indices in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were also present in the feeds. Conclusions We conclude that salmon fed the insect meal diets show consistent changes in the intestinal microbiota. The next challenge is to evaluate the extent to which these alterations are attributable to feed microbiota and dietary nutrients, and what these changes mean for fish physiology and health.

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Section: Discussionmentioning

confidence: 99%

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Gajardo

Jaramillo-Torres

et al. 2022

anim microbiome

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“…Dendritic cells, functioning as autocrine or paracrine modulators, synthesize and release classical neurotransmitters crucial for maintaining intestinal immune balance. Their abundance is markedly elevated in the gut of patients with UC and T2DM ( 30 , 31 ). In ulcerative colitis, the usual equilibrium of intestinal B-cell reactions is disturbed, resulting in a notable decrease in regulatory B cells ( 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the shared mechanisms of Gegen Qinlian Decoction in treating type 2 diabetes and ulcerative colitis via bioinformatics and machine learning

Hu,

Xiong,

et al. 2024

Front. Med.

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BackgroundAlthough previous clinical studies and animal experiments have demonstrated the efficacy of Gegen Qinlian Decoction (GQD) in treating Type 2 Diabetes Mellitus (T2DM) and Ulcerative Colitis (UC), the underlying mechanisms of its therapeutic effects remain elusive.PurposeThis study aims to investigate the shared pathogenic mechanisms between T2DM and UC and elucidate the mechanisms through which GQD modulates these diseases using bioinformatics approaches.MethodsData for this study were sourced from the Gene Expression Omnibus (GEO) database. Targets of GQD were identified using PharmMapper and SwissTargetPrediction, while targets associated with T2DM and UC were compiled from the DrugBank, GeneCards, Therapeutic Target Database (TTD), DisGeNET databases, and differentially expressed genes (DEGs). Our analysis encompassed six approaches: weighted gene co-expression network analysis (WGCNA), immune infiltration analysis, single-cell sequencing analysis, machine learning, DEG analysis, and network pharmacology.ResultsThrough GO and KEGG analysis of weighted gene co-expression network analysis (WGCNA) modular genes and DEGs intersection, we found that the co-morbidity between T2DM and UC is primarily associated with immune-inflammatory pathways, including IL-17, TNF, chemokine, and toll-like receptor signaling pathways. Immune infiltration analysis supported these findings. Three distinct machine learning studies identified IGFBP3 as a biomarker for GQD in treating T2DM, while BACE2, EPHB4, and EPHA2 emerged as biomarkers for GQD in UC treatment. Network pharmacology revealed that GQD treatment for T2DM and UC mainly targets immune-inflammatory pathways like Toll-like receptor, IL-17, TNF, MAPK, and PI3K-Akt signaling pathways.ConclusionThis study provides insights into the shared pathogenesis of T2DM and UC and clarifies the regulatory mechanisms of GQD on these conditions. It also proposes novel targets and therapeutic strategies for individuals suffering from T2DM and UC.

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Tibetan Plateau yak milk: A comprehensive review of nutritional values, health benefits, and processing technology

Li,

Liu,

Han

et al. 2023

Food Chemistry: X

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Single-Cell Transcriptomics Reveals That Metabolites Produced by Paenibacillus bovis sp. nov. BD3526 Ameliorate Type 2 Diabetes in GK Rats by Downregulating the Inflammatory Response

Cited by 4 publications

References 41 publications

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Deciphering the shared mechanisms of Gegen Qinlian Decoction in treating type 2 diabetes and ulcerative colitis via bioinformatics and machine learning

Tibetan Plateau yak milk: A comprehensive review of nutritional values, health benefits, and processing technology

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