Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats

Yin, Xiufeng; Cheng, Guoxiang; Guo, Haiyan; Wang, Q.; Li, Y.J.; Zhang, Hao

doi:10.4238/gmr16019564

Cited by 8 publications

(7 citation statements)

References 33 publications

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“…Single-cell RNA sequencing (scRNA-seq) provides an alternative method for studying the cellular heterogeneity of human preimplantation embryos and embryonic stem cells [8,9] , mouse oocytes [10] , porcine oocyte maturation [11] , and Haimen white goat's oocyte maturation by generating a readout of the abundance of a transcript within a cell [12] . Indeed, scRNA-seq applied to mammalian gametes has generated new insights into the composition of transcripts and disease-related functional abnormalities.…”

Section: Introductionmentioning

confidence: 99%

Identification and functional analysis of transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing from the oocyte to the preimplantation stage of sheep by single-cell RNA sequencing

Zhang¹,

Shi²,

Zhu³

et al. 2023

Preprint

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Numerous dynamic and complicated processes characterize development from the oocyte to the embryo. However, given the importance of functional transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing during embryonic development, the effect that these features have on the blastomeres of 2-, 4-, 8-, 16-cell, and morula stages of development have not been studied. Here, we conducted a scRNA-seq survey of cells from sheep from the oocyte to the blastocyst developmental stages. We then carried out experiments to identify and functionally analyze the transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms (SNPs), and alternative splicing (AS). We founded that between the oocyte and zygote groups significantly down-regulated genes and the second-largest change in gene expression occurred between the 8- and 16-cell stages. We used various methods to construct a profile to characterize cellular and molecular features and systematically analyze the related GO and KEGG profile of cells of all stages from the oocyte to the blastocyst. This large-scale, single-cell atlas provides key cellular information and will likely assist clinical studies in improving preimplantation genetic diagnosis.

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Section: Introductionmentioning

confidence: 99%

Identification and functional analysis of transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing from the oocyte to the preimplantation stage of sheep by single-cell RNA sequencing

Zhang¹,

Shi²,

Zhu³

et al. 2023

Preprint

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“…The in vitro maturation of oocytes was considered to be an important technical link of embryo engineering technology, which aims to further explore the potential of animal reproduction and provide effective solutions for large numbers of breeding high‐quality livestock, such as cattle (Zoheir et al., 2017) and goats (Ren et al., 2018; Yin et al., 2017). In vitro production (IVP) can improve the original genetic characteristics and reproductive capacity of oocytes through artificial factors, which has great potential for the research of improving livestock, such as goats (Fernandes et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

Alpha‐lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro

Wang

Zhang

et al. 2021

Reprod Domestic Animals

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Oxidative stress inevitably occurs during oocyte maturation in vitro. α‐lipoic acid (α‐LA) has a strong antioxidant capacity, but the effect of α‐LA on parthenogenetic activation of oocytes was rarely reported. This study aims to investigate the effect of supplementing α‐LA to in vitro maturation medium on the subsequent developmental ability of goat parthenogenetic embryos during oocytes maturation. In the study, the goat cumulus‐oocyte complex was divided into the experimental (with 25 μmol/L α‐LA) and the control (without α‐LA) groups. Oxidase expression was measured using RT‐qPCR. After 18–22 hr of maturation, the oocytes were then parthenogenetic activated. The total antioxidant capacity of embryos was measured after 0, 24, 48, 72 and 96 hr of culture. Rates of oocyte maturation and the rates of development for parthenogenetic embryos in the α‐LA group were significantly improved by 7.88% (p < .05) and 5.41% (p < .05) compared with those in the control group, respectively. After 24 hr, the difference in total antioxidant capacity was extremely significant in both groups. An evident decrease in the control group and a minor decrease in the α‐LA group were observed (p < .01). The ratio of inner cell mass cells to the total cell number of blastocysts in the α‐LA group increased compared with that in the control group (p < .05) on day 8. α‐LA significantly promoted the expression of SOD and GPX4 of parthenogenetic blastocysts and maturated oocytes. α‐LA (25 μmol/L) improved the maturation rate and the developmental competence of the parthenogenetic activation of oocytes, which might be mediated by maintaining the total antioxidant ability of oocytes during the culture period.

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“…Similarly, single-cell transcriptomic analysis reveals heterogeneous differentiation and spatial characteristics of mouse epidermis and hair follicles ( Joost et al, 2016 ). Furthermore, to determine the differences in oocyte maturation at different stages, transcriptome sequencing of single cells was compared with oocyte maturation gene expression in children and female goats to screen and verify genes related to oocyte maturation ( Yin et al, 2017 ). However, research on regulating the fineness of cashmere at the cellular level is still scarce.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

Wang

Hui

et al. 2021

Front. Genet.

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Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells.

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Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats

Cited by 8 publications

References 33 publications

Identification and functional analysis of transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing from the oocyte to the preimplantation stage of sheep by single-cell RNA sequencing

Identification and functional analysis of transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing from the oocyte to the preimplantation stage of sheep by single-cell RNA sequencing

Alpha‐lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro

Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

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