Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise

Yu, Yanli; Zhang, Xiaolu; Chen, Yanjing; Li, Yanze; Bian, Suying; Yang, Yizhuo; Song, Hanan; Zhang, Cheng; Dong, Zhenhe; Li, Guanghui; Xu, Luyou; Jia, Zhang; Liu, Yu; Xiao, He; Yang, Ming–Hsuan; Guo, Jianjun; Zhou, Zucheng; Zhang, Yan

doi:10.1016/j.isci.2023.106532

Cited by 2 publications

(2 citation statements)

References 43 publications

(51 reference statements)

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“…Compared to those on D1, the proportions of cytotoxic CD8 + T, γδT and CD16 + NK cells on D16 were significantly lower, accompanied by the downregulated expression of genes associated with immune effects, including GZMA, GZMK, GZMH, NKG7, KLRD1, CD160 and CX3CR1. These findings are consistent with the observed decrease in the percentage of cytotoxic T cells following a marathon [20]. The impairment of T cell-mediated function at extreme altitudes might disrupt immune homeostasis and increase the risk of infectious disease.…”

Section: Discussionsupporting

confidence: 89%

“…The emergence of single-cell RNA sequencing (scRNA-seq) has facilitated an in-depth dissection of immune response changes driven by exercise and has been applied to exercise immunology [20]. To elucidate the immune response and metabolic alterations during high-altitude mountaineering, we performed multi-omics profiling, including targeted metabolomics and lipidomics of plasma, as well as single-cell transcriptome analysis of circulating immune cells, on each sample from eleven mountain climbers (Figure 1A).…”

Section: Introductionmentioning

confidence: 99%

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Multi-omics Reveals Immune Response and Metabolic Profiles during High-Altitude Mountaineering

Yin,

Lv,

Yang

et al. 2024

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SUMMARYThe physiological perturbations induced by high-altitude exposure in mountain climbers, manifesting as metabolic and immunologic deviations, have been previously reported but are not fully understood. In this study, we obtained longitudinal multi-omic profiles of blood samples for healthy mountain climbers during two mountaineering stages (acclimatization and extreme altitude mountaineering). Our integrative assay included metabolomics and lipidomics profiling of plasma coupled with single-cell transcriptomic analysis of 375,722 immune cells. Longitudinal analysis revealed dynamic immune response profiles, during the acclimatization period, characterized by the downregulation of inflammatory responses in monocytes and classical dendritic cells (cDCs) and an increase in the proportion of cytotoxic CD8+T cells with enhanced immune effector processes. In contrast, during extreme altitude mountaineering, the activation of inflammatory responses and impairment of T cell effector function were observed, concomitant with an increased cellular response to hypoxia and oxidative stress pathways. Furthermore, we found upregulated glycolysis and antioxidant gene expression during extreme altitude mountaineering, which was primarily orchestrated byHIF1AandNFE2L2, while decreased expression of these genes was observed in dysregulated plasmacytoid dendritic cells (pDCs). Finally, high-resolution plasma metabolic analysis revealed significant alterations in the metabolism of climbers, involving enhanced glutamine and fatty acid metabolism.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%