Single-cell RT-PCR, in situ hybridization histochemical, and immunohistochemical studies of substance P and enkephalin co-occurrence in striatal projection neurons in rats

Wang, H. B.; Laverghetta, Antonio; Foehring, Robert C.; Deng, Yunping; Sun, Zhiqiang; Yamamoto, Kei; Lei, Wanlong; Jiao, Yun; Reiner, Anton

doi:10.1016/j.jchemneu.2006.01.003

Cited by 39 publications

(44 citation statements)

References 62 publications

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“…3C). This observation is consistent with the findings that not all striatal projection neurons contain immunohistochemically detectible CALB in rodents (Wang et al, 2006), nor do large striatal interneurons (Gerfen and Wilson, 1996). In contrast to the ubiquity of GluR2 in calbindinergic striatal neurons, not all striatal projection neurons of the matrix compartment, as identified by their uniform perikaryal Alexa 594 (red) immunolabeling for CALB (Fig 3A), also showed an Alexa 488 (green) cytoplasmic ring of GluR1 immunofluorescence (Fig.…”

Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typessupporting

confidence: 91%

“…Note that since striato-GPi and striatonigral neurons send a minor collateral into GPe (Kawaguchi et al, 1990;Wu et al, 2000), the RDA3k injection into GPe may have also labeled some striato-GPi or striatonigral neurons. A prior single-cell RT-PCR study of ours (Wang et al, 2006), however, indicates that no more than 25% of striatal neurons retrogradely labeled from GPe are striato-GPi or striatonigral neurons. Similarly, retrograde tracer injections into GPi might yield labeling of striatonigral neurons via their fibers of passage through the GPi, and retrograde tracer injection into nigra might yield labeling of striato-GPi neurons via their collaterals in the nigra (Kawaguchi et al, 1990;Wu et al, 2000).…”

Section: Rda3k Retrograde Labeling Of Striatal Projection Neuronscontrasting

confidence: 62%

“…3C). Again, this reflects the findings that not all striatal projection neurons contain detectible CALB in rodents (Wang et al, 2006), and large striatal interneurons lack CALB as well (Gerfen and Wilson, 1996). In particular, the large, intensely GluR1+ perikarya did not label for CALB.…”

Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typessupporting

confidence: 56%

“…For example, GluR1 was most prevalent among striatal neurons retrogradely labeled from GPe (71.6%), next most prevalent among those labeled from the nigra (62.2%), and least prevalent among those labeled from the GPi (53.4%). Note that since about 25% of striatal neurons retrogradely labeled from GPe are striato-GPi/nigral neurons labeled via their slight intra-GPe collateral (Wang et al, 2006), and since we found GluR1 frequency to be only 50-60% among striato-GPi/nigral neurons, it seems likely that GluR1 frequency among true striato-GPe neurons is somewhat higher than the 71.6% shown in the table for neurons labeled from GPe. Similarly, striatal neurons labeled from GPi include some striatonigral neurons labeled via their axon passing through GPi, and striatal neurons labeled from the nigra include some striato-GPi neurons labeled via their collateral to the nigra.…”

Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typesmentioning

confidence: 54%

“…Similarly, retrograde tracer injections into GPi might yield labeling of striatonigral neurons via their fibers of passage through the GPi, and retrograde tracer injection into nigra might yield labeling of striato-GPi neurons via their collaterals in the nigra (Kawaguchi et al, 1990;Wu et al, 2000). Prior studies suggest, however, that any such unintended labeling is not so extensive (about 20% labeled from GPi may be striatonigral and 20% from nigra may be striato-GPi) as to obscure trends for the population intended for retrograde labeling (FigueredoCardenas et al, 1994;Wang et al, 2006).…”

Section: Rda3k Retrograde Labeling Of Striatal Projection Neuronsmentioning

confidence: 99%

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Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats

Deng

Xie

Wang

et al. 2007

Journal of Chemical Neuroanatomy

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Differences among the various striatal projection neuron and interneuron types in cortical input, function, and vulnerability to degenerative insults may be related to differences among them in AMPA-type glutamate receptor abundance and subunit configuration. We therefore used immunolabeling to assess the frequency and abundance of GluR1 and GluR2, the most common AMPA subunits in striatum, in the main striatal neuron types. All neurons projecting to the external pallidum (GPe), internal pallidum (GPi) or substantia nigra, as identified by retrograde labeling, possessed perikaryal GluR2, while GluR1 was more common in striato-GPe than striato-GPi perikarya. The frequency and intensity of immunostaining indicated the rank order of their perikaryal GluR1:GluR2 ratio to be striato-GPe > striatonigral > striato-GPi. Ultrastructural studies suggested a differential localization of GluR1 and GluR2 to striatal projection neuron dendritic spines as well, with GluR1 seemingly more common in striato-GPe spines and GluR2 more common in striato-GPi and/or striatonigral spines. Comparisons among projection neurons and interneurons revealed GluR1 to be most common and abundant in parvalbuminergic interneurons, and GluR2 most common and abundant in projection neurons, with the rank order for the GluR1:GluR2 ratio being parvalbuminergic interneurons > calretinergic interneurons > cholinergic interneurons > projection neurons > somatostatinergic interneurons. Striosomal projection neurons had a higher GluR1:GluR2 ratio than did matrix projection neurons. The abundance of both GluR1 and GluR2 in striatal parvalbuminergic interneurons and projection neurons is consistent with their prominent cortical input and susceptibility to excitotoxic insult, while differences in GluR1: GluR2 ratio among projection neurons are likely to yield differences in Ca2+ permeability, desensitization, and single channel current, which may contribute to differences among them in plasticity, synaptic integration, and excitotoxic vulnerability. The apparent association of the GluR1 subunit with synaptic plasticity, in particular, suggests striato-GPe neuron spines as a particular site of corticostriatal synaptic plasticity, presumably associated with motor learning.

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Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typessupporting

confidence: 91%

Section: Rda3k Retrograde Labeling Of Striatal Projection Neuronscontrasting

confidence: 62%

Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typessupporting

confidence: 56%

Section: Frequency Of Glur1 and Glur2 In Striatal Neuron Typesmentioning

confidence: 54%

Section: Rda3k Retrograde Labeling Of Striatal Projection Neuronsmentioning

confidence: 99%

See 3 more Smart Citations

Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats

Deng

Xie

Wang

et al. 2007

Journal of Chemical Neuroanatomy

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Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats

Lei

Deng

Liu

et al. 2013

J of Comparative Neurology

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We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axo-spinous terminals had a mean diameter of 0.624 lm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 µm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thala-mostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons.

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Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats

Lei¹,

Deng²,

Liu³

et al. 2013

J of Comparative Neurology

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We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axo-spinous terminals had a mean diameter of 0.624 lm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 µm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thala-mostriatal terminals contact both

show abstract

Single-cell RT-PCR, in situ hybridization histochemical, and immunohistochemical studies of substance P and enkephalin co-occurrence in striatal projection neurons in rats

Cited by 39 publications

References 62 publications

Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats

Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats

Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats

Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats

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