Single-cell RNA landscape of the special fiber initiation process in Bombax ceiba

Advances in Single-Cell Transcriptome Sequencing and Spatial Transcriptome Sequencing in Plants

“…scRNA-seq of the Bombax ceiba ovary resulted in the identification of a total of 15,567 high-quality cells from the inner wall of the cotton ovary, the identification of 347 potential marker genes for the fiber initiation cell type, and the identification of two major cell types, fiber initiation cells and epidermal cells, by RNA in situ hybridization. As well as the major marker genes in the two cell types, PDF1 in initiating fiber cells and HIC in epidermal differentiation, the study of B. ceiba fiber initiation contributes to the improvement of cotton fiber yield [ 47 ]. In 2023, Lee et al collected more than 800,000 Arabidopsis nuclei located at different developmental stages and in different organ tissues for snRNA-seq and constructed a single-nucleus transcriptional atlas of Arabidopsis between the seed and the production of new seed, which provides a resource for the study of the characterization of the cell types of the plant throughout its development [ 52 ].…”

“…ScRNA-seq developmental trajectories and regulatory networks [40] Manihot esculenta leaf developmental trajectories and photosynthesis [41] Camellia sinensis leaf gene expression network [42] Hevea brasiliensis leaf cell-specific gene expression patterns [43] Taxus wallichiana var. chinensis leaf gene regulatory network [44] Fragaria × ananassa leaf aingle-cell mapping after infection [45] Nicotiana tabacum corolla single-cell metabolic heterogeneity [46] Bombax ceiba capsule cell developmental trajectories and regulatory networks [47] Camellia oleifera ovule gene regulatory network [48] Gossypium ovule gene regulatory network [49,50] Solanum lycopersicum stem SnRNA-seq spatial and temporal development trajectory [51] Arabidopsis thaliana seed construction of cell map [52] Mesembryanthemum crystallinum leaf gene expression pattern [53] Arabidopsis thaliana leaf Spatial transcriptome sequencing spatial expression patterns of genes [54] inflorescence spatial development characteristics [55,56] Populus tremula leafbud spatial development characteristics [55,56] Picea abies female cone spatial development characteristics [55][56][57] Solanum lycopersicum fruit spatial development trajectory [58] callus gene regulatory network [59] Brassica rapa var. glabra leaf gene regulatory network/heat stress [60]/ [61] Portulaca oleracea leaf spatial gene expression patterns [62] Phalaenopsis aphrodite flower spatial and temporal trajectory of development [63] Populus stem gene regulatory network [64] Arabidopsis thaliana leaf ScRNA-seq + single -cell transcriptome spatial development trajectory [65] Populus stem gene regulatory network [66] 2.2.1.…”

“…fruit/seed chalazal cyst AT2G44240, AT4G13380 [26] chalazal nodule AT5G10440, AT1G44090 [26] initiated fiber cell cluster HD1, BZR1, MYB2, TRY, HOX1 [47] epidermal cell HIC, CoAKR, CoCYP81E8, CoUGT73C5, CoUGT91A1 [47,48] synergid cell CoECA1 [48] procambium cell CoDOF4.6 [48] fibre cell MYB25-like, MYB25, MML9, HOX3, XTH [49,50] ovule ToFZY2, ToFZY3, Solyc03g112460, PIN5, ARF7, ARF9, IAA14, IAA35 [58] ovary wall ToFZY1, ToFZY5 [58] ovary wall/pericarp ToFZY5, PIN6, PIN7, PIN1, LAX3, ARF9, IAA17…”

Unraveling Developmental Dynamics and Triterpene Saponin Biosynthesis in Panax notoginseng Stem Apices by Single-Cell Transcriptomics

“…Notably, the roots of Lotus japonicus and the stems of Populus L. have also garnered research interest [35][36][37]. Among cash crops, peanut leaves, tea leaves, tobacco corollas, strawberry leaves, cotton ovules and leaves, and Bombax ceiba have attracted extensive interest [38][39][40][41][42][43][44]. Furthermore, the medicinal plant has placed particular emphasis on the leaves of Nepeta tenuifolia, the foliage of Catharanthus roseus, and the stems and leaves of Taxus mairei [45][46][47][48].…”