Single-cell optoporation and transfection using femtosecond laser and optical tweezers

Waleed, Muhammad; Hwang, Sun-Uk; Kim, Jungdae; Shabbir, Irfan; Shin, Sang-Mo; Lee, Yong-Gu

doi:10.1364/boe.4.001533

Cited by 69 publications

(57 citation statements)

References 46 publications

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“…The technique has several unique advantages including non-contact, cell-friendly and ultra-high force resolution for studying single-cell mechanics [83]. More importantly, OT are able to manipulate cells within liquid medium which makes it the most suitable technique to study cellular mechanical behaviours in cavitation flow [84]. Recently, OT have emerged as a novel tool for manipulating single biological cells and performing sophisticated biomechanical characterizations such as studying the mechanics of a single liposome [85] as well as characterizing the mechanical properties of single biological cells [86].…”

Section: Optical Tweezersmentioning

confidence: 99%

Cell mechanics in biomedical cavitation

2015

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Studies on the deformation behaviours of cellular entities, such as coated microbubbles and liposomes subject to a cavitation flow, become increasingly important for the advancement of ultrasonic imaging and drug delivery. Numerical simulations for bubble dynamics of ultrasound contrast agents based on the boundary integral method are presented in this work. The effects of the encapsulating shell are estimated by adapting Hoff's model used for thin-shell contrast agents. The viscosity effects are estimated by including the normal viscous stress in the boundary condition. In parallel, mechanical models of cell membranes and liposomes as well as state-of-the-art techniques for quantitative measurement of viscoelasticity for a single cell or coated microbubbles are reviewed. The future developments regarding modelling and measurement of the material properties of the cellular entities for cutting-edge biomedical applications are also discussed.

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Section: Optical Tweezersmentioning

confidence: 99%

Cell mechanics in biomedical cavitation

2015

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“…The particle could get into the liposome by both optical and chemical method. The particle can be brought into the cell by cutting a hole into the cell membrane with a femetosecond laser and trapping the particle through the hole with an optical trap [12,13]. We test different methods of making liposome to find as discussed below Figure 2. …”

Section: Liposome Productionmentioning

confidence: 99%

Viscoelasticity measurements inside liposomes

et al. 2014

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Microrheology, the study of the behavior of fluids on the microscopic scale, has been and continues to be one of the most important subjects that can be applied to characterize the behavior of biological fluids. It is extremely difficult to make rapid measurement of the viscoelastic properties of the interior of living cells. Liposomes are widely used as model system for studying different aspects of cell biology. We propose to develop a microrheometer, based on real-time control of optical tweezers, in order to investigate the viscoelastic properties of the fluid inside liposomes. This will give greater understanding of the viscoelastic properties of the fluids inside cells.In our experiment, the liposomes are prepared by different methods to find out both a better way to make GUVs and achieve efficient encapsulation of particle. By rotating the vaterite inside a liposome via spin angular momentum, the optical torque can be measured by measuring the change of polarization of the transmitted light, which allows the direct measurement of viscous drag torque since the optical torque is balanced by the viscous drag. We present an initial feasibility demonstration of trapping and manipulation of a microscopic vaterite inside the liposome. The applied method is simple and can be extended to sensing within the living cells

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“…In its standard form, pores are created in the cell membrane by high-intensity femtosecond (fs) laser pulses that are focused precisely with respect to the cell membrane ( Figure 1) [61][62][63][64][65][66]. In the context of this review, the term 'pore' will be used to denote both water-filled holes in the membrane as well as local zones with increased membrane permeability, for instance, by localized reorganization of lipids.…”

Section: Introductionmentioning

confidence: 99%

Laser-assisted photoporation: fundamentals, technological advances and applications

Xiong

Samal

Demeester

et al. 2016

Advances in Physics: X

104

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Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-ofthe-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticlemediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.

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Single-cell optoporation and transfection using femtosecond laser and optical tweezers

Cited by 69 publications

References 46 publications

Cell mechanics in biomedical cavitation

Cell mechanics in biomedical cavitation

Viscoelasticity measurements inside liposomes

Laser-assisted photoporation: fundamentals, technological advances and applications

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