Single-Cell Omics in Crop Plants: Opportunities and Challenges

Kalia, Anu; Sharma, Sat Pal

doi:10.1016/b978-0-12-817532-3.00020-7

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…Omics studies are well -elaborated in plant and human system, however, little is known in microalgae. [195,196] Table 2 summarizes the transcriptomics and proteomics studies related to product formation in microalgae strains. The oleaginous microalgae C. vulgaris is well known for the overproduction of lipids, promoting enhanced biofuel production.…”

Section: Omics Approaches For Enhancement Of Biorefinery Capabilities...mentioning

confidence: 99%

Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review

Singh

Shourie

Mazahar

2022

CLEAN Soil Air Water

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Bioremediation of wastewater using microalgae is inexpensive, energy efficient, and effective in pollutant reduction as compared to conventional wastewater treatment technologies. Wastewater is a huge resource of minerals, nutrients, bioenergy, and valuable organic compounds and can be used for cultivation of microalgae. The microalgal biomass can be further used as biorefinery feedstock to produce biofuels and commercially important high‐value products. The potential of microalgae toward bioremediation and biorefinery applications presents the avenues for integrating the two processes to support circular bioeconomy and sustainability. This review presents a holistic view of integration of bioremediation and biorefinery processes using microalgae for deriving multiple benefits like pollutant removal, resource recovery, biofuel production, and generation of high‐value commercial products. The current status of high‐throughput microalgal screening technologies is also discussed since the selection of suitable microalgal strains is crucial for the application. The review further summarizes various processes involved in bioremediation and biorefinery systems such as cultivation, bioremediation, harvesting, and downstream processing. Recent trends in microalgal strain improvement for bioremediation and biorefinery applications through genetic engineering, bioinformatics, omics technologies, and genome editing tools are highlighted, while addressing the risks, biosafety issues, and regulatory affairs associated with genetically modified algae.

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Section: Omics Approaches For Enhancement Of Biorefinery Capabilities...mentioning

confidence: 99%

Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review

Singh

Shourie

Mazahar

2022

CLEAN Soil Air Water

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“…Furthermore, it would also be possible to design a system to increase the production of the targeting material. Much of the integrated research is done in human and plant cells, but relatively little are done in microalgae [ 67 , 68 ]. Nonetheless, this combination of omics would be an important and feasible tool for glycobiological products from microalgae.…”

Section: Research Challenges and Future Perspectivesmentioning

confidence: 99%

Algal glycobiotechnology: omics approaches for strain improvement

et al. 2021

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Microalgae has the capability to replace petroleum-based fuels and is a promising option as an energy feedstock because of its fast growth, high photosynthetic capacity and remarkable ability to store energy reserve molecules in the form of lipids and starch. But the commercialization of microalgae based product is difficult due to its high processing cost and low productivity. Higher accumulation of these molecules may help to cut the processing cost. There are several reports on the use of various omics techniques to improve the strains of microalgae for increasing the productivity of desired products. To effectively use these techniques, it is important that the glycobiology of microalgae is associated to omics approaches to essentially give rise to the field of algal glycobiotechnology. In the past few decades, lot of work has been done to improve the strain of various microalgae such as Chlorella, Chlamydomonas reinhardtii, Botryococcus braunii etc., through genome sequencing and metabolic engineering with major focus on significantly increasing the productivity of biofuels, biopolymers, pigments and other products. The advancements in algae glycobiotechnology have highly significant role to play in innovation and new developments for the production algae-derived products as above. It would be highly desirable to understand the basic biology of the products derived using -omics technology together with biochemistry and biotechnology. This review discusses the potential of different omic techniques (genomics, transcriptomics, proteomics, metabolomics) to improve the yield of desired products through algal strain manipulation.

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“…So far, plant omics data have been collected at the organ or tissue level, resulting in the molecular, metabolic, and biochemical information averaged over a population of heterogeneous cells [ 160 , 161 ]. Because plant cellular processes vary spatially, single-cell multiomics is necessary for simultaneous analysis of different biomolecules to achieve accurate assessment of nodes and edges in the gene/metabolite networks operating in an individual cell [ 162 ]. Single-cell technologies have evolved intensively in the last decade [ 163 ], and transcriptomics and proteomics are viable at the single-cell level [ 164 , 165 ].…”

Section: Technical Approaches For Plant Biosystems Designmentioning

confidence: 99%

Plant Biosystems Design Research Roadmap 1.0

et al. 2020

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Human life intimately depends on plants for food, biomaterials, health, energy, and a sustainable environment. Various plants have been genetically improved mostly through breeding, along with limited modification via genetic engineering, yet they are still not able to meet the ever-increasing needs, in terms of both quantity and quality, resulting from the rapid increase in world population and expected standards of living. A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches. This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems. Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes. From this perspective, we present a comprehensive roadmap of plant biosystems design covering theories, principles, and technical methods, along with potential applications in basic and applied plant biology research. We highlight current challenges, future opportunities, and research priorities, along with a framework for international collaboration, towards rapid advancement of this emerging interdisciplinary area of research. Finally, we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception, trust, and acceptance.

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Single-Cell Omics in Crop Plants: Opportunities and Challenges

Cited by 4 publications

References 36 publications

Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review

Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review

Algal glycobiotechnology: omics approaches for strain improvement

Plant Biosystems Design Research Roadmap 1.0

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