Single cell oil production and modification for fuel and food applications: Assessing the potential of sugarcane molasses as culture medium for filamentous fungus

Bento, Heitor B.S.; Carvalho, Ana Karine Furtado de; Reis, Cristiano E. Rodrigues; Castro, Heizir F. de

doi:10.1016/j.indcrop.2020.112141

Cited by 27 publications

(25 citation statements)

References 24 publications

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“…More recently, sugarcane molasses was successfully used as sole carbon source to produce SCOs, using the filamentous fungus Mucor circinelloides . A maximum lipid productivity of 0.66g/L.d, which was equivalent to a lipid content of 29% (w/w) was attained ( Bento et al, 2020 ). In a similar study, André et al (2010) demonstrated an innovative approach of converting the biodiesel derived waste glycerol into value-added compounds (biomass, SCOs, and oxalic acid) using two fungal strains ( Lentinula edodes and Aspergillus niger ).…”

Section: Advances In Improving Sco Production In Oleaginous Microorgamentioning

confidence: 99%

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

et al. 2021

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Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

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Section: Advances In Improving Sco Production In Oleaginous Microorgamentioning

confidence: 99%

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

et al. 2021

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“…SM are broadly produced in Brazil, as well as in other countries such as India, Thailand, China and Pakistan, and are used as raw materials or as rich supplements in different segments inside and outside of biorefineries [ 17 , 18 , 19 ]. The composition of SM resembles that of sugarcane juice and, after sugar processing (sucrose crystallization), still presents a high concentration of sucrose (non-crystallized), glucose, fructose and also minerals, vitamins and trace elements [ 18 ]. Thus, being low-cost raw materials, SM could be applied as new inputs in biorefineries, contributing to the integration of processes.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, being low-cost raw materials, SM could be applied as new inputs in biorefineries, contributing to the integration of processes. Oleaginous fungus and oleaginous microalgal biomass, also named Single Cell Oil (SCO), are a very interesting product, since microbial oil may be a good alternative in the liquid fuel production chain [ 18 , 20 ]. Sugarcane molasses have been successfully assessed by different researchers as supplements to be added to other compounds or to be used by themselves as the main components in the production of SCO [ 18 , 20 , 21 ], especially in processes involving the fungal species Mucor circinelloides URM 4182 and the freshwater Scenedesmus obliquus , and they were also chosen for this study due to their robustness, with characteristics that are conducive to easy growth and maintenance, as well as being good producers of SCO [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Oleaginous fungus and oleaginous microalgal biomass, also named Single Cell Oil (SCO), are a very interesting product, since microbial oil may be a good alternative in the liquid fuel production chain [ 18 , 20 ]. Sugarcane molasses have been successfully assessed by different researchers as supplements to be added to other compounds or to be used by themselves as the main components in the production of SCO [ 18 , 20 , 21 ], especially in processes involving the fungal species Mucor circinelloides URM 4182 and the freshwater Scenedesmus obliquus , and they were also chosen for this study due to their robustness, with characteristics that are conducive to easy growth and maintenance, as well as being good producers of SCO [ 22 , 23 , 24 ]. The use of SM as carbon sources in microbial processes may contribute to the feasibility and establishment of complex biorefineries, since they are a low-cost by-product from the well-established global sugarcane industry [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

et al. 2022

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In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of Scenedesmus obliquus adhering to the mycelium of filamentous fungal Mucor circinelloides. This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores’ incubation, a culture medium containing only 2 g·L−1 of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells’ incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications.

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“…Molasses is often directly used as a feed supplement or fermented to bioproducts such as ethanol and microbial oils (Bento et al 2020;Mordenti et al 2021). Molasses was also studied for producing transfructosylating enzymes, but the studies were only limited to Aspergillus strains (Dorta et al 2006;Xie et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Production of Transfructosylating Enzymes Using Low-Cost Sugarcane Molasses by A. Pullulans FRR 5284

Khatun

Hassanpour

Harrison

et al. 2021

Preprint

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In this study, sugarcane molasses was used to produce transfructosylating enzymes by A. pullulans FRR 5284. It was found that NaNO3 was a better nitrogen source than yeast extract while exogeneous phosphorous was not needed. Adding only 4.4 g/L NaNO3 into the molasses medium containing 100 g/L sugars led to the highest total transfructosylating activity of 123.8 U/mL. Scale-up of the enzyme production process from shake flasks to 1 L reactor improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than the corresponding activity and productivity from shake flasks, respectively. FOS production from 500 g/L sucrose led to the highest yields of ~ 61% using intracellular, extracellular, and total enzymes from shake flasks and the reactor. Enzymes from different sources led to very different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios to adjust prebiotic activity.

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Single cell oil production and modification for fuel and food applications: Assessing the potential of sugarcane molasses as culture medium for filamentous fungus

Cited by 27 publications

References 24 publications

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

Use of Fungal Mycelium as Biosupport in the Formation of Lichen-like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile

Highly Efficient Production of Transfructosylating Enzymes Using Low-Cost Sugarcane Molasses by A. Pullulans FRR 5284

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