Single cell mass spectrometry studies reveal metabolomic features and potential mechanisms of drug-resistant cancer cell lines

Sun, Mei; Chen, Xingxiu; Yang, Zhibo

doi:10.1016/j.aca.2022.339761

Cited by 15 publications

(15 citation statements)

References 105 publications

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“…Multidrug resistance refers to the simultaneous resistance of tumor cells to various drugs with different structures and chemotherapy mechanisms [ 4 ]. Mechanisms of drug resistance have been reported, including (1) activation of the drug target enzyme DNA topoisomerase N or detoxifying enzyme glutathione S-transferase [ 19 ]; (2) DNA self-repair [ 20 ]; and (3) apoptosis resistance of cancer cells and changes in some signaling pathways [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

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Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway

Song

Yao

Wang

et al. 2022

Applied Bionics and Biomechanics

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Background. Multidrug resistance is the main cause of tumor recurrence and metastasis. Therefore, it is urgent to explore the mechanism and treatment of drug resistance of tumor cells. We aim to investigate the relationship between drug resistance and angiogenesis in SW480 colon cancer cells and the possible underlying mechanism. Methods. Exosomes were extracted from SW480-sensitive or SW480-resistant colon cancer cells (SW480/oxaliplatin). The CCK-8 assay, migration assay, tube formation assay, qPCR, and Western blotting were performed in human umbilical vein endothelial cells (HUVECs). The underlying mechanisms were detected by Western blotting assays and BMP-2 si-RNA silencing assay in vitro and in vivo. Results. The conditioned medium and exosomes of SW480/oxaliplatin cells promoted proliferation, migration, and tube formation of HUVECs. The expression of BMP-2 released by SW480/oxaliplatin exosomes was 2.3-folds higher than that by SW480 exosomes. Additionally, exosomal BMP-2 inhibiting the Smad signaling pathway induced the expression of vascular endothelial growth factor and CD31. Silencing of BMP-2 partly blocks the promoting effect of SW480/oxaliplatin exosomes on angiogenesis. Moreover, SW480/oxaliplatin cells increased the BMP-2 expression, consequently promoting angiogenesis in vivo. Conclusions. SW480-resistant colon cancer exosomes promoted angiogenesis via the BMP-2/Smad signaling pathway, which is potential for the novel treatment for antiangiogenic therapies in colon cancer.

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Section: Discussionmentioning

confidence: 99%

“…Chemotherapy is the primary treatment for advanced metastatic tumors [ 3 ]. However, multidrug resistance (MDR) often leads to poor efficacy of chemotherapy [ 3 , 4 ]. The proliferation of drug-resistant cells is accelerated and metastases to distant regions, promoting increased angiogenesis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway

Song

Yao

Wang

et al. 2022

Applied Bionics and Biomechanics

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“…MS is also emerging as a key technology in the detection of lipids from single cells; however, there are some limitations in the sensitivity and/or specificity of currently available technologies. Commonly used ionization techniques for lipid single cell analysis include matrix-assisted laser desorption ionization (1-10), secondary ion mass spectrometry (11)(12)(13), laser ablation electrospray ionization (14,15), nanospray desorption electrospray ionization (16) and nanoelectrospray ionization (NanoESI) (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). NanoESI capillaries are an ideal size for directly sampling and infusing the contents of a single cell into the mass spectrometer, but this process is quite laborious and requires the use of micromanipulators for handling and sampling from single cells which limits its throughput.…”

Section: Introductionmentioning

confidence: 99%

“…NanoESI capillaries are an ideal size for directly sampling and infusing the contents of a single cell into the mass spectrometer, but this process is quite laborious and requires the use of micromanipulators for handling and sampling from single cells which limits its throughput. Some improvements to the throughput of the technique have been made through the use of a continuous sampling arm that uses dual-bore quartz needles to sample from cells located on a movable stage (i.e., single probe MS) (21)(22)(23)(26)(27)(28). However, the spray 4 duration per cell is limited (~3 min) which precludes the acquisition of tandem mass spectrometry data from all but the most abundant metabolites (26).…”

Section: Introductionmentioning

confidence: 99%

“…Some improvements to the throughput of the technique have been made through the use of a continuous sampling arm that uses dual-bore quartz needles to sample from cells located on a movable stage (i.e., single probe MS) (21)(22)(23)(26)(27)(28). However, the spray 4 duration per cell is limited (~3 min) which precludes the acquisition of tandem mass spectrometry data from all but the most abundant metabolites (26). Improvements in spray duration from a single cell have been made by reducing the flow of analyte into the instrument (i.e., picoESI) (24).…”

Section: Introductionmentioning

confidence: 99%

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FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins

Hancock

Ding

Beves

et al. 2022

Preprint

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Recent advances in single-cell genomics and transcriptomics technologies have transformed our understanding of cellular heterogeneity in growth, development, ageing and disease. Despite these advances, however, single-cell metabolite profiling (i.e., metabolomics) methods have comparatively lagged behind due to limitations in technology. We have developed a high-throughput method for the detection and quantification of a wide range of phosphatidylcholine (PC) and sphingomyelin (SM) species from single cells that combines fluorescence-assisted cell sorting (FACS) with automated chip-based nanoelectrospray ionisation (nanoESI) and shotgun lipidomics. We show herein that our method is capable of quantifying more than 50 different PC and SM species from single cells and can easily distinguish between cells of different lineages or cells treated with exogenous fatty acids. Moreover, our method can detect more subtle differences in the lipidome between cell lines of the same cancer type. Our approach has the capacity to be run in parallel with other single-cell technologies to deliver near-complete multi-omics data on cells with a similar phenotype and has the capacity to significantly advance our current knowledge on cellular heterogeneity.

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Omics in Precision Medicine

Kayalar

Rajabi

Mortazavi

et al. 2023

Oncology: Genomics, Precision Medicine and Therapeutic Targets

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Single cell mass spectrometry studies reveal metabolomic features and potential mechanisms of drug-resistant cancer cell lines

Cited by 15 publications

References 105 publications

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway

FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins

Omics in Precision Medicine

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