Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection

Park, Hyunjin; Kim, Dong-Il; Yun, Kwang-Seok

doi:10.1016/j.snb.2010.07.020

Cited by 66 publications

(39 citation statements)

References 34 publications

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“…This unique dielectric signature can be utilized to discriminate and identify cells from the other particles or to detect and isolate diseased or damaged cells by means of AC-DEP (DEP force spectra of different cell types can be found elsewhere [118,144]). AC-DEP has been implemented for the separation of cancer cells from blood stream [17,18], the separation of red blood cells and polystyrene particles [19], the separation of human leukocytes [20], the isolation of the malaria-infected cells from the blood [21,22], the separation of the electroporated and non-electroporated cells [23], the separation of the platelets from diluted whole blood [24], the separation of red blood cells and the white blood cells [25], the separation [26][27][28] and sorting [29] of viable and nonviable yeast cells, the separation of healthy and unhealthy oocyte cells [30], the characterization and the sorting stem cells and their differentiated progeny [31], the isolation of rare cells from biological fluids [32], the separation of three distinct bacterial clones of commonly used E. coli MC1061 strain [33], trapping of viable mammalian fibroplast cells [34], trapping of DNA molecules [35], trapping of single cancer and endothelial cells to investigate pairwise cell interactions [36], trapping of bacterial cells for the subsequent electrodisruption or electroporation [37], focusing of polystyrene particles [38], trapping of yeast cells [39], 3-D focusing of polystyrene particles and yeast cells [40], the separation of airborne bacterium, Micrococcus luteus, from a mixture with dust and polystyrene beads [41], trapping and isolation of human stem cell from heterogeneous solution [42], single-cell isolation [43], concentration and counting of polystyrene particles [44], the separation of polystyrene particles, Jurkat cells and HeLa cells …”

Section: Applications Of Dep In Microfluidicsmentioning

confidence: 99%

Dielectrophoresis in microfluidics technology

2011

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Dielectrophoresis (DEP) is the movement of a particle in a non-uniform electric field due to the interaction of the particle's dipole and spatial gradient of the electric field. DEP is a subtle solution to manipulate particles and cells at microscale due to its favorable scaling for the reduced size of the system. DEP has been utilized for many applications in microfluidic systems. In this review, a detailed analysis of the modeling of DEP-based manipulation of the particles is provided, and the recent applications regarding the particle manipulation in microfluidic systems (mainly the published works between 2007 and 2010) are presented.

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Section: Applications Of Dep In Microfluidicsmentioning

confidence: 99%

Dielectrophoresis in microfluidics technology

2011

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“…One major benefit of DEP is that it can be used to precisely capture and position single cells for analysis. Park et al [71] used pDEP to trap HeLa cells atop a linear array of functionalized micromachined cantilevers. The cells were cultured on the cantilever and the mass of an individual HeLa cell was measured based on the resonant frequency shift of the cantilever.…”

Section: Dielectrophoretic Cellular Manipulationmentioning

confidence: 99%

“…Park et al [71] integrated impedance sensing with DEP to monitor the single-cell trapping of cancerous human breast epithelial cells (MCF7). As shown in Fig.…”

Section: Dielectrophoretic Cellular Manipulationmentioning

confidence: 99%

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

2011

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Over the past decade, dielectrophoresis (DEP) has evolved into a powerful, robust and flexible method for cellular characterization, manipulation, separation and cell patterning. It is a field with widely varying disciplines, as it is quite common to see DEP integrated with a host of applications including microfluidics, impedance spectroscopy, tissue engineering, real-time PCR, immunoassays, stem-cell characterization, gene transfection and electroporation, just to name a few. The field is finally at the point where analytical and numerical polarization models can be used to adequately describe and characterize the dielectrophoretic behavior of cells, and there is ever increasing evidence demonstrating that electric fields can safely be used to manipulate cells without harm. As such, DEP is slowly making its way into the biological sciences. Today, DEP is being used to manipulate individual cells to specific regions of space for single-cell assays. DEP is able to separate rare cells from a heterogeneous cell suspension, where isolated cells can then be characterized and dynamically studied using nothing more than electric fields. However, there is need for a critical report to integrate the many new features of DEP for cellular applications. Here, a review of the basic theory and current applications of DEP, specifically for cells, is presented.

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“…A few years later, the authors designed a device of phototransistor-based OETs for Fig. 6 (a) Schematic diagram of proposed microfluidic chip; (b) Forces act on a particle, causing the particle moving into the trapping chamber for analyzing [41].…”

Section: Cell Dispenser Hydrophobic Coatingmentioning

confidence: 99%

Single Cell Manipulation Technology

Lin¹,

Chen²,

Xie³

et al. 2015

Nano BioMed ENG

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With microfluidic technique emerging, cell manipulation technology combines with microfluidic become a promising tool for single-cell-level manipulation. To obtain a kind of or single pure target cell for eliminating interference of useless cells in the trial of molecular biology, genetic analysis, proteomics and single cell analysis, various cell manipulation techniques have been developed for recovery specific cells. In this review, we introduce the principles of each cell manipulation technology and overlook the latest achievements of cell manipulation technique by categorizing externally applied manipulation forces: optical, electrical, magnetic, acoustic, mechanical. We also summarize the advantages and drawbacks of each cell manipulation technique.

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Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection

Cited by 66 publications

References 34 publications

Dielectrophoresis in microfluidics technology

Dielectrophoresis in microfluidics technology

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

Single Cell Manipulation Technology

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