Single Cell Manipulation Technology

Lin, Shujing; Chen, Di; Xie, Yao; Zhi, Xiaoqin; Wei, Qian; Pan, Fei; Cui, Daxiang

doi:10.5101/nbe.v7i3.p75-91

Cited by 1 publication

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References 74 publications

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“…As a technology, microfluidics offers many advantages, such as very small consumption of samples and reagents, high resolution and sensitivity, low cost, and significant reduction in assay time . Because of these advantages, microfluidics has been successfully applied in the biomedical field, for example, in particle separation, cell separation, DNA/RNA manipulation, polymerase chain reaction (PCR), detection of circulating tumor cells (CTCs) and droplet generation . In recent years, increasing attention has been paid to the development of new exosome separation and detection techniques based on microfluidics .…”

Section: Introductionmentioning

confidence: 99%

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have revealed that exosomes play a significant role in intercellular communication and are closely associated with the pathogenesis of disease. Therefore, exosomes are considered promising biomarkers for disease diagnosis. However, exosomes are always mixed with other components of body fluids. Consequently, separation methods for exosomes that allow high‐purity and high‐throughput separation with a high recovery rate and detection techniques for exosomes that are rapid, highly sensitive, highly specific, and have a low detection limit are indispensable for diagnostic applications. For decades, many exosome separation and detection techniques have been developed to achieve the aforementioned goals. However, in most cases, these two techniques are performed separately, which increases operation complexity, time consumption, and cost. The emergence of microfluidics offers a promising way to integrate exosome separation and detection functions into a single chip. Herein, an overview of conventional and microfluidics‐based techniques for exosome separation and detection is presented. Moreover, the advantages and drawbacks of these techniques are compared.

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Section: Introductionmentioning

confidence: 99%