Single Capillary Isotachophoresis--Zone Electrophoresis: Current Practice and Prospects, A Review

Mazereeuw, M.; Tjaden, U.R.; Reinhoud, N.J.

doi:10.1093/chromsci/33.12.686

Cited by 52 publications

(33 citation statements)

References 3 publications

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“…Analytes dissolved in Ringer's solution cannot be detected with normal CE due to sample destacking, and therefore were dissolved in BGE for comparison. Theoretical peak widths based on longitudinal diffusion only were calculated using equation 1 (1) where W ½ ,diff is the width at half the peak height, D is the diffusion coefficient for small molecules, and t m is the migration time (s) in normal CE. Peak width calculations were made using a diffusion coefficient range of 1 × 10 −6 to 5 × 10 −5 cm 2 ·s −1 , typical for small organic molecules [ 21 ].…”

Section: Enhanced Ph-mediated Stacking With a Dynamic Ph Junction Of mentioning

confidence: 99%

“…This can be especially detrimental when using photometric detection since the small capillary dimensions already place a limitation on concentration sensitivity. Improving sensitivity for optical detection methods has been the focus of many investigations [1][2][3][4]. The most common method to reverse this effect is to perform a dilution or extraction of the sample into a low-ionic strength matrix in order to increase the field strength in the sample zone relative to the background electrolyte (BGE), and is generally referred to as fieldamplified sample stacking [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced pH‐mediated stacking of anions for CE incorporating a dynamic pH junction

Arnett

Lunte

2007

Electrophoresis

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A technique has been developed to enhance analyte focusing for capillary electrophoresis for the analysis of physiological samples. High-ionic strength samples are titrated to low-ionic strength online using pH-mediated sample stacking in conjunction with a dynamic pH junction. This method concentrates analytes by reducing their electrophoretic mobility during field-amplification. Parameters responsible for enhanced focusing were investigated, and an enhanced pH-mediated stacking method was optimized for anionic nucleosides. The process results in ultra-narrow peak widths, for example, 0.28 s for thymidine with a 10-minute analysis time. Peak width and resolution with the enhanced stacking method were also compared to normal base stacking and electrokinetic injection. With this technique, mass-loading capacity can be increased without degradation in peak shape and resolution is dramatically improved.

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Section: Enhanced Ph-mediated Stacking With a Dynamic Ph Junction Of mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced pH‐mediated stacking of anions for CE incorporating a dynamic pH junction

Arnett

Lunte

2007

Electrophoresis

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“…22 In this method, the capillary is Ðrst Ðlled with a high-mobility background electrolyte, followed by the sample with lower mobility and Ðnally a terminating electrolyte with mobility lower than that of the sample. As a result of the discontinuous bu †er system, the Ðeld strength varies along the capillary.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of adsorption preconcentration/capillary zone electrophoresis/nanoelectrospray mass spectrometry for peptide and glycoprotein analyses

1998

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“…This can be especially detrimental when using UV detection since the small capillary dimensions already place a limitation on concentration sensitivity. There have been several strategies developed to improve the concentration sensitivity of CE on-line, including field-amplified sample stacking [7][8][9][10][11], large-volume sample stacking [12,13], isotachophoresis [14,15], stacking of neutral analytes [16,17], dynamic pH junction [18,19], the transient moving chemical reaction boundary method (tMCRBM) [20,21], and chromatographic preconcentration [22]. Field-amplified stacking is the simplest of these techniques and has therefore been the most commonly used approach for on-column pre-concentration.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the mechanism of pH‐mediated stacking of anions for the analysis of physiological samples by capillary electrophoresis

Arnett

Lunte

2003

Electrophoresis

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Capillary electrophoresis has been widely used for the analysis of physiological samples such as plasma and microdialysate. However, sample destacking can occur during the analysis of these highionic strength samples, resulting in poor separation efficiency and reduced sensitivity. A technique termed pH-mediated stacking of anions (base stacking) has previously been developed to analyze microdialysate samples and achieve on-line preconcentration of analytes by following sample injection with an injection of sodium hydroxide. In this work, the mechanism of base stacking was investigated. Peak efficiency was shown to be a function of background electrolyte and sample ionic strength. Analytes representing several classes of compounds with a wide range of mobilities were used to study the effects of multiple parameters on sample stacking. The length of hydroxide injection required for stacking was shown to be dependent on analyte mobility and the type of amine background electrolyte used. Combinations of electrokinetic and hydrodynamic injections of sample and hydroxide were examined and it was concluded that although stacking could be achieved with several injection modes, electrokinetic injection of both sample and hydroxide was most effective for sample stacking. The mechanism of pH-mediated stacking for each of these modes is presented.

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Single Capillary Isotachophoresis--Zone Electrophoresis: Current Practice and Prospects, A Review

Cited by 52 publications

References 3 publications

Enhanced pH‐mediated stacking of anions for CE incorporating a dynamic pH junction

Enhanced pH‐mediated stacking of anions for CE incorporating a dynamic pH junction

Evaluation of adsorption preconcentration/capillary zone electrophoresis/nanoelectrospray mass spectrometry for peptide and glycoprotein analyses

Investigation of the mechanism of pH‐mediated stacking of anions for the analysis of physiological samples by capillary electrophoresis

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