Single-breath nitric oxide measurements in asthmatic patients and smokers

Persson, Mats A. A.; Gustafsson, Lars E.; Zetterström, Olle; Agrenius, Veronica; Ihre, E.

doi:10.1016/s0140-6736(94)90935-0

Cited by 461 publications

(268 citation statements)

References 8 publications

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“…Several investigators [24,25] demonstrated that smoking could reduce endogenous NO production. In this study, there were no significant differences in NO metabolite level in the induced sputum between smokers and nonsmokers in asthmatic subjects as well as in normal controls.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide metabolites in induced sputum: a marker of airway inflammation in asthmatic subjects

Choi²,

et al. 1999

Clin Experimental Allergy

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SummaryBackground and objective The role of nitric oxide (NO) needs to be further clarified in allergic inflammation. This study was designed to investigate the relationships between NO metabolites and eosinophil count, eosinophil cationic protein (ECP), interleukin (IL)-5 in induced sputum from asthmatics. Methods Hypertonic saline-induced sputum was obtained in 25 asthmatic subjects, among which 13 patients were examined before and after anti-asthmatic medications including steroid preparations. Ten normal subjects were enrolled as controls. Fresh expectorated sputum separated from saliva was treated with equal volume of dithiothreitol 0.1%, cytospined for cell count, and the supernatant was collected for biochemical assay. NO metabolites were assayed by using modified Griess reaction. ECP was measured by fluoroimmunoassay, and detected IL-5 by a sandwich ELISA. Results Asthmatic subjects, compared with controls, had significantly higher concentration of NO metabolites (1035.4 Ϯ 125.3 vs 557.2 Ϯ 101.5 mmol/L, P < 0.01), higher percentage of eosinophils (25.6 Ϯ 4.6 vs 1.7 Ϯ 0.2%, P < 0.01), and higher levels of ECP (1117.8 Ϯ 213.9 vs 154.6 Ϯ 47.4 mg/L, P < 0.01) in the induced sputum. IL-5 was detected more frequently in asthmatic subjects than in control subjects (11/25 [44%] vs 1/10 [10%], P < 0.05). According to asthma severity, moderate to severe asthmatic subjects (n ¼ 18) had higher level of NO metabolites (1143.8 Ϯ 156.3 vs 575.5 Ϯ 89.5 mmol/L, P < 0.01), higher levels of ECP and IL-5 (P < 0.01, respectively) in the induced sputum than in those of mild asthmatic subjects (n ¼ 7). NO metabolites, the percentage of eosinophils, the levels of ECP, and IL-5 were reduced following treatment with anti-asthmatic drugs (P < 0.01, respectively). There were significant positive correlations between NO metabolites and percentage of eosinophils or ECP (r ¼ 0.34, P < 0.05; r ¼ 0.28, P < 0.05). Negative correlations were noted between FEV 1 , FEV 1 /FVC and proportion of eosinophils, ECP, or IL-5 levels. Conclusion These findings confirmed that the level of NO metabolites was increased in the tracheobronchial secretion of asthmatic subjects and was paralleled with severity of asthma. Measurement of NO metabolites in induced sputum may be used for monitoring the degree of airway inflammation in asthmatics.

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Section: Discussionmentioning

confidence: 99%

Nitric oxide metabolites in induced sputum: a marker of airway inflammation in asthmatic subjects

Choi²,

et al. 1999

Clin Experimental Allergy

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“…22 A variety of cell types, including the pulmonary endothelium, are potential sources of NO in the lung. 13 Nevertheless, the level of NO during exhalation differs in various pathophysiologic conditions; for examples, it is increased in patients with asthma, 23,24 bronchiectasis, 25 systemic lupus erythematosus, 26 and liver cirrhosis. 27,28 NO levels were also found to increase with exercise, 29,30 during the mid menstrual cycle in women, 31 and during pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Reduced production of nitric oxide during haemodialysis

Sumino

Sato

Sakamaki³

et al. 1999

J Hum Hypertens

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Objective: To examine the influence of systemic nitric oxide (NO) synthesis on blood pressure in patients with chronic renal failure undergoing haemodialysis, since nitric oxides are susceptible to renal excretion or are dialysed, a different indicator that is unaffected by renal function, such as the level of exhaled NO was evaluated. We examined the levels of the endogenous NO before and after a haemodialysis session. Design and methods: We evaluated the serum concentrations of nitrite/nitrate and the rate of nitric oxide release into exhaled air in 10 patients with hypertension who were receiving maintenance haemodialysis. Results: The serum concentrations of nitrite/nitrate before haemodialysis were significantly higher than those in 10 normal controls (183 ؎ 151 M vs 42 ؎ 17 M, P Ͻ 0.05). These levels decreased signifi-

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“…Singlebreath analysis, whilst having been advocated as a simple measurement [12], is not possible with current technology. Normal subjects and the nonhypoxaemic cirrhotics had exhaled values for NO similar to earlier reports [5].…”

Section: Discussionmentioning

confidence: 99%

Elevated exhaled nitric oxide in patients with hepatopulmonary syndrome

Cremona¹,

Higenbottam²,

Mayoral³

et al. 1995

Eur Respir J

183

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E El le ev va at te ed d e ex xh ha al le ed d n ni it tr ri ic c o ox xi id de e i in n p pa at ti ie en nt ts s w wi it th hUsing a chemiluminescence analyser, we have measured endogenously produced NO in the exhaled air of three patients with the hepatopulmonary syndrome, six normoxaemic cirrhotic patients and six healthy volunteers. The subjects breathed NO-free air throughout the measurements.The molar rate of production of exhaled NO was raised almost threefold in the patients with hepatopulmonary syndrome compared with normal volunteers and with normoxaemic cirrhotic patients. Hypoxia per se, achieved in the normal volunteers by breathing a hypoxic gas mixture, reduced rather than increased the exhaled NO. One hepatopulmonary syndrome patient received an orthotopic liver transplant and achieved normoxaemia after 3 months. The exhaled NO also returned to normal.Increased pulmonary production of NO could contribute to the development of the hepatopulmonary syndrome.

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Single-breath nitric oxide measurements in asthmatic patients and smokers

Cited by 461 publications

References 8 publications

Nitric oxide metabolites in induced sputum: a marker of airway inflammation in asthmatic subjects

Nitric oxide metabolites in induced sputum: a marker of airway inflammation in asthmatic subjects

Reduced production of nitric oxide during haemodialysis

Elevated exhaled nitric oxide in patients with hepatopulmonary syndrome

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