Single and consecutive application of near-infrared and green irradiation modulates adipose derived stem cell proliferation and affect differentiation factors

Crous, Anine; Rensburg, Madeleen Jansen van; Abrahamse, Heidi

doi:10.1016/j.biochi.2021.07.009

Cited by 17 publications

(11 citation statements)

References 45 publications

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“…During the in vitro PDT treatment of lung cancer cells, the cells undergo some structural morphological changes as an effect of the PBM and PDT treatments that are applied. The morphological effects observed using PBM on noncancerous cells had shown no structural changes, however an increase in cell density had been seen [23,24]. Similar morphological outcomes were seen when treating cancerous cells with PBM using a low fluence [25].…”

Section: Morphologymentioning

confidence: 75%

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Photodynamic Therapy with an AlPcS4Cl Gold Nanoparticle Conjugate Decreases Lung Cancer’s Metastatic Potential

Crous

Abrahamse

2022

Coatings

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Cancer metastasis and the risk of secondary tumours are the leading causes of cancer related death, and despite advances in cancer treatment, lung cancer remains one of the leading causes of death worldwide. A crucial characteristic of metastases is cell invasion potential, which is mainly determined by cell motility. Photodynamic therapy (PDT), known for its minimally invasive cancer treatment approach, has been extensively researched in vitro and is currently being developed clinically. Due to their physicochemical and optical properties, gold nanoparticles have been shown to increase the effectivity of PDT by increasing the loading potential of the photosensitizer (PS) inside cancer cells, to be biocompatible and nontoxic, to provide enhanced permeability and retention, and to induce lung cancer cell death. However, effects of gold nano phototherapy on lung cancer metastasis are yet to be investigated. The aim of this in vitro study was to determine the inhibitory effects of PS-gold nano bioconjugates on lung cancer metastasis by analysing cell proliferation, migration, cell cycle analysis, and extracellular matrix cell invasion. The findings indicate that nano-mediated PDT treatment of lung cancer prevents lung cancer migration and invasion, induces cell cycle arrest, and reduces lung cancer proliferation abilities, elaborating on the efficacy of the nano-mediated PDT treatment of lung cancer.

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Section: Morphologymentioning

confidence: 75%

“…The central scratch method is a typical way of assessing cell motility in vitro [21]. The assay was performed using modified literature methods [22,23]. The migration of cells was determined using the 'central scratch' technique.…”

Section: Migrationmentioning

confidence: 99%

Photodynamic Therapy with an AlPcS4Cl Gold Nanoparticle Conjugate Decreases Lung Cancer’s Metastatic Potential

Crous

Abrahamse

2022

Coatings

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“… 41 The effectiveness of this procedure is being refined and improved continuously via diverse experimental protocols. 42 , 43 Current research has highlighted methods to prevent MSC apoptosis; to improve their survival, adhesion and homing abilities; and alter the cytokines/chemokines, microRNA (miRNA) and other homeostatic mediators that they secrete. 16 , 44 , 45 Until now, different approaches have been employed to precondition these multipotent cells: three-dimensional (3D) cell culture techniques, growth factors or transfection with specific DNA plasmid(s) and cytokine/chemokine pre-treatments showed promising results.…”

Section: The Operational Agenda Of Mscsmentioning

confidence: 99%

Steering the multipotent mesenchymal cells towards an anti-inflammatory and osteogenic bias via photobiomodulation therapy: How to kill two birds with one stone

et al. 2022

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The bone marrow-derived multipotent mesenchymal cells (MSCs) have captured scientific interest due to their multi-purpose features and clinical applications. The operational dimension of MSCs is not limited to the bone marrow reservoir, which exerts bone-building and niche anabolic tasks; they also meet the needs of quenching inflammation and restoring inflamed tissues. Thus, the range of MSC activities extends to conditions such as neurodegenerative diseases, immune disorders and various forms of osteopenia. Steering these cells towards becoming an effective therapeutic tool has become mandatory. Many laboratories have employed distinct strategies to improve the plasticity and secretome of MSCs. We aimed to present how photobiomodulation therapy (PBM-t) can manipulate MSCs to render them an extraordinary anti-inflammatory and osteogenic instrument. Moreover, we discuss the outcomes of different PBM-t protocols on MSCs, concluding with some perplexities and complexities of PBM-t in vivo but encouraging and feasible in vitro solutions.

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“…Furthermore, green laser irradiation has been shown to aid in the triggering of differentiation transcription factors [ 16 ]. While blue and green laser irradiation does not significantly stimulate proliferation, it has been shown in numerous studies that red and NIR laser light, such as 660 nm and 810 nm, upregulates proliferation significantly in ADSCs [ 8 , 9 , 17 , 18 ]. Near-infrared laser irradiation increases the homing abilities of ADSCs to injured sites by significantly increasing the migration of ADSCs [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…While blue and green laser irradiation does not significantly stimulate proliferation, it has been shown in numerous studies that red and NIR laser light, such as 660 nm and 810 nm, upregulates proliferation significantly in ADSCs [ 8 , 9 , 17 , 18 ]. Near-infrared laser irradiation increases the homing abilities of ADSCs to injured sites by significantly increasing the migration of ADSCs [ 18 , 19 ]. The current study observed the effect on iADSC transdifferentiation into neuronal-like cells when a novel combination of consecutive laser light of 825 nm and 525 nm at 10 J/cm 2 was used.…”

Section: Introductionmentioning

confidence: 99%

Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation

Rensburg

Crous

Abrahamse

2022

Stem Cells International

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Neuronal cells can be generated from adipose-derived stem cells (ADSCs) through biological or chemical inducers. Research has shown that this process may be optimized by the introduction of laser irradiation in the form of photobiomodulation (PBM) to cells. This in vitro study is aimed at generating neuronal-like cells with inducers, chemical or biological, and at furthermore treating these transdifferentiating cells with consecutive PBM of a 525 nm green (G) laser and 825 nm near-infrared (NIR) laser light with a fluence of 10 J/cm2. Cells were exposed to induction type 1 (IT1): 3-isobutyl-1-methylxanthine (IBMX) (0.5 mM)+indomethacin (200 μM)+insulin (5 μg/ml) for 14 days, preinduced with β-mercaptoethanol (BME) (1 mM) for two days, and then incubated with IT2: β-hydroxyanisole (BHA) (100 μM)+retinoic acid (RA) (10-6 M)+epidermal growth factor (EGF) (10 ng/ml)+basic fibroblast growth factor (bFGF) (10 ng/ml) for 14 days and preinduced with β-mercaptoethanol (BME) (1 mM) for two days and then incubated with indomethacin (200 μM)+RA (1 μM)+forskolin (10 μM) for 14 days. The results were evaluated through morphological observations, viability, proliferation, and migration studies, 24 h, 48 h, and 7 days post-PBM. The protein detection of an early neuronal marker, neuron-specific enolase (NSE), and late, ciliary neurotrophic factor (CNTF), was determined with enzyme-linked immunosorbent assays (ELISAs). The genetic expression was also explored through real-time PCR. Results indicated differentiation in all experimental groups; however, cells that were preinduced showed higher proliferation and a higher differentiation rate than the group that was not preinduced. Within the preinduced groups, results indicated that cells treated with IT2 and consecutive PBM upregulated differentiation the most morphologically and physiologically.

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Single and consecutive application of near-infrared and green irradiation modulates adipose derived stem cell proliferation and affect differentiation factors

Cited by 17 publications

References 45 publications

Photodynamic Therapy with an AlPcS4Cl Gold Nanoparticle Conjugate Decreases Lung Cancer’s Metastatic Potential

Photodynamic Therapy with an AlPcS4Cl Gold Nanoparticle Conjugate Decreases Lung Cancer’s Metastatic Potential

Steering the multipotent mesenchymal cells towards an anti-inflammatory and osteogenic bias via photobiomodulation therapy: How to kill two birds with one stone

Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation

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