Single and combined effects of acetic acid, furfural, and sugars on the growth of the pentose-fermenting yeast Meyerozyma guilliermondii

Perna, Michelle dos Santos Cordeiro; Bastos, Reinaldo Gaspar; Ceccato-Antonini, Sandra Regina

doi:10.1007/s13205-018-1143-0

Cited by 20 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultures of M. guilliermondii CCT7783 using xylose or sugarcane bagasse hydrolysate as substrates produced negligible amounts of ethanol (Y p/s 0.04 at 120 hr in xylose and 0.14 at 144 hr in the hydrolysate, respectively) . This same strain growing in semi‐synthetic media containing a mixture of glucose, arabinose, xylose, and furfural, produced ethanol at yields of (Y p/s ) 0.33 in 96 hr . The results obtained for ethanol production in our work are relevant for the field of biofuels production and present M. guilliermondii as an attractive microorganism for this biotechnological application.…”

Section: Resultsmentioning

confidence: 51%

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Fabricio

Valente

Ayub

2019

Biotechnology Progress

View full text Add to dashboard Cite

We studied the biotechnological potential of the recently isolated yeast Meyerozyma guilliermondii BI281A to produce polyunsaturated fatty acids and ethanol, comparing products yields using glucose, raw glycerol from biodiesel synthesis, or whey permeate as substrates. The yeast metabolism was evaluated for different C/N ratios (100:1 and 50:1). Results found that M. guilliermondii BI281A was able to assimilate all tested substrates, and the most efficient conversion obtained was observed using raw glycerol as carbon source (C/N ratio 50:1), concerning biomass formation (5.67 g·L−1) and lipid production (1.04 g·L−1), representing 18% of dry cell weight. Bioreactors experiments under pH and aeration‐controlled conditions were conducted. Obtained fatty acids were composed of ~67% of unsaturated fatty acids, distributed as palmitoleic acid (C16:1, 9.4%), oleic acid (C18:1, 47.2%), linoleic acid (C18:2 n−6, 9.6%), and linolenic acid (C18:3 n−3, 1.3%). Showing fermentative metabolism, which is unusual for oleaginous yeasts, M. guilliermondii produced 13.7 g·L−1 of ethanol (yields of 0.27) when growing on glucose medium. These results suggest the promising use of this uncommonly studied yeast to produce unsaturated fatty acids and ethanol using cheap agro‐industrial residues as substrates in bioprocess.

show abstract

Section: Resultsmentioning

confidence: 51%

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Fabricio

Valente

Ayub

2019

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Acetic acid diffuses across the cytoplasmic membrane into the cell, and the dissociation of acetic acid that occurs inside the cytosol leads to a change in the intracellular pH. The decrease in the cytosolic pH may result in cell death [ 39 – 41 ]. All eight strains of M. caribbica were able to grow on plates with the addition of 3 g/L acetic acid ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…All eight strains of M. caribbica were able to grow on plates with the addition of 3 g/L acetic acid ( Table 3 ). The study conducted by Perna et al [ 41 ] showed that M. guilliermondii CCT7783 grew on media containing L-arabinose and D-xylose in the presence of 10 g/L acetic acid and suggested that the species is capable of utilizing acetic acid as a carbon source. Charoensopharat et al [ 26 ] investigated the effect of acetic acid (4, 6, 8, and 10 g/L) incorporated in YM agar plates on yeasts isolated from Jerusalem artichoke .…”

Section: Discussionmentioning

confidence: 99%

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

Moremi

Rensburg

Grange

2020

International Journal of Microbiology

View full text Add to dashboard Cite

Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyerozyma caribbica-adapted strains were able to ferment L-arabinose to ethanol in the presence of 3 g/L acetic acid at 35°C. Meyerozyma caribbica Mu 2.2f fermented L-arabinose to produce 3.0 g/L ethanol compared to the parental strain with 1.0 g/L ethanol in the absence of acetic acid. The adapted M. caribbica Mu 2.2f strain produced 3.6 and 0.8 g/L ethanol on L-arabinose and D-xylose, respectively, in the presence of acetic acid while the parental strain failed to grow. In a bioreactor, the adapted M. caribbica Mu 2.2f strain produced 5.7 g/L ethanol in the presence of 3 g/L acetic acid with an ethanol yield and productivity of 0.338 g/g and 0.158 g/L/h, respectively, at a KLa value of 3.3 h−1. The adapted strain produced 26.7 g/L L-arabitol with a yield of 0.900 g/g at a KLa value of 4.9 h−1.

show abstract

“…It has been reported that acetate and butyrate affect the growth of yeast and mold 20 [30][31]. In this study, for the medium that is supplemented with acetate, the pH was adjusted to a range of 4.8 to 5.8.…”

Section: The Effects Of Acetate and Butyrate On The Growth Of Yeasts ...mentioning

confidence: 99%

Short-chain carboxylates facilitate the counting of yeasts in Sub-high temperatureDaqu

Ren,

Xie,

Tang

et al. 2023

Preprint

View full text Add to dashboard Cite

Sub-high temperature Daqu is a traditional solid fermenting agent that produces Chinese strong-aroma Baijiu. It is abundant in various microorganisms, including bacteria, yeasts, molds, and actinomycetes, of which yeasts play a crucial role in ethanol production and flavor formation. Counting yeasts in Daqu is difficult due to the interference of molds and bacteria. Antibiotics are employed to inhibit bacterial growth, but there is no effective way to suppress molds without affecting the growth of yeasts. In this study, short-chain carboxylates (C1-C6) were added to the culture medium at various pH conditions to investigate their effects on the growth of molds and yeasts. Results showed that they have distinct inhibitory effects in a pH-and concentration-dependent manner. A few of these tested short-chain carboxylates effectively suppress mold growth in agar plates without affecting yeast growth. Herein, a simple and feasible method for improving the efficiency of yeast isolation and counting in Daqu has been proposed, which may be useful for studying yeasts from complex habitats.

show abstract

Single and combined effects of acetic acid, furfural, and sugars on the growth of the pentose-fermenting yeast Meyerozyma guilliermondii

Cited by 20 publications

References 53 publications

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

Short-chain carboxylates facilitate the counting of yeasts in Sub-high temperatureDaqu

Contact Info

Product

Resources

About