Sinapine as an active compound for inhibiting the proliferation of Caco-2 cells via downregulation of P-glycoprotein

Guo, Ying; An, Hongli; Feng, Liuxin; Liu, Qi; Wang, Sicen; Zhang, Tao

doi:10.1016/j.fct.2014.02.035

Cited by 19 publications

(14 citation statements)

References 29 publications

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“…Recently extracts of Brassica chinensis (Tsai Tsai) had exhibited strong antioxidant activity together with in vivo anti-aging properties conducted in C. elegans . Furthermore, sinapine had shown promise in the area of cancer chemotherapy where a combination of sinapine with the known anticancer agent, doxorubicin, demonstrated synergistic activity with enhanced in vitro anticancer properties against colon cancer Caco-2 cells (Guo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

et al. 2019

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“…However [19] reported that, sinapine has beneficial effects because of the chemo-preventive, anti-tumor, anti-oxidant, and anti-mutagenic effects. Concretely, sinapine can be used as an active compound to enhance the Table 2.…”

Section: Quantification Of Phenolic Compounds By Hplc Analysismentioning

confidence: 99%

Identification and Determination of Phenolic Compounds in Rapeseed Meals (Brassica napus L.)

Yang¹,

Arasu²,

Chun³

et al. 2015

JACEN

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This study aimed in the identification and quantification of phenolic compounds in different four varieties of rapeseeds (Brassica napus L.) using LC-MS and HPLC. LC-MS analysis guided to identify 12 phenolic compounds including sinapine, sinapine(4-O-8')guaiacyl, feruloyl choline(4-O-8') guaiacyl, kaempferol-3-O-sinapoyl-sophoroside-7-O-glucoside, kaempferol-3-O-β-sophoroside, cyclic spermidine-alkaloid, feruloyl choline(5-8')guaiacyl, kaempferol-sinapoyl-trihexoside,1,2-di-O-sinapoyl-gentiobiose, 1,2-di-O-sinapoyl-glucose, feruloyl choline(4-O-8')guaiacyl-di-sinapoyl, and sinapine(4-O-8')guaiacyl-di-sinapoyl, respectively. Total phenolic compounds in rapeseed meals were ranged from 38.50 (2012 "Sunmang") to 63.95 mg/g dry weight (DW) (2014 Jeju local cultivar). Main phenolic compositions were cyclic spermidine-alkaloid, kaempferol, feruloyl choline, and sinapine. Sinapine was predominant compound among all the samples ranged from 29.74 to 52.24 mg/g DW (mean 79% of total phenolic contents). This study provided the information for the variation of phenolic compounds between the varieties of rapeseeds and the cultivation periods. Furthermore, this information can be developed for bio-energy industry as a reducer of the cost of the bio-energy products.

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“…The difference between the antiproliferative activities of the black and white mustard extracts and their glucosinolates may also be ascribed to the presence of sinapine (SNP). SNP is a small molecular alkaloid extracted from the seeds of plants belonging to the cruciferous species [29]. Several studies have shown that SNP has various pharmacological activities, including antioxidant [30], and anti-inflammatory effects [31].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that SNP has various pharmacological activities, including antioxidant [30], and anti-inflammatory effects [31]. For example, SNP has inhibited the proliferation of Caco-2 cells in a dose-dependent manner and increased the cytotoxic effect of doxorubicin suppressing Pglycoprotein expression [29]. In our work, both pure SNB and the SNB present in the extract were in the form of the salt of sinalbin glucosinolate (GSB) with SNP, while pure tested SNG was the potassium salt (SNP present in black mustard extract was not stoichiometric).…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative, Proapoptotic, Antioxidant and Antimicrobial Effects of Sinapis nigra L. and Sinapis alba L. Extracts

Boscaro¹,

Boffa²,

Binello³

et al. 2018

Preprint

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High Brassicaceae consumption reduces the risk of developing several cancer types, probably due to their glucosinolate amount. Extracts from Sinapis nigra L. and Sinapis alba L. have been obtained from leaves and seeds under different conditions using ethanol/water mixtures because well accepted by food industry. The EtOH/H2O 8:2 mixture gives better yields in glucosinolate amounts from grinded seeds, mainly sinalbin in S. alba and sinigrin in S. nigra. The highest antiproliferative activity in both non-tumour and tumour cell lines was induced by S. alba seeds extract. To evaluate whether Sinapis spp effect was only due to glucosinolate content or it was influenced by the extracts’ complexity, cells were treated with extracts or glucosinolates, in the presence of myrosinase. Pure sinigrin did not modify cell proliferation, while pure sinalbin was less effective than the extract. The addition of myrosinase increased the antiproliferative effects of the S. nigra extract and sinigrin. Antiproliferative activity was correlated to MAPKs modulation, which was cell and extract-dependent. Cell-cycle analysis evidenced a proapoptotic effect of S. alba on both tumour cell lines and of S. nigra only on HCT 116. Both extracts showed good antimicrobial activity in disc diffusion tests and on ready-to-eat fresh salad. These results underline the potential effects of Sinapis spp in chemoprevention and food preservation.

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Sinapine as an active compound for inhibiting the proliferation of Caco-2 cells via downregulation of P-glycoprotein

Cited by 19 publications

References 29 publications

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

Identification and Determination of Phenolic Compounds in Rapeseed Meals (Brassica napus L.)

Antiproliferative, Proapoptotic, Antioxidant and Antimicrobial Effects of Sinapis nigra L. and Sinapis alba L. Extracts

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