Simultaneous vitality and DNA-fragmentation measurement in spermatozoa of smokers and non-smokers

Bantel, Astrid de; Fleury‐Feith, Jocelyne; Poirot, Catherine; Berthaut, Isabelle; Garcin, Camille; Landais, Paul; Ravel, Célia

doi:10.1002/cyto.b.21185

Cited by 11 publications

(7 citation statements)

References 30 publications

(39 reference statements)

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“…Previous studies reported that cigarette smoking can cause chromosomal aberration, DNA damage, and alter the DNA methylation pattern in sperms. 30,31 Several mechanisms have been hypothesized. These mechanisms were concerning the deterioration of spermatogenesis, induction of ultrastructural abnormalities, apoptosis, and abnormal epigenetic modification.…”

Section: Discussionmentioning

confidence: 99%

Semen quality and cigarette smoking in a cohort of healthy fertile men

Tang

Pan

Wang

et al. 2019

Environmental Epidemiology

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Section: Discussionmentioning

confidence: 99%

Semen quality and cigarette smoking in a cohort of healthy fertile men

Tang

Pan

Wang

et al. 2019

Environmental Epidemiology

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“…Sperm DNA damage is partly associated with male infertility [ 17 ]. The normal reproductive processes, including sperm-egg fusion, membrane fusion, chromosome combination, and embryogenesis, can only be accomplished by sperm with intact DNA [ 18 ]. Up to now, few researchers have used DFI to examine the effect of two organisms on male infertility in China.…”

Section: Introductionmentioning

confidence: 99%

Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males

Liu

Mao

Pan

et al. 2021

Reprod Biol Endocrinol

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Recent years have seen a rising incidence of male infertility, mostly caused by the decline of sperm quality. The ratio of infertile males to infertile females has escalated from 3:7 in 2013 to current 5:5, which turns male infertility into the research focus of reproductive medicine. This study aimed to clarify the effect of reproductive tract infection by ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) on the DNA integrity and routine semen parameters of infertile males. A retrospective study was performed. A total of 259 infertile males who were treated at the Andrological Laboratory Examination and Reproductive Medicine Center in our hospital were analyzed. qRT-PCR was used to examine the infection status of CT and UU. According to the eligibility criteria, we evaluated the semen parameters and biochemical data of 253 men. Based on the results of PCR, the subjects were divided into four groups: Group I (CT positive, 63 cases), Group II (UU positive, 60 cases), Group III (CT positive and UU positive, 62 cases), and Group IV (no infection, 68 cases). DNA fragmentation index (DFI), sperm count, vitality and morphology, elastase level, seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Compared to Group IV, three groups (Group I, Group II and Group III) showed difference in semen volume, proportion of sperm with normal morphology, sperm motility, progressive motility, and vitality (P < 0.05). Compared to Group IV, Group II and Group III showed difference in DFI (P < 0.05). Compared to Group IV, Group II and Group III showed difference in elastase level (P < 0.05). VCL, VSL, VAP, WOB, ROS, TM, HDS showed differences between groups of abnormal/normal WBC (*P < 0.01).UU infection significantly increased the level of seminal leukocytes only in Group II, but not in the other three groups, indicating that UU is a factor to increase the level of seminal leukocytes. Compared with the normal leukocyte group, there were significant differences in total motility, forward motility and normal sperm ratio between the two groups. The proportion of sperm with abnormal morphology (mostly in the head) showed obvious difference between groups of high and normal seminal leukocytic levels. At the same time, in this study, SCGE and SCD verified that leukocytes could damage sperm DNA by increasing ROS, which ultimately affects male fertility.

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“…De Bantel et al, (2014) No significant difference was seen in coupled vitality and DNA fragmentation analysis between infertile men who were smokers and those who were nonsmokers. Belcheva, Ivanova-Kicheva, Tzvetkova, and Marinov, (2004) Overall, there is a clear negative trend in the association of smoking and sperm DNA integrity.…”

Section: Rosiakmentioning

confidence: 93%

“…De Bantel et al, (2014) No significant difference was seen in coupled vitality and DNA fragmentation analysis between infertile men who were smokers and those who were nonsmokers.…”

Section: Artmentioning

confidence: 93%

Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

Martínez

Majzoub

2020

Andrologia

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Infertility, defined as the failure to establish a clinical pregnancy after 1 year of regular unprotected sexual intercourse, is considered a worldwide health problem (Zegers-Hochschild, Adamson, Dyer, Racowsky, & de Mouzon, 2017). Globally, 1 out of 6 couples is suffering from failure to achieve clinical pregnancy after 1 year of regular and unprotected sexual intercourse (Sharlip et al., 2002). Poor reproductive potential due to male factor is responsible for 20%-70% of all cases (Agarwal, Mulgund, Hamada, & Chyatte, 2015). Conventional semen analysis is routinely requested to determine the fertility status of men (Barratt, 2007). However, one of the drawbacks of this test is its inability to evaluate advanced sperm functions like sperm deoxyribonucleic acid (DNA) fragmentation (SDF)

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Simultaneous vitality and DNA-fragmentation measurement in spermatozoa of smokers and non-smokers

Cited by 11 publications

References 30 publications

Semen quality and cigarette smoking in a cohort of healthy fertile men

Semen quality and cigarette smoking in a cohort of healthy fertile men

Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males

Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

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