Simultaneous Uv Method Development for Determination of Rotigotine Hydrochloride and Rasagiline Mesylate

Abstract

A precise, accurate analytical UV spectroscopic method has been developed for simultaneous estimation of rotigotine hydrochloride and rasagiline mesylate by using first order derivative spectroscopy. 50 µg mL-1 solutions of both drugs were scanned in the range of 200-800 nm and the first order overlain spectra of both drugs to check zero crossing points. Rotigotine hydrochloride showed zero crossing point at 239 nm where and rasagiline mesylate showed at 273 nm. Six-point calibration curves shown linearity in 2-12 µg mL-1 concentration range for rotigotine hydrochloride and 10-60 µg mL-1 concentration range for rasagiline mesylate. Regression equation obtained for both drugs were used further for determination of concentration in accuracy studies and developed product. In bulk drugs, rasagiline mesylate showed recovery of 98.33-100.93 % and rotigotine hydrochloride showed recovery of 101-103 %. The results obtained from analysis were validated according to ICH guidelines and were found at up to the mark.

The Evolving Landscape of Colonoscopy: Recent Developments and Complication Management

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