Simultaneous UPLC–MS/MS assay for the detection of the traditional antipsychotics haloperidol, fluphenazine, perphenazine, and thiothixene in serum and plasma

Juenke, JoEtta M.; Brown, Paul I.; Urry, Francis M.; Johnson-Davis, Kamisha L.; McMillin, Gwendolyn A.

doi:10.1016/j.cca.2013.04.014

Cited by 23 publications

(13 citation statements)

References 16 publications

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“…The concentrations of drugs are predominantly determined in serum samples from patients using liquid chromatography techniques [14,[23][24][25]. Drug levels or other clinical chemical parameters may be requested by clinicians after blood collection has been completed.…”

Section: Introductionmentioning

confidence: 99%

Measurement of rivaroxaban and apixaban in serum samples of patients

Harenberg

Kramer

et al. 2014

Eur J Clin Investigation

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BackgroundThe determination of rivaroxaban and apixaban from serum samples of patients may be beneficial in specific clinical situations when additional blood sampling for plasma and thus the determination of factor Xa activity is not feasible or results are not plausible.Materials and methodsThe primary aim of this study was to compare the concentrations of rivaroxaban and apixaban in serum with those measured in plasma. Secondary aims were the performance of three different chromogenic methods and concentrations in patients on treatment with rivaroxaban 10 mg od (n = 124) or 20 mg od (n = 94) or apixaban 5 mg bid (n = 52) measured at different time.ResultsConcentrations of rivaroxaban and apixaban in serum were about 20–25% higher compared with plasma samples with a high correlation (r = 0·79775–0·94662) using all assays (all P < 0·0001). The intraclass correlation coefficients were about 0·90 for rivaroxaban and 0·55 for apixaban. Mean rivaroxaban concentrations were higher at 2 and 3 h compared with 1 and 12 h after administration measured from plasma and serum samples (all P-values < 0·05) and were not different between 1 vs. 12 h (plasma and serum).ConclusionsThe results indicate that rivaroxaban and apixaban concentrations can be determined specifically from serum samples.

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Section: Introductionmentioning

confidence: 99%

Measurement of rivaroxaban and apixaban in serum samples of patients

Harenberg

Kramer

et al. 2014

Eur J Clin Investigation

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“…Although simultaneous analysis is very useful for TDM practice, most of the published methods allow quantification of a single compound (Patil et al ., ), sometimes with respective metabolites (Song et al ., ). Multi‐analyte methods for the quantification of psychotropic drugs (Vecchione et al ., ; Ansermot et al ., ; Fisher et al ., ; Juenke et al ., ; Patteet et al ., , ) include the newer antidepressants and antipsychotics recommended for TDM (Saar et al ., ; Remane et al ., ; Fisichella et al ., ). Furthermore, analysis time can be longer than 5 min (Josefsson et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Sistik

Urinovska

Brozmanová

et al. 2015

Biomedical Chromatography

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A UPLC/MS/MS method with simple protein precipitation has been validated for the fast simultaneous analysis of agomelatine, asenapine, amisulpride, iloperidone, zotepine, melperone, ziprasidone, vilazodone, aripiprazole and its metabolite dehydro-aripiprazole in human serum. Alprenolol was applied as an internal standard. A BEH C18 (2.1 × 50 mm, 1.7 µm) column provided chromatographic separation of analytes using a binary mobile phase gradient (A, 2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v; B, 2 mmol/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v/v/v). Mass spectrometric detection was performed in the positive electrospray ionization mode and ion suppression owing to matrix effects was evaluated. The validation criteria were determined: linearity, precision, accuracy, recovery, limit of detection, limit of quantification, reproducibility and matrix effect. The concentration range was as follows: 0.25-1000 ng/mL for agomelatine; 0.25-100 ng/mL for asenapine and iloperidone; 2.5-1000 ng/mL for amisulpride, aripiprazole, vilazodone and zotepine; 2.3-924.6 ng/mL for dehydroaripiprazole; 2.2-878.4 ng/mL for melperone; and 2.2-883.5 ng/mL for ziprasidone. Limits of quantitation below a therapeutic reference range were achieved for all analytes. Intra-run precision of 0.4-5.5 %, inter-run precision of 0.6-8.2% and overall recovery of 87.9-114.1% were obtained. The validated method was successfully implemented into routine practice for therapeutic drug monitoring in our hospital.

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“…In order to determine perphenazine (PPZ) dosage in preparations and its concentration in diverse matrices such as plasma, urine, and environmental waste waters, different analytical methods including spectrophotometry, [1][2][3][4] electrochemical, [5][6][7][8] high-performance liquid chromatography (HPLC), [9][10][11][12] gas chromatography mass spectrometry (GC-MS), 13,14 and capillary electrophoresis 5,15,16 have been introduced. In most cases, separation and preconcentration of PPZ is required prior to analysis.…”

Section: Introductionmentioning

confidence: 99%

Pre-Concentration and Separation of Perphenazine Using Picric Acid Loaded α-Fe2O3 Nanoparticles: HPLC Syringe Filter as the Particles Support

Ramezani

Shekarriz

Behfar

et al. 2017

J. Braz. Chem. Soc.

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Suspension of picric acid (PA) loaded iron oxide nanoparticles (PA-IONPs) was injected on the top of a 0.2 µm HPLC (high-performance liquid chromatography) syringe filter. The immobilized nanoparticles were used as SPE (solid phase extraction) sorbent for perphenazine separation and pre-concentration. By passing perphenazine solution through the filter, a molecular complex between perphenazine and PA was formed. Perphenazine/PA complex was eluted from the filter using dichloromethane as solvent. The perphenazine contents were determined at 254 nm. Effects of the parameters such as pH, the amount of PA-IONPs, as well as flow rate, volume, and type of eluting solvents, on the separation efficiency and pre-concentration of perphenazine were investigated. The calibration curve was linear in the range of 0.5-5 ng mL -1 (Y = 0.047X + 0.018; R 2 = 0.9952) with limit of detection of 0.16 ng mL -1. The pre-concentration and enrichment factors were 8.33 and 1.88, respectively. This method was successfully applied to the determination of perphenazine residue in tap water.

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Simultaneous UPLC–MS/MS assay for the detection of the traditional antipsychotics haloperidol, fluphenazine, perphenazine, and thiothixene in serum and plasma

Cited by 23 publications

References 16 publications

Measurement of rivaroxaban and apixaban in serum samples of patients

Measurement of rivaroxaban and apixaban in serum samples of patients

Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Pre-Concentration and Separation of Perphenazine Using Picric Acid Loaded α-Fe2O3 Nanoparticles: HPLC Syringe Filter as the Particles Support

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