Simultaneous two-dimensional HPLC determination of free d-serine and d-alanine in the brain and periphery of mutant rats lacking d-amino-acid oxidase

Miyoshi, Y.; Hamase, Kenji; Okamura, Tadashi; Konno, Ryuichi; Kasai, Noriyuki; Tojo, Yosuke; Zaitsu, Kiyoshi

doi:10.1016/j.jchromb.2010.08.024

Cited by 73 publications

(65 citation statements)

References 45 publications

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“…Two-dimensional HPLC separation of the NBD-Ala enantiomers was carried out using the Nanospace HPLC apparatus (Shiseido, Tokyo, Japan) and also using the HPLC apparatus obtained from JASCO (Tokyo, Japan) according to previous reports [27,28,34,35]. Briefly, the HPLC system consisted of a degasser, two pumps, an injector/auto-sampler, a column selection unit (high pressure valve), a column oven, two fluorescence detectors and two integrators.…”

Section: Two-dimensional Hplc Analysis Of D-and L-alanine In the Humamentioning

confidence: 99%

Sleep-Awake Profile Related Circadian D-Alanine Rhythm in Human Serum and Urine

et al. 2017

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The circadian profiles of D-alanine (Ala) amounts in human serum and urine have been clarified using a two-dimensional HPLC system combining a microbore reversed-phase C18 column and a Pirkle-type enantioselective column. The two-dimensional HPLC system enables the determination of trace amounts of D-Ala in the clinical samples without severe interference from intrinsic substances in the human physiological fluids. In the volunteers having normal sleep-awake patterns, the amounts of D-Ala in the serum were high in the late evening and low in the morning. On the other hand, in the volunteers having the reversed sleep-awake patterns, the amounts of D-Ala in the serum were high in the morning and low in the nighttime. The similar circadian changes of D-Ala amounts were observed in the urine. These 24-h profiles of D-Ala are almost the same as those observed in rats and mice, suggesting that D-Ala has fundamental physiological functions related to rest-active conditions in mammals, and could also be useful as the biomarker for sleep-awake profiles.

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Section: Two-dimensional Hplc Analysis Of D-and L-alanine In the Humamentioning

confidence: 99%

Sleep-Awake Profile Related Circadian D-Alanine Rhythm in Human Serum and Urine

et al. 2017

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“…In comparison to simple HPLC, two-dimensional HPLC (2D-HPLC) methods combining reversed-phase separation (as the first dimension) and chiral separation (as the second dimension) are useful techniques for trace analysis of l-AAs and, therefore, suitable for chiral AA metabolome analysis in physiological fluids and tissues [138][139][140][141][142][143], as well as in the fermented foods and beverages. In 2014, Miyoishi et al [128] used 2D-HPLC after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) for AA analysis, by fluorescence detection, in Kurozu vinegars.…”

Section: -Dimensional Hplcmentioning

confidence: 99%

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Tanwar

Bhushan

2015

Chromatographia

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“…Using Pirkle -type enantioselective columns, such as the Sumichiral OA-2500S and OA-3200S, a variety of NBD-proteinogenic amino acids could be separated [24][25][26][30][31][32][33]. By using the quinine based anion-exchange type chiral stationary phases, enantiomers of the NBD-amino acids were also nicely separated [27,34].…”

Section: Enantioselective Separation Of Extraterrestrial Amino Acids mentioning

confidence: 99%

Enantioselective Determination of Extraterrestrial Amino Acids Using a Two-Dimensional Chiral High-Performance Liquid Chromatographic System

et al. 2014

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A two-dimensional chiral high-performance liquid chromatographic (2D-HPLC) system has been established for the analysis of extraterrestrial amino acids. As the targets, 8 chiral amino acids (alanine (Ala), valine (Val), 2-aminobutyric acid (2AB), norvaline (nVal), N-methylalanine (N-MeAla), isovaline (iVal), 3AB and 3-aminoisobutyric acid (3AIB)) and 5 non-chiral amino acids (glycine (Gly), β-Ala, γ-aminobutyric acid (GABA), sarcosine (Sar) and 2AIB) were selected. These amino acids were tagged with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and non-enantioselectively separated by a capillary monolithic ODS column in the first dimension. The target fractions were automatically introduced into the second dimension and further separated by Pirkle-type enantioselective columns. By using this system, the 2D-HPLC separation of 21 components in small particles of a carbonaceous chondrite (Yamato 791191, Antarctic CM2 meteorite) could be successfully performed, and all of the target amino acids were observed. The D/L ratios of the chiral molecules are almost 50/50 for all of the tested proteinogenic and non-proteinogenic amino acids.

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Simultaneous two-dimensional HPLC determination of free d-serine and d-alanine in the brain and periphery of mutant rats lacking d-amino-acid oxidase

Cited by 73 publications

References 45 publications

Sleep-Awake Profile Related Circadian D-Alanine Rhythm in Human Serum and Urine

Sleep-Awake Profile Related Circadian D-Alanine Rhythm in Human Serum and Urine

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Enantioselective Determination of Extraterrestrial Amino Acids Using a Two-Dimensional Chiral High-Performance Liquid Chromatographic System

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