Simultaneous TLC-densitometric analysis of catechin, pyrocatechol and quercetine in gambir block from Pesisir Selatan

Kamal, Sefrianita; Susanti, Meri; Febriyenti,; Zaini, Erizal; Hamidi, Dachriyanus

doi:10.1016/j.heliyon.2022.e08985

Cited by 3 publications

(1 citation statement)

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“…A reverse-phase HPLC column (μBondapak TM C18 Column, 125 Å, 10 μm, 3.9 × 300 mm from Waters (Milford, MA, USA) was used for analysis. The HPLC separation conditions for catechin, epicatechin, and quercetin were modified from previous studies [ 2 , 33 ]. Catechin and epicatechin were analyzed using a gradient elution of A (H 2 O containing 0.1% formic acid), B (10% CH 3 CN containing 0.1% formic acid), and C (90% CH 3 CN containing 0.1% formic acid) at a flow rate of 1 mL/min [ 1 ].…”

Section: Methodsmentioning

confidence: 99%

Role of Herbal Extracts of Catechu from Uncaria gambir in the Treatment of Chronic Diabetic Wounds

Tsai

Hsieh

et al. 2022

Pharmaceuticals

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Catechu is a dried decoction from twigs with the leaves of Uncaria gambir. Its antioxidant, anti-inflammatory, and antimicrobial activities have been previously reported because of its high catechin and epicatechin content (>21%). It is also one of the components used in traditional Chinese herbal medicine, “Jinchuang Ointment,” which has excellent efficacy in treating chronic diabetic wounds. An in vivo zebrafish embryo platform and an in vitro cell-based tube formation assay were used to measure the angiogenic activity of catechu extracts. Interestingly, for the first time, catechu extracts stimulated angiogenic activity on both platforms. The expression of the IL-8 gene was induced in HMEC1 cells after treatment with catechu extracts for 1 h only. In contrast, the upregulation of FGFR2, FGFR3, NF-κB, STAT3, and vimentin persisted for 24 h. A summary of the possible mechanisms underlying the angiogenic activity of catechu extracts in HMEC1 cells is shown. Unexpectedly, catechu extracts inhibited the migration of HaCaT cells. These results can account for the intense blood flow flux in porcine excisional wound sites in our previous studies, which provides insights into the therapeutic activity of catechu extract in chronic diabetic wounds.

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Section: Methodsmentioning

confidence: 99%