Simultaneous Study of NH⁺₄, Na⁺, Mg²⁺, Ca²⁺, NO^2-₃, SO^2-₄, (NO^-₂), H{2in}PO^-₄ and CI^- Uptake by Intact Winter Wheat Seedlings in a Single Depletion ExperimentPO^-<s

Abstract

Simultaneous uptake of NH~, Na +, Mg 2+, Ca 2+, NO~, SO 2-, (NOi), H2PO~ and C1-ions by N-limited winter wheat seedlings (Triticum aestivum L., cv. Regina) in a single depletion experiment was investigated. Individual ion species in uptake solution samples were determined using capillary isotachophoresis. The operating systems used are described in detail. Processing of obtained concentration data allow to construct time curves of individual ions concentration in uptake solution, time curves of accumulative uptake, and curves for uptake rate versus time and uptake rate versus external concentration. From these curves there is possible to reveal a more complex picture of behaviour ofindividua! ions during uptake process as well as to assess interactions among them. Attention was paid to the points of intersection of time curves of NO~ and NH~ uptake rates. These points characterized by equal uptake rates may be considered as limits for preference of given ion species under given experimental conditions.