Simultaneous stopped-flow determination of morphine and naloxone by time-resolved chemiluminescence

Pulgarı́n, José A. Murillo; Bermejo, Luisa F. García; Gallego, Jorge; García, M. Nieves Sánchez

doi:10.1016/j.talanta.2007.09.032

Cited by 37 publications

(20 citation statements)

References 21 publications

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“…Nos últimos anos, muitos grupos de pesquisas têm se dedicado ao desenvolvimento de novos métodos analíticos baseados na emissão de luminescência, dentre eles a quimilumi-nescência 16 A fluorescência nativa tem sido explorada para a quantificação de fármacos em medicamentos; com base nisto, muitos trabalhos têm sido descritos na literatura. 19,20,[58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]80,[110][111][112][113][114][115][118][119][120][121][122]124,125,129,[133][134][135] A Tabela 1 mostra os principais fármacos determinados através da fluorescência nativa em solução.…”

Section: Aplicação Da Espectroscopia De Luminescência No Campo Farmacunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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Recebido em 20/10/06; aceito em 30/11/07; publicado na web em 4/9/08 APPLICATION AND ADVANCES IN THE LUMINESCENCE SPECTROSCOPY IN PHARMACEUTICAL ANALYSES. This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo-and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.Keywords: luminescence spectroscopy; drugs; cosmetics analysis; introdução Devido ao progresso contínuo em técnicas e instrumentos, a indústria farmacêutica busca o emprego de metodologias que ofereçam maior sensibilidade, seletividade, robustez, precisão, exatidão e rapidez. Neste sentido, um grande número de equipamentos de cromatografia líquida de alta eficiência (CLAE) com diversos tipos de detecção domina este ramo industrial. Contudo, o advento da sofisticação de instrumentação automatizada de espectrômetros de luminescência poderá resultar em um substancial aumento de aplicações desta técnica instrumental na indústria farmacêutica, principalmente, no que se refere à análise diretamente na matriz do medicamento.A espectroscopia de luminescência é uma ferramenta analítica extremamente sensível e tem sido amplamente aplicada na resolução de problemas que requerem baixos limites de detecção, podendo ser facilmente encontrados na literatura muitos trabalhos mostrando análises ao nível de traços.1-6 A sensibilidade inerente a esta técnica é consideravelmente maior em comparação a outras metodologias, entre elas a espectrofotometria UV/visível, apresentando limites de detecção de até três ordens de grandeza menores, os quais normalmente se encontram na faixa de ng mL -1 , sendo conseqüência do baixo sinal de fundo que as medidas fluorescentes apresentam. Diferentemente do que ocorre no fenômeno da luminescência, nas medidas de absorbância o sinal está associado à atenuação da intensidade de um feixe de radiação por uma espécie absorvente. Nos casos onde a quantidade da espécie é pequena, esta atenuação é menor, tornando assim a detecção da espécie difícil. Outra característica importante da luminescência diz respeito à seletividade, porque espécies com rendimentos quânticos de fluorescência apreciáveis são menos comuns do que substâncias cuja fluorescência não pode ser detectada. Desta forma, moléculas fluorescentes apresentam comprimento de onda característico de excitação e/ou emissão. Adicionalmente, o comprimento de onda de excitação d...

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Section: Aplicação Da Espectroscopia De Luminescência No Campo Farmacunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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“…Various chromatographic methods such as liquid chromatography coupled with UV detection [4], gas chromatographymass spectrometry [5], thin layer chromatography [6], high [7] and ultra [8] performance liquid chromatography. Resonance light scattering [20] and time-resolved chemiluminescence [9] methods have been also used. However, these methods require expensive and bulky instruments and special sample pretreatments have to be performed before analysis.…”

Section: Introductionmentioning

confidence: 99%

Competitive voltammetric morphine immunosensor using a gold nanoparticle decorated graphene electrode

Eissa

Zourob

2017

Microchim Acta

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The authors describe a voltammetric immunosensor for morphine (MOR) that is based on a graphene screen printed electrode (GSPE) modified with gold nanoparticles (AuNP) which were electrodeposited on its surface. The gold nanoparticles were characterized by scanning electron microscopy, Xray photoelectron spectroscopy and cyclic voltammetry. Cysteamine was then self-assembled on the gold-modified electrodes via thiol interaction in order to introduce terminal amino groups to the electrode surface. The electrodes were then used to fabricate the immunosensor by covalent immobilization of antibodies against MOR. The sensor works on the basis of a competition between MOR and the morphine-bovine serum albumin (MOR-BSA) conjugate for the immobilized antibodies on the sensor surface and the resulting change in the square wave voltammetry reduction current using the hexacyanoferrate system as an electrochemical probe. The competitive immunoassay enables sensitive and selective detection of MOR in the 0.1 to 100 ng·mL −1 MOR concentration range, with a 90 pg·mL −1 detection limit. The method was applied to the determination of MOR in spiked saliva samples and showed high recoveries. We believe that this immunosensor offers a simple, rapid, sensitive and accurate MOR test.

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“…The same reagents and method have also been used for the determination of oxalate–tartrate and pyruvate–tartrate mixtures . Pulgarín et al also described a stopped flow technique for the simultaneous determination of morphine and naloxone in synthetic samples. In all of the above‐mentioned CL methods, the influence of the sample matrix at selected times should be investigated for each component to ensure that the slope of the calibration curve of one analyte is not affected by another .…”

Section: Introductionmentioning

confidence: 99%

Multiway analysis applied to time‐resolved chemiluminescence for simultaneous determination of paracetamol and codeine in pharmaceuticals

et al. 2016

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This method is based on the enhancing effect of codeine (COD) and paracetamol (PAR) on the chemiluminescence (CL) reaction of Ru(phen)3 (2+) with Ce(IV). In the batch mode, COD gives a relatively sharp peak with the highest CL intensity at 4.0 s, whereas the maximum CL intensity of the PAR appears at ~60 s after injection of Ce(IV) solution. Whole CL time profiles allowed use of the time-resolved CL data in combination with multiway calibration techniques, as multiway partial least squares (N-PLS), for the quantitative determination of both COD and PAR in binary mixtures. In this work, we found that the impact of Ce(IV) concentration on the CL intensity was different for COD and PAR. Therefore, a Ce(IV) concentration mode was added to the time and sample modes to obtain 3D data. The percent relative standard deviation (%RSD) values for 10 determinations of 1.0 × 10(-5) mol/L of COD and 1.0 × 10(-4) mol/L of PAR were 6.1% and 8.7%, respectively. The limit of detection (LOD) values (S/N = 3) were 0.9 × 10(-8) mol/L and 1.0 × 10(-6) mol/L for COD and PAR, respectively. The proposed method was successfully applied to the determination of PAR and COD in commercial pharmaceutical formulations. Acceptable recoveries (90-110%) were obtained for the quantification of these drugs in the real samples. Copyright © 2016 John Wiley & Sons, Ltd.

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Simultaneous stopped-flow determination of morphine and naloxone by time-resolved chemiluminescence

Cited by 37 publications

References 21 publications

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Competitive voltammetric morphine immunosensor using a gold nanoparticle decorated graphene electrode

Multiway analysis applied to time‐resolved chemiluminescence for simultaneous determination of paracetamol and codeine in pharmaceuticals

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