Simultaneous Spectrophotometric Determination of Paracetamol, Propyphenazone and Caffeine By Using Absorption Ratio Method

Pulungan, Ainil Fithri; Dlp., Effendy; Siti, M S

doi:10.22270/ajprd.v6i5.412

Cited by 3 publications

(2 citation statements)

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“…Sebanyak 50 gram kafein dilarutkan dengan aquades panas, lalu dimasukkan ke dalam labu ukur 50 mL agar didapatkan konsentrasi 1000 ppm. Kemudian dipipet sebanyak 2,5 mL lalu masukkan ke dalam labu ukur 25 mL lalu ditambahkan aquades hingga batas tanda sehingga memperoleh konsentrasi 100 ppm (Pulungan et al, 2018).…”

Section: Pembuatan Larutan Kafeinunclassified

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Hanifah,

Daulay,

Rahman

et al. 2024

jharma

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The Bajakah tampala tree (Spatholobus littoral hassk) is a native plant from the interior of the forests in Kalimantan, especially Central Kalimantan. This plant is a plant that stretches or grows and grows abundantly in the interior of the forest. The Bajakah plant is known to the people of Kalimantan as a medicinal plant and can cure various diseases, including diarrhea, rheumatism, and cancer. The aim of this research was to determine the alkaloid content of bajakah root extract in various concentrations. The research method was carried out experimentally, with the independent variable being an ethanol extract of bajakah roots with concentrations of 50, 70, and 96%. Meanwhile, the dependent variables are the characteristics of simplicia, the secondary metabolite content of Bajakah root ethanol extract, and the determination of the total alkaloid content of Bajakah root extract. Bajakah root simplicia extract is made with 96, 70, and 50% ethanol solvents. The parameters used in this study include water content, total ash content, acid insoluble ash content, water soluble extract content, ethanol soluble extract content, and secondary metabolite content, including alkaloids, tannins, flavonoids, saponins, steroids, and terpenoids, by conducting phytochemical screening. , making a standard caffeine solution, determining the maximum wavelength of caffeine, measuring the caffeine calibration curve, and determining the total alkaloid content of Bajakah root extract. The results of the phytochemical screening showed that Bajakah root was positive for containing alkaloid compounds, saponins, tannins, flavonoids, steroids, and terpenoids. And the results obtained from Bajakah root plants obtained from Central Kalimantan were a 96% ethanol concentration of 1.66797 ± 0.7088 mg/gram, a 70% ethanol concentration of 1.6679 ± 0.1124 mg/gram, and a 50% ethanol concentration of 0.46981 ± 1.0656 mg/gram

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Section: Pembuatan Larutan Kafeinunclassified

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Hanifah,

Daulay,

Rahman

et al. 2024

jharma

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“…Propyphenazone and caffeine were also determined in the presence of other biologically active substances using liquid chromatography with ultraviolet detection (LC/UV) [17], HPLC [18] and LC-ESI-MS [16]. Paracetamol, propyphenazone and caffeine (Scheme 1) were determined side by side using the spectrophotometric method [19,20], HPLC [20][21][22][23], RP-HPLC [22,24,25], spectrophotometric [20,26,27], liquid chromatography (LC) [20], square-wave voltametric [28], high-performance liquid chromatography with diode-array detection (HPLC-DAD) [29], normal-phase thin layer chromatography (NP-TLC) [24], pressurized planar electrochromatography [30] and RP-HPTLC [30]. Micellar electrokinetic capillary chromatography (MECC) has also been used to separate paracetamol, propyphenazone and caffeine in the presence of diclofenac [31].…”

Section: Introductionmentioning

confidence: 99%

Determination of the Active Pharmaceutical Ingredients in Saridon Tablets Using an Economical and Sensitive Thin Layer Chromatography Method Combined with Densitometry

Pyka-Pająk

2024

Analytica

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A new TLC–densitometric method has been developed for the identification and quantification of paracetamol (PA), propyphenazone (PP) and caffeine (C) in Saridon tablets using the NP-TLC technique combined with densitometry. This method allows for the simultaneous determination of PA, PP, and C in the same sample. Among all the tested chromatographic conditions, the mixture consisting of chloroform + toluene + ethyl acetate + ethanol + acetic acid (18:18:7.5:5.0:0.3, v/v/v/v/v) and a silica gel 60F254 plate proved to be the most effective for the separation of the three tested active pharmaceutical ingredients (APIs) and substances related to paracetamol. The full validation of the proposed NP-TLC method proved that it is specific, precise, accurate, robust and sensitive. The percentage content in relation to the content declared by the manufacturer was for propyphenazone 99.8%, paracetamol 101.6% and caffeine 100.8%, which was in accordance with pharmacopoeial requirements. The results presented indicate the possibility of using the developed method in the routine control of pharmaceutical preparations containing these APIs. The proposed method is economical and more sensitive compared to the previously proposed planar methods for the simultaneous determination of APIs. What is more, the presented method may be an excellent economical alternative when the HPLC method is unavailable for such a determination.

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Analisis kadar parasetamol dan kafein dalam sediaan tablet menggunakan metode spektrofotometri derivatif zero crossing

Pulungan,

Rosaldi,

Rani

2024

JPS

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Perkembangan obat dalam bentuk tablet kombinasi pada masa saat ini telah banyak mengalami peningkatan, kombinasi obat dalam suatu sediaan tablet dapat memberikan reaksi dan kemampuan yang semakin meningkat. Hal ini menjadikan kombinasi obat contohnya seperti Parasetamol dan kafein menjadi kombinasi yang efektif dalam bidang farmasi. Parasetamol menjadi obat analgetik antipiretik yang dapat dikombinasikan dengan kafein yang dapat digunakan dalam terapi dengan kombinasi antara obat tersebut. Penelitian ini bertujuan untuk mengetahui konsentrasi kombinasi parasetamol dan kafein dalam formulasi tablet dengan menggunakan metode spektrofotometri turunan silang nol. Pada penelitian ini dilakukan optimasi pelarut dengan 17 kategori pemilihan pelarut, dan asetat:metanol (30:70) dipilih menggunakan metode turunan silang nol dengan plot dA/d memanfaatkan panjang gelombang maksimum parasetamol 258 nm dan kafein 288 nm, karena ada pergeseran panjang gelombang yang sebenarnya dalam analisis ini. Untuk memvalidasi penetapan kadar metode turunan silang nol menggunakan serapan kedua dalam pelarut ace (30:70 asetat: metanol). Analisis spektrofotometri turunan zero crossing campuran parasetamol dan kafein diketahui bahwa kadar parasetamol pada tablet A sebesar 95,24 8,47%, pada tablet B sebesar 92,86 0%, dan pada tablet C sebesar 96,5 0%, sedangkan kandungan kafeinnya adalah 104,89 0%, 102,09 0%, dan 91,6 0%. Menurut USP 30 NF 25 (2007), dapat disimpulkan bahwa hasil pengujian komposisi parasetamol dan kafein dalam tablet memenuhi persyaratan.

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Simultaneous Spectrophotometric Determination of Paracetamol, Propyphenazone and Caffeine By Using Absorption Ratio Method

Cited by 3 publications

References 0 publications

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Determination of the Active Pharmaceutical Ingredients in Saridon Tablets Using an Economical and Sensitive Thin Layer Chromatography Method Combined with Densitometry

Analisis kadar parasetamol dan kafein dalam sediaan tablet menggunakan metode spektrofotometri derivatif zero crossing

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