Simultaneous Spectrophotometric Determination of Calcium and Magnesium

Young, Allen; Sweet, Thomas R.; Baker, Bertsil B.

doi:10.1021/ac60099a007

Cited by 30 publications

(12 citation statements)

References 11 publications

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“…wet weight heart sample, while the amounts of Mg and Ca were arbitrarily selected. In analyzing ventricular tissue, it was found that the Mg content was more than 10-fold that of Ca, and under these conditions the error in determining Ca by the simultaneous Eriochrome Black T method of Young et al 4 amounted to as much as 30 per cent. Currently, the Ca concentration in normal cardiac tissue is determined complexometrically by the procedure of Bachra et al 3 The precision of the method appears to be satisfactory for tissue analysis, and data on Ca concentrations in normal and infarcted heart muscle will be reported later.…”

Section: Analytical Recoverymentioning

confidence: 92%

“…Magnesium was determined by the spectrophotometric method of Young, Sweet and Baker. 4 Because of the acidity of the 1:50 diluted ashed sample, it was necessary to first adjust pH to that of the Mg water standards before addition of the piperidine buffer, alcohol and Eriochrome Black T dye.…”

Section: Methodsmentioning

confidence: 99%

“…As measured by wet weight-dry weight differences, the infarcted tissue was edematous. Tissue Ca concentrations are not reported due to the inaccuracy of the method of Young et al 4 when adapted to this problem. Discussion For the purpose of discussion, the following assumptions will be made: (1) that about 25 Gm.…”

Section: Cardiac Tissuementioning

confidence: 99%

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Electrolyte Changes in Heart Tissue and Coronary Arterial and Venous Plasma Following Coronary Occlusion

Cummings

1960

Circulation Research

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IOCHEMICAL reactions of myocardial cells to infarction are of basic interest in arrhythmia research. The purpose of the present investigation was to measure in the same dogs concentrations of K, Na, Ca and Mg in coronary sinus and arterial blood before infarction, during a period of ventricular arrhythmia following coronary artery occlusion, and in infarcted and noninfarcted heart tissue. For comparative purposes, similar analyses were made in control, sham-operated animals. MethodsMyocardial infarction was produced by the method of Harris 1 which consisted of ligating the left anterior descending coronary artery in 2 stages. The resultant ventricular arrhythmia, •which begins 5 to 6 hours following occlusion with the appearance of scattered ectopic beats and then develops into a nearly complete ventricular tachycardia 10 to 15 hours postligation, has been described by Clark and Cummings.2 For controls, similar experiments were carried out on shamoperated animals in which ligatures were merely placed under the coronary artery.Blood samples for hematocrit and plasma electrolyte determinations were obtained by direct puncture of the coronary sinus and the common carotid artery. At the start of the coronary occlusion and sham-ligation experiments, 2 control plasma samples were collected 30 minutes apart. Then, beginning 8 hours after ligature placement, additional plasma samples were obtained at hourly intervals. An 8 to 11 hour postligation collection

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Section: Analytical Recoverymentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Electrolyte Changes in Heart Tissue and Coronary Arterial and Venous Plasma Following Coronary Occlusion

Cummings

1960

Circulation Research

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“…These reagents are fairly sensitive but, like most such lake dyes, require extremely close attention to experimental details for reliable results. Eriochrome Black T has been used for the determination of magnesium (4, 9, 22,23,32) and for the simultaneous determination of calcium and magnesium (32). A recently reported reagent for magnesium, Magon (1,16,17), is sensitive and forms a fairly stable complex with magnesium.…”

mentioning

confidence: 99%

Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo III.

Ferguson¹,

Richard²,

O'Laughlin³

et al. 1964

Anal. Chem.

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15 431 448 20 431 431 acetic acid-chloroform mixture.In almost all cases, 5 ml. of the solvents tested were miscible with 20 ml. of acetic acid-chloroform.Recovery of peroxide was quantitative when as little as 20% by volume of acetic acidchloroform was present in the solvent mixture. It should therefore be possible to determine peroxide groups on polymers and other organic solids if the solution of the material is compatible with the minimum amount of acetic acid-chloroform required for the reaction of the peroxide with iodide.Attempts to determine di-ferf-butyl peroxide by using HC1 to increase the acidity were unsuccessful. Ten milligrams of this peroxide gave a pale yellow 2 hours after solution in 1 to 1 HC1 and the addition of KI.

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“…For complexes of widely different stability constants, it is possible to find a pH at which one complex is dissociated while the other is not. This has been made the basis of a simultaneous spectrophotometric determination of zinc and copper (11) and of calcium and magnesium ions (9,12).…”

Section: Literaturementioning

confidence: 99%

Indirect Spectrophotometric Determination of Zinc and Cobalt Using Eriochrome Blue Black R.

Rogers¹

1962

Anal. Chem.

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Simultaneous Spectrophotometric Determination of Calcium and Magnesium

Cited by 30 publications

References 11 publications

Electrolyte Changes in Heart Tissue and Coronary Arterial and Venous Plasma Following Coronary Occlusion

Electrolyte Changes in Heart Tissue and Coronary Arterial and Venous Plasma Following Coronary Occlusion

Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo III.

Indirect Spectrophotometric Determination of Zinc and Cobalt Using Eriochrome Blue Black R.

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