Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo III.

Ferguson, J.; Richard, John J.; O'Laughlin, Jerome W.; Banks, Charles V.

doi:10.1021/ac60210a028

Cited by 62 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serum was subjected to calcium and Pi measurements using the automated clinical chemistry analyzer Fuji Dri-chem 4000 (Fujifilm, Tokyo, Japan) based on colometric analysis using the chlorophosphonazo-III and purine nucleoside phosphorylase reactivity, respectively (Ferguson et al, 1964;Hwang et al, 1973). For FGF-23 measurements, mouse sera collected as described above were tested for FGF-23 concentration using an FGF-23 ELISA kit (Kainos Laboratories, Tokyo, Japan).…”

Section: Scanning Electron Microscopy (Sem) and Transmission Electronmentioning

confidence: 99%

Ectopic Calcification is Caused by Elevated Levels of Serum Inorganic Phosphate in Mdx Mice

Kikkawa

Ohno

Nagata

et al. 2009

Cell Struct. Funct.

View full text Add to dashboard Cite

ABSTRACT. Ectopic calcification occurs in the skeletal muscle of mdx mice, a dystrophin-deficient animal model of Duchenne muscular dystrophy. The purpose of this study was to clarify the mechanism of the calcification. The calcified deposits were identified as hydroxyapatite, a crystallized form of calcium phosphate, and the serum inorganic phosphate (Pi) level in the mdx mice was approximately 1.4 times higher than that in the normal B10 mice, suggesting that Pi plays a critical role in the ectopic calcification. When C2C12 mouse myoblasts were cultured under high-Pi conditions, myogenic differentiation was retarded while the expression of osteogenic markers such as osteocalcin and Runx2 were upregulated. This was followed by the generation of calcium deposition. Moreover, ectopic calcification reduced to an undetectable level in most of the mdx mice fed a Pireduced diet. We therefore conclude that the Pi-induced osteogenesis of muscle cells is responsible for ectopic calcification in the skeletal muscle of mdx mice.

show abstract

Section: Scanning Electron Microscopy (Sem) and Transmission Electronmentioning

confidence: 99%

Ectopic Calcification is Caused by Elevated Levels of Serum Inorganic Phosphate in Mdx Mice

Kikkawa

Ohno

Nagata

et al. 2009

Cell Struct. Funct.

View full text Add to dashboard Cite

show abstract

“…Colorimetric methods for detecting plutonium at low concentration, such as complexation with a chromotropic dye such as chlorophosphonazo-III or arsenazo-III lack specificity for oxidation states and are often subject to interferences from other metal ions and electrolyte ions [3,4].…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of Pu(III, IV, V, and VI) using a 1.0-meter liquid core waveguide

Wilson¹,

Hu²,

Nitsche

2005

Radiochimica Acta

View full text Add to dashboard Cite

Detection and quantification of the aquo ions of Pu in 1 M HClO 4 was carried out using a 1-meter liquid core waveguide (LCW) coupled to a fiber optic UV-Vis spectrometer. Detection limits of 7 × 10 -7 M for Pu(VI), 1.6 × 10 -5 M for Pu(V), 5 × 10 -6 M for Pu(IV) and 8 × 10 -6 M for Pu(III) were achieved. The limits of detection represent increases of 18 to 33 times those achievable using a conventional 1-cm path length. Because of the much lower detection limits of the LCW, routine identification of the oxidation states in dilute Pu solutions can be made.

show abstract

“…Serum Ct and CEA were analyzed using an automated chemiluminescence immunoassay system (i2000; Abbott Laboratories, Chicago, IL, USA) according to the manufacturer's protocols. The serum CA was detected using the arsenazo III method (8). The tribromoarsenazo was provided by Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Clinical significance of RET mutation screening in a pedigree of multiple endocrine neoplasia type 2A

Ying

Feng

2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract. The clinical characteristics and RET proto-oncogene (RET-PO) mutation status of a patient with multiple endocrine neoplasia type 2A pedigree (MEN2A) was analyzed with the aim of preliminarily exploring the molecular mechanisms and clinical significance of the disease. Clinical characteristics of a single MEN2A patient were analyzed. Genomic DNA was extracted from the peripheral blood of the proband and 10 family members. The 21 exons of RET-PO were PCR amplified and the amplified products were sequenced. Of the family members, 5 exhibited a C634Y (TGC→TAC) missense mutation in exon 11 of RET-PO, among which 2 family members were screened as mutation carriers, while the others did not exhibit clinical symptoms of the mutation. The screening and analysis of RET-PO mutations for the MEN2A proband and the family members suggests potential clinical phenotypes and enables assessment of the risk of disease development, thus providing useful information for determining the surgical timing of preventive thyroid gland removal.

show abstract

Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo III.

Cited by 62 publications

References 30 publications

Ectopic Calcification is Caused by Elevated Levels of Serum Inorganic Phosphate in Mdx Mice

Ectopic Calcification is Caused by Elevated Levels of Serum Inorganic Phosphate in Mdx Mice

Detection and quantification of Pu(III, IV, V, and VI) using a 1.0-meter liquid core waveguide

Clinical significance of RET mutation screening in a pedigree of multiple endocrine neoplasia type 2A

Contact Info

Product

Resources

About