Simultaneous separation of acid and basic bioactive peptides by electrodialysis with ultrafiltration membrane

Advancements in the Fractionation of Milk Biopeptides by Means of Membrane Processes

“…In this sense, two different configurations can be distinguished: electrically-enhanced filtration, which can be used with conventional pressure driven membrane filtration, and forced-flow membrane electrophoresis, which is conducted in an electrophoretic cell. Intensive researches on these membrane processes have been carried out including electromembrane filtration (EMF) [94][95], electrodialysis with UF membranes (EDUF) [96][97][98][99] and forced-flow electrophoresis (FFE) [100] for the separation of charged bioactive molecules.…”

“…The feasibility of peptide fractionation by EDUF was demonstrated notably with -Lg tryptic hydrolysate solutions and was suggested to improve the separation between basic and neutral peptides [97]. Actually, EDUF process also allowed a selective and a simultaneous separation of anionic and cationic peptides presents in an uncharacterized concentrated polypeptide mixture of snow crab byproducts hydrolysate [101].…”

Perspectives on Chemicals from Renewable Resources

“…ED shows potential of being an economically technique for the separation of charged species and has already been demonstrated at large scale for a number of applications [46]. ED separates ions in aqueous solution under the influence of a (electric) potential gradient therefore theoretically all amino acids could be separated on the basis of their isoelectric point (IP) and a number of studies have examined the isolation of amino acid containing materials using ED [47][48][49]. In practice, due to similarity in IP, only three different groups of species are isolated (acidic, basic and neutral amino acids).…”

Membranes in Biotechnology

“…The simultaneous separation of acid and base bioactive peptides by employing electrodialysis with UF was investigated by Poulin et al [47]. Peptides from a b-lactoglobulin (b-lg) hydrolysate obtained from whey were separated by using a UF membrane stacked in an electrodialysis (ED) cell.…”