Simultaneous Separation and Quantification of Iron and Transition Species Using LC-ICP-MS

Hu, Qinhong

doi:10.4236/ajac.2011.26077

Cited by 21 publications

(9 citation statements)

References 16 publications

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“…60%, Catrouillet et al, 2014) binding sites in the sample matrix will shift toward phenolic groups. Because the Fe at these sites is more tightly bound, it is perhaps not quantifiable using ferrozine (Guo et al, 2007;Hu 2011). The unpredictable endpoint of the o-PFe reaction in systems rich in humic acids (Skogerboe and Wilson, 1981;Fujisawa et al, 2011) and the apparent inability of ferrozine to compete with plant polyphenols at circumneutral pH (Guo et al, 2007) suggest that humic substances in DOM pose challenges for either optical method.…”

Section: Discussion Dissolved Organic Matter Interference On Spectropmentioning

confidence: 99%

Ionic Liquid Extraction Unveils Previously Occluded Humic‐Bound Iron in Peat Soil Pore Water

Veverica

Kane

Marcarelli

et al. 2016

Soil Science Soc of Amer J

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Globally, peatland ecosystems store tremendous amounts of C relative to their extent on the landscape, largely owing to saturated soils which limit decomposition. While there is still considerable uncertainty regarding CO 2 production potential below the water table in peatland ecosystems, extracellular Fe reduction has been suggested as a dominant pathway for anaerobic metabolism. However, colorimetric methods commonly used to quantitate Fe and partition between redox species are known to be unreliable in the presence of complex humic substances, which are common in peatland pore water. We evaluated both the standard o-phenanthroline (o-P) Method and an ionic liquid extraction (ILE) Method followed by quantitation with inductively coupled plasma optical emission spectrometry (ICP-OES) to compare total Fe recovery and Fe 2+ /Fe 3+ ratios in four distinct peatland ecosystems, ranging from rich fen to bog. While total Fe concentrations measured with ILE and o-P were correlated, the ILE method proved to be superior in both total Fe quantitation and in separately quantifying ferric (Fe 3+ ) and ferrous (Fe 2+ ) iron. In peat pore water, the o-P Method underestimated Fe 3+ by as much as 100%. A multivariate approach utilizing fluorescenceand ultraviolet (UV)-visable (Vis) spectroscopy identified indices of dissolved organic matter (DOM) humification and redox status that correlated with poor performance of the o-P Method in peat pore water. Where these interferences are present, we suggest that site-specific empirical correction factors for quantitation of total Fe by o-P can be created from ILE of Fe, but recommend ILE for accurate appraisals of iron speciation and redox processes.Abbreviations: DI, deionized; DOC, dissolved organic carbon; DOM, dissolved organic matter; EEMs, excitation-emissions matrices; FI, fluorescence index; H p , index of humification; ICP-OES, inductively coupled plasma optical emission spectrometry; ILE, ionic liquid extraction; o-P, o-phenanthroline; PARAFAC, parallel factor analysis; PCA, principal component analysis; RI, index of redox dissolved organic matter status; TEA, terminal electron acceptor; UV, ultraviolet; Vis, visible. P eatlands represent critical terrestrial C stores that are preserved by a combination of hypoxic and anoxic soil environments. These conditions present an energetic challenge for microbial consortia in anaerobic environments which utilize alternative (to oxygen) terminal electron acceptors (TEAs) during heterotrophic metabolism. In anaerobic conditions, microbes preferentially reduce several alternative TEAs for respiration, with thermodynamic yield declining in the order: NO 3 -, Mn 4+ , Fe 3+ , SO 4 2-, and ultimately CO 2 (see review by Megonigal et al., 2004). Although there is wide agreement that alternative TEA reduction is important for anaerobic decomposition, variation in the magnitudes of anaerobic CO 2 production across studies suggests that there is high variability in facultative and obligate anaerobic processes that occur below the water table (M...

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Section: Discussion Dissolved Organic Matter Interference On Spectropmentioning

confidence: 99%

Ionic Liquid Extraction Unveils Previously Occluded Humic‐Bound Iron in Peat Soil Pore Water

Veverica

Kane

Marcarelli

et al. 2016

Soil Science Soc of Amer J

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“…HPLC‐ICP mass spectrometry in low organic solvent systems can also be used for ratio determination of bound and free iron in clinical samples. The addition of pyridine‐2,6‐dicarboxylic acid into the mobile phase and the resulting anionic complexes were separated by ion exchange chromatography (IonPac Cs‐5A column) followed by ICP‐MS detection (Hu, ). An older alternative based on reductive displacement of iron by gallium was performed using ascorbate as the reducing agent to increase the sensitivity.…”

Section: The Contribution Of Icp‐ms and Atomic Spectrometry Techniquementioning

confidence: 99%

Characterization of microbial siderophores by mass spectrometry

Pluháček

Lemr

Ghosh

et al. 2015

Mass Spectrometry Reviews

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Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP-MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron-containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP-MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context.

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“…In cases where interferences occur, one can either measure a different isotope of the same element, typically 57 Fe, or switch to a dynamic reaction cell with a gas like ammonia (Hu, 2011), hydrogen, or helium (Tanner & Baranov, 1999). In ICP-MS, the detector is a mass spectrometer; after the sample has been atomized, it is passed into a vacuum where particles travel at a speed determined by their mass-to-charge ratio before striking the analyzer.…”

Section: Inductively Coupled Plasma-mass Spectrometrymentioning

confidence: 99%