Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Aksoy, Aksem

doi:10.21521/mw.6240

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…The validated method allows fast screening of nitrofuran residues from a single sample compared with other methodologies with LODs below 1 ppb. The same method was evaluated and applied to the screening of honey samples of different geographic regions and was found suitable as a screening tool for detecting nitrofuran metabolites (8,23).…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous Screening of Nitrofuran Metabolites in Honey Using Biochip Array Technology: Validation Study According to the Decision 2002/657/EC of the European Union

et al. 2022

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Background: Although no authorization is available for antibiotics to treat bee diseases, some veterinary compounds are used by beekeepers, and each country sets its own thresholds. Inappropriate and excessive use of these drugs can cause allergic reactions and antibiotic resistance in humans who consume the remaining antibiotic residues in honey and its products. It is, therefore, relevant to monitor the presence of antibiotic residues in this matrix. Objectives: A rapid method for the simultaneous screening of nitrofuran metabolite residues in honey was validated according to Commission Decision 2002/657/EC (C.D 657) and the European guideline for the validation of screening methods for veterinary medicines. Methods: This multi-analytical screening method enables the simultaneous determination of four nitrofuran metabolites [3-amino-2-oxazolidone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 1-Aminohydantoin HCl (ADH), and semicarbazide (SEC)] from a single honey sample. Thirty-five honey samples were collected randomly as real samples for screening from Tehran, IR Iran, Germany, and the Netherlands in 2018. Results: For all four antibiotic residues, the positivity threshold T was higher than the cut-off value Fm, and no false-positive results were obtained for three antibiotics (AOZ, AMOZ, and SEC). Detection capabilities (CCβ) of all compounds were under the minimum required performance limit (MRPL) authorized by the European Commission (currently 1 μg/kg). The screening results of 15 domestic and 20 imported honey samples showed that the levels of AOZ in 6.66% and 10% of the samples, the level of AMOZ in 13.33% and 0% of the samples, and the level of SEC in 33.33% and 40% of the samples were less than the cut-off ([in relative light units (RLUs)], respectively. Conclusions: This study found that this technique is valid for detecting and quantifying three antibiotic residues in honey samples at the measured validation levels. This method was simple, rapid, and capable of simultaneously screening three nitrofuran metabolites from a single honey sample.

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Section: Discussionmentioning

confidence: 99%

“…The evidence investigator analyzer is a semi-automated benchtop device. The system has clinical, forensic, and veterinary applications (3,7,8). To our knowledge, there are limited publications on detecting four nitrofuran metabolites in honey with this system (9).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Screening of Nitrofuran Metabolites in Honey Using Biochip Array Technology: Validation Study According to the Decision 2002/657/EC of the European Union

et al. 2022

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“…The results showed that residues of erythromycin, streptomycin, neomycin, tylosin B, amikacin and lincomycin were found in honey samples at different concentrations. Among them, erythromycin can be detected in 91% of local honey samples [48].…”

Section: Molecular Biological Techniquementioning

confidence: 99%

Hazards and Detection Techniques of Veterinary Drug Residues in Animal-Origin Food

Chen

2021

E3S Web Conf.

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Veterinary drugs originally used to diagnose, prevent, control and treat animal diseases. However, now some of them can also be mixed into livestock feed as growth promoters. Although most countries have specified animal-derived veterinary drug residue standards, such as the maximum residue limit, withdrawal period and prohibition of some veterinary drugs as growth promoters, there are still some animal-derived products with illegal amount of drug residues. Consequently, long-term intake of products with excessive residues will result in irreversible impacts on human health, livestock industries and natural environment. To minimize these risks, the techniques of drug residues detection plays an important role in guarding food safety of animal products. Moreover, Veterinary drug residue detection technology, according to the author's opinion, can be divided into instrumental method, immunoassay method, biomolecular technology and bio-sensor technology. The purpose of this article is to collect and summarize the existing journal literature current about status, hazards and detection techniques of animal-origin veterinary drug residues so that ordinary readers can briefly understand the research status of the residue detection techniques, farmers can understand the importance of regulating and suitable handling of veterinary drugs, and relevant researchers may know about the emphasized difficulties that need to be overcome in drug residue detection techniques.

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“…AKSEM AKSOY performed the experiment regarding screen and evaluated the presence of possible 12 different antibiotic residue simultaneously in honey based on biochip multi-array technology. Regarding to the detection analyses, the results showed that streptomycin, erythromycin, tylosin B, neomycin, lincomycin, and amikacin residues were observed in honey samples in numerous concentrations (Aksoy, 2019). Another study has been conducted by Mi-Sun Ha and for the detection of enrofloxacin by using biochips and microparticles.…”

Section: Other Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

120

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Cited by 8 publications

References 21 publications

Simultaneous Screening of Nitrofuran Metabolites in Honey Using Biochip Array Technology: Validation Study According to the Decision 2002/657/EC of the European Union

Simultaneous Screening of Nitrofuran Metabolites in Honey Using Biochip Array Technology: Validation Study According to the Decision 2002/657/EC of the European Union

Hazards and Detection Techniques of Veterinary Drug Residues in Animal-Origin Food

Current advances in immunoassays for the detection of antibiotics residues: a review

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