Simultaneous Screening and Chemical Characterization of Bioactive Compounds Using LC-MS-Based Technologies (Affinity Chromatography)

Effect-Directed Analysis of Complex Environmental Contamination

“…Since often the first step in the sequence of events that may eventually lead to an adverse affects of chemicals on biota is binding to a receptor, affinity chromatography is a promising tool in EDA. Affinity chromatography is discussed in more detail in Chapter 6 [207]. Hyphenated technologies using receptor affinity chromatography followed by LC-MS/MS for the isolation and subsequent identification of estrogenic compounds have been suggested [205,206].…”

High-Resolution Screening Combined with HPLC-HRMS-SPE-NMR for Identification of Potential Health-Promoting Constituents in Sea Aster and Searocket—New Nordic Food Ingredients

“…However, a new analytical technology platform holds promise for improved profiling of individual bioactive compounds in complex mixtures. The first component of the platform is high-resolution screening or high-resolution bioassay, − a screening technology that combines microplate-based biochemical detection with the resolution power of analytical-scale high-performance liquid chromatography. The second component is a state-of-the-art hyphenated system consisting of high-performance liquid chromatography, solid-phase extraction, and nuclear magnetic resonance, that is, HPLC-SPE-NMR, , which has proven to be successful for fast and efficient analysis of the chemical composition of pharmaceuticals and plant extracts, − including the combined use with circular dichroism to establish absolute configuration of identified metabolites .…”

“…Highresolution mass spectrometry together with detailed analysis of one-and two-dimensional proton detected NMR experiments enabled unambiguous assignment of the targeted analytes. This revealed a series of caffeoyl esters (1, 2, 5), flavonoid glycosides (3, 4, 6, 11−15), flavonoids (7−9), sinapate esters (10,16,17), and sinapinic acid (18) associated with radical scavenging and/ or α-glucosidase inhibition. In vitro assays implemented in this study showed that sea aster holds potential as a future functional food ingredient for lowering postprandial blood glucose level for diabetics, but further investigations are needed to prove the effect in vivo.…”