Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis

Abstract

Objective: The antiplatelet aggregation compounds in Rhizoma Corydalis were screened to validate its traditional medicinal use. Material and methods: Total alkaloid extract (TAE) of Rhizoma Corydalis was obtained by refluxing 100 g Rhizoma Corydalis powder with 600 mL 70% ethanol, and purified by acidification (20% HCl) and alkalization (5 M NaOH) process. Potential antiplatelet aggregation compounds in TAE were screened by a method involving platelet bio-specific extraction and HPLC-DAD/LC-MS analysis. Further in vitro antiplatelet aggregation activity confirmation of TAE and seven main alkaloids were achieved by turbidimetry method within 3 h after blood collection from rabbit carotid artery, and all the test drugs were at the concentration range of 25-350 lg/mL. Finally, HPLC-DAD was employed for the quantitative determination of seven main components in TAE. Results: Five alkaloids, identified as glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline, can be specifically extracted with platelets. The results indicated that all these five alkaloids can inhibit thrombin-induced platelet aggregation in a low dose (IC 50 of glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline were 49.057, 34.914, 33.547, 84.261 and 54.164 lg/mL, respectively) as compared to TAE (IC 50 ¼ 175.426 lg/mL) and aspirin (IC 50 ¼ 300.340 lg/mL), while the unbound compounds (palmatine and tetrahydropalmatine) had a very weak antiplatelet effect (IC 50 > 200 lg/mL). Discussion and conclusion: This study is the first reported work for antiplatelet components screening in Rhizoma Corydalis. Seven compounds were detected and identified by HPLC-DAD/LC-MS, of which five platelet-targeted compounds were discovered. ARTICLE HISTORY

Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy

“…We further identified myogenic alkaloids including stylopine, corydaline, canadine and coptisine, and canadine showed the strongest myogenic activity among the tested compounds ( Figure 2 ). Canadine has been found in herbal plants [ 32 , 33 ] showing relaxant [ 34 ], anti-oxidative [ 35 ], anti-platelet aggregation [ 36 ], anti-Alzheimer’s disease [ 37 ], anti-fibrotic and wound-healing activities [ 38 ]. In this study, we first observed the myogenesis-stimulating activity of canadine.…”

<p>Traditional Chinese Medicine Jiuwei Zhenxin Granules in Treating Depression: An Overview</p>

“…A total of more than 20 alkaloids have been identified from the tubers of CYHS, including corydaline, dl-tetrahydropalmatine, protopine, L-tetraline, L-tetrahydrocoptisine (L-stylopin), dl-tetrahydroberberine, L-tetrahydrocolumbamine, corybulbine, β-herbadol, β-homoche-lidonine, coptisine, dehydrocorydaline, and corydalmine. [82][83][84][85] CYHS is currently used clinically, mainly for the treatment of drug addiction, 86 as well as for its antiaging, anxiolytic, 87 and antiplatelet aggregation 88 properties and for the treatment of depression. 89 Qi stagnation and blood stasis are primary causes of depression in the theory of TCM.…”

“…CYHS is currently used clinically, mainly for the treatment of drug addiction, 86 as well as for its antiaging, anxiolytic, 87 and antiplatelet aggregation 88 properties and for the treatment of depression. 89 Qi stagnation and blood stasis are primary causes of depression in the theory of TCM.…”

Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity

“…These have a wide range of pharmacological activities such as: Antioxidant, antibacterial, antiviral, anticancer, analgesic, and promotion of blood circulation. For analysis of alkaloids in extracts obtained from Corydalis species, C18 column and mobile phases consisted of acetonitrile, water and formic acid [19,20,21,22], ammonium acetate [23], formic acid and ammonium acetate [24], acetate buffer at pH 5.0 [20], ammonium hydroxide [25], acetonitrile, water, acetic acid, and diethylamine [26], or acetic acid with triethylamine [27] rarely methanol, water with the addition of formic acid [28] or formic acid and ammonium acetate [29] were applied. The inhibitory effects on human tumor cell lines: A549, SK-OV-3, SK-MEL-2, and HCT-15 of tubers of Corydalis ternata were found [30].…”