Simultaneous Saccharification and Fermentation and Factors Influencing Ethanol Production in SSF Process

Kanagasabai, Manikandan; Karuppaiya, M.; Viruthagiri, T.

doi:10.5772/intechopen.86480

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…Response surface methodology can be employed to carry out optimization statistically by carefully designing the experiments. The RSM yields best combination of pH, temperature, initial starch concentration and enzyme concentration that give maximum ethanol concentration in the fermenter [20]. The interactive effects of process variables can be analyzed very effectively in the single step simultaneous saccharification and fermentation process.…”

Section: Saccharification and Fermentation Of Starchmentioning

confidence: 99%

Ethanol Production from Bioresources and Its Kinetic Modeling: Optimization Methods

Kanagasabai¹,

Babu²,

Balakrishnan³

et al. 2023

Ethanol and Glycerol Chemistry - Production, Modelling, Applications, and Technological Aspects

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Ethanol is viable alternative fuel and it’s substitute to fossil fuel has gained importance with rise in fuel prices. The chapter elaborates about methods of production from different types of bio resources like molasses, starch and cellulose commercially. The chapter also details about different methods of pretreatment for cellulisic and starchy raw materials. This also includes hydrolysis using acid and enzymes. The modes of ethanol fermentation using bioreactors like batch fed batch and continuous operation will be discussed. The growth kinetics models like monad logistic model will be elaborated. The product formation growth associated models like Leudiking piret model and parameter estimation methods will be described. Optimization of process variables using response surface methodology and media optimization using PB design will be elaborated. The application of ANN in modeling will be described.

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Section: Saccharification and Fermentation Of Starchmentioning

confidence: 99%

Ethanol Production from Bioresources and Its Kinetic Modeling: Optimization Methods

Kanagasabai¹,

Babu²,

Balakrishnan³

et al. 2023

Ethanol and Glycerol Chemistry - Production, Modelling, Applications, and Technological Aspects

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“…Interestingly, microbial consortia DM-1 decomposed 14.0% of lignin with no significant cellulose degradation for initial 20 days (Lin et al, 2020). Besides, the SSF (simultaneous saccharification and fermentation) method revealed 20% increment in ethanol production with DM-1 treated microbial consortia as compared to control (Kanagasabai et al, 2019). Similarly, pretreatment by a novel microbial consortium was investigated for its influence on methane production (Raut et al, 2021).…”

Section: Microbial Consortiamentioning

confidence: 99%

Endophytes in Lignin Valorization: A Novel Approach

Mattoo

Nonzom

2022

Front. Bioeng. Biotechnol.

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Lignin, one of the essential components of lignocellulosic biomass, comprises an abundant renewable aromatic resource on the planet earth. Although 15%––40% of lignocellulose pertains to lignin, its annual valorization rate is less than 2% which raises the concern to harness and/or develop effective technologies for its valorization. The basic hindrance lies in the structural heterogeneity, complexity, and stability of lignin that collectively makes it difficult to depolymerize and yield common products. Recently, microbial delignification, an eco-friendly and cheaper technique, has attracted the attention due to the diverse metabolisms of microbes that can channelize multiple lignin-based products into specific target compounds. Also, endophytes, a fascinating group of microbes residing asymptomatically within the plant tissues, exhibit marvellous lignin deconstruction potential. Apart from novel sources for potent and stable ligninases, endophytes share immense ability of depolymerizing lignin into desired valuable products. Despite their efficacy, ligninolytic studies on endophytes are meagre with incomplete understanding of the pathways involved at the molecular level. In the recent years, improvement of thermochemical methods has received much attention, however, we lagged in exploring the novel microbial groups for their delignification efficiency and optimization of this ability. This review summarizes the currently available knowledge about endophytic delignification potential with special emphasis on underlying mechanism of biological funnelling for the production of valuable products. It also highlights the recent advancements in developing the most intriguing methods to depolymerize lignin. Comparative account of thermochemical and biological techniques is accentuated with special emphasis on biological/microbial degradation. Exploring potent biological agents for delignification and focussing on the basic challenges in enhancing lignin valorization and overcoming them could make this renewable resource a promising tool to accomplish Sustainable Development Goals (SDG’s) which are supposed to be achieved by 2030.

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“…The unsuitable temperature for the microbial growth causes an inhibitory effect on the production of bioethanol (Selim et al, 2018). The increment of temperature improves the rate of biological reactions up to a certain temperature but further increment in temperature may cause in lesser product formation (Kanagasabai et al, 2019). The duration of saccharification was manipulated from 1 day to 5 days using the optimal fermentation temperature, with A. niger B2484 producing 0.04 ± 0.01 g/L ethanol after 3 days, while T. asperellum B1581 produced most ethanol (0.05 ± 0.01 g/L) after 2 days of saccharification (Figure 1b).…”

Section: Determination Of Parameters Via Ofat Analysismentioning

confidence: 99%

Improvement of Bioethanol Production in Consolidated Bioprocessing (CBP) via Consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581

Ghazali¹,

Mustafa²,

Zainudin³

et al. 2021

JST

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Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor, using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However, the main disadvantage of this process is the complexity to optimise all factors considering both enzymes and microbial activity at the same time. Hence, this study aimed to optimise suitable culture conditions for both organisms to work efficiently. Six single factors that are considered crucial for bioethanol production were tested in one-factor-at-a-time (OFAT) analysis and analysed using Response Surface Methodology (RSM) software for Aspergillus niger B2484 and Trichoderma asperellum B1581 strains. The formulation of a new consortia setting was developed based on the average of two settings generated from RSM testing several combinations of consortia concentrations (5:1, 2:4, 3:3, 4:2, and 1:5). The combination of 5:1 Aspergillus niger B2484 and Trichoderma asperellum B1581 produced the most ethanol with 1.03 g/L, more than A. niger B2484, alone with 0.34 g/L of ethanol, indicating the potential of the combination of A. niger B2484 and T. asperellum B1581 co-culture for bioethanol production in CBP.

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Simultaneous Saccharification and Fermentation and Factors Influencing Ethanol Production in SSF Process

Cited by 9 publications

References 33 publications

Ethanol Production from Bioresources and Its Kinetic Modeling: Optimization Methods

Ethanol Production from Bioresources and Its Kinetic Modeling: Optimization Methods

Endophytes in Lignin Valorization: A Novel Approach

Improvement of Bioethanol Production in Consolidated Bioprocessing (CBP) via Consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581

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